+Open data
-Basic information
Entry | Database: PDB / ID: 1jt8 | ||||||
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Title | ARCHAEAL INITIATION FACTOR-1A, AIF-1A | ||||||
Components | PROBABLE TRANSLATION INITIATION FACTOR 1A | ||||||
Keywords | TRANSLATION / beta barrel / Translation factor | ||||||
Function / homology | Function and homology information | ||||||
Biological species | Methanocaldococcus jannaschii (archaea) | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
Authors | Hoffman, D.W. / Li, W. | ||||||
Citation | Journal: Protein Sci. / Year: 2001 Title: Structure and dynamics of translation initiation factor aIF-1A from the archaeon Methanococcus jannaschii determined by NMR spectroscopy Authors: Li, W. / Hoffman, D.W. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1jt8.cif.gz | 346.1 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1jt8.ent.gz | 285.5 KB | Display | PDB format |
PDBx/mmJSON format | 1jt8.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1jt8_validation.pdf.gz | 348 KB | Display | wwPDB validaton report |
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Full document | 1jt8_full_validation.pdf.gz | 444.9 KB | Display | |
Data in XML | 1jt8_validation.xml.gz | 24.4 KB | Display | |
Data in CIF | 1jt8_validation.cif.gz | 38 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/jt/1jt8 ftp://data.pdbj.org/pub/pdb/validation_reports/jt/1jt8 | HTTPS FTP |
-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 12205.257 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Methanocaldococcus jannaschii (archaea) Production host: Escherichia coli (E. coli) / References: UniProt: Q57887 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||
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NMR experiment |
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NMR details | Text: This structure was determined using standard NMR techniques. |
-Sample preparation
Details | Contents: 10 mM sodium phosphate, 300 mM sodium chloride, 5 mM sodium azide, pH 6 Solvent system: 90% H2O/10% D2O |
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Sample conditions | Ionic strength: 300 mM NaCl / pH: 6.0 / Pressure: ambient / Temperature: 303 K |
Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M |
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Radiation wavelength | Relative weight: 1 |
NMR spectrometer | Type: Varian INOVA / Manufacturer: Varian / Model: INOVA / Field strength: 500 MHz |
-Processing
NMR software | Name: X-PLOR / Version: 3.1 / Developer: Brunger / Classification: refinement |
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Refinement | Method: simulated annealing / Software ordinal: 1 Details: 943 NOE-derived distances restraints, 88 dihedral angle restraints, 39 hydrogen bond restraints |
NMR representative | Selection criteria: lowest energy |
NMR ensemble | Conformer selection criteria: structures with acceptable covalent geometry Conformers calculated total number: 240 / Conformers submitted total number: 10 |