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- PDB-2rqw: Solution structure of Bem1p SH3CI domain complexed with Ste20p-PR... -
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Basic information
Entry | Database: PDB / ID: 2rqw | ||||||
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Title | Solution structure of Bem1p SH3CI domain complexed with Ste20p-PRR peptide | ||||||
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![]() | SIGNALING PROTEIN / Bem1p / Ste20p / SH3CI / PRR / Cytoplasm / Cytoskeleton / SH3 domain / Cell cycle / Pheromone response / Serine/threonine-protein kinase | ||||||
Function / homology | ![]() sterol import / histone H2BS14 kinase activity / CD28 dependent Vav1 pathway / osmosensory signaling pathway via Sho1 osmosensor / RHO GTPases activate PAKs / signal transduction involved in filamentous growth / bipolar cellular bud site selection / RHO GTPases Activate NADPH Oxidases / RHOD GTPase cycle / RHOV GTPase cycle ...sterol import / histone H2BS14 kinase activity / CD28 dependent Vav1 pathway / osmosensory signaling pathway via Sho1 osmosensor / RHO GTPases activate PAKs / signal transduction involved in filamentous growth / bipolar cellular bud site selection / RHO GTPases Activate NADPH Oxidases / RHOD GTPase cycle / RHOV GTPase cycle / budding cell apical bud growth / conjugation with cellular fusion / RHOQ GTPase cycle / cell morphogenesis involved in conjugation with cellular fusion / RHOU GTPase cycle / Regulation of actin dynamics for phagocytic cup formation / PAR polarity complex / pseudohyphal growth / site of polarized growth / superoxide-generating NADPH oxidase activator activity / pheromone-dependent signal transduction involved in conjugation with cellular fusion / invasive growth in response to glucose limitation / vacuole inheritance / cellular bud site selection / incipient cellular bud site / cellular bud tip / maintenance of protein location / regulation of exit from mitosis / cellular bud neck / mating projection tip / regulation of Rho protein signal transduction / phosphatidylinositol-3-phosphate binding / superoxide anion generation / regulation of axonogenesis / MAPK6/MAPK4 signaling / regulation of MAPK cascade / stress granule assembly / protein-macromolecule adaptor activity / cell cortex / molecular adaptor activity / cytoskeleton / non-specific serine/threonine protein kinase / protein kinase activity / intracellular signal transduction / positive regulation of apoptotic process / phosphorylation / negative regulation of gene expression / protein serine kinase activity / protein serine/threonine kinase activity / mRNA binding / negative regulation of transcription by RNA polymerase II / mitochondrion / ATP binding / nucleus / plasma membrane / cytoplasm Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | SOLUTION NMR / torsion angle dynamics | ||||||
Model details | lowest energy, model 1 | ||||||
![]() | Takaku, T. / Ogura, K. / Inagaki, F. | ||||||
![]() | ![]() Title: Solution structure of a novel Cdc42-binding module of Bem1 and its interaction with Ste20 and Cdc42 Authors: Takaku, T. / Ogura, K. / Kumeta, H. / Yoshida, N. / Inagaki, F. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 801.6 KB | Display | ![]() |
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PDB format | ![]() | 679.5 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
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-Validation report
Summary document | ![]() | 353.9 KB | Display | ![]() |
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Full document | ![]() | 626.8 KB | Display | |
Data in XML | ![]() | 74.1 KB | Display | |
Data in CIF | ![]() | 95 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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NMR ensembles |
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Components
#1: Protein | Mass: 11414.939 Da / Num. of mol.: 1 / Fragment: SH3CI domain, residues 156-260 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Production host: ![]() ![]() |
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#2: Protein/peptide | Mass: 2331.562 Da / Num. of mol.: 1 / Fragment: BEM1-binding domain, residues 463-486 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Production host: ![]() ![]() |
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||
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NMR experiment |
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Sample preparation
Details | Contents: 0.5mM [U-99% 13C; U-99% 15N] protein; 90% H2O/10% D2O Solvent system: 90% H2O/10% D2O |
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Sample | Conc.: 0.5 mM / Component: entity-1 / Isotopic labeling: [U-99% 13C; U-99% 15N] |
Sample conditions | Ionic strength: 2 / pH: 6.5 / Pressure: ambient / Temperature: 298 K |
-NMR measurement
NMR spectrometer | Type: Varian INOVA / Manufacturer: Varian / Model: INOVA / Field strength: 800 MHz |
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Processing
NMR software |
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Refinement | Method: torsion angle dynamics / Software ordinal: 1 | ||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||
NMR ensemble | Conformer selection criteria: target function / Conformers calculated total number: 100 / Conformers submitted total number: 20 / Representative conformer: 1 |