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Yorodumi- PDB-2rql: Solution structure of the E. coli ribosome hibernation promoting ... -
+Open data
-Basic information
Entry | Database: PDB / ID: 2rql | ||||||
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Title | Solution structure of the E. coli ribosome hibernation promoting factor HPF | ||||||
Components | Probable sigma-54 modulation protein | ||||||
Keywords | TRANSLATION / ribosome hibernation promoting factor / HPF / ribosome | ||||||
Function / homology | Function and homology information dormancy process / negative regulation of translational elongation / primary metabolic process / ribosomal small subunit binding / cytosolic small ribosomal subunit / ribosome binding / regulation of translation / negative regulation of translation / cytosol Similarity search - Function | ||||||
Biological species | Escherichia coli (E. coli) | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
Model details | lowest energy, model 1 | ||||||
Authors | Sato, A. / Mishima, M. | ||||||
Citation | Journal: Biochem.Biophys.Res.Commun. / Year: 2009 Title: Solution structure of the E. coli ribosome hibernation promoting factor HPF: Implications for the relationship between structure and function. Authors: Sato, A. / Watanabe, T. / Maki, Y. / Ueta, M. / Yoshida, H. / Ito, Y. / Wada, A. / Mishima, M. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2rql.cif.gz | 584.3 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2rql.ent.gz | 509.9 KB | Display | PDB format |
PDBx/mmJSON format | 2rql.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/rq/2rql ftp://data.pdbj.org/pub/pdb/validation_reports/rq/2rql | HTTPS FTP |
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-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 10767.245 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli (E. coli) / Gene: hpf, ECs4082 / Production host: Escherichia coli (E. coli) / Strain (production host): M15 / References: UniProt: Q2KHI5, UniProt: P0AFX0*PLUS |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Details |
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Sample |
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Sample conditions | Ionic strength: 0.02 / pH: 6.9 / Pressure: ambient atm / Temperature: 303 K |
-NMR measurement
NMR spectrometer | Type: Bruker Avance / Manufacturer: Bruker / Model: AVANCE / Field strength: 600 MHz |
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-Processing
NMR software |
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Refinement | Method: simulated annealing / Software ordinal: 1 | ||||||||||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 100 / Conformers submitted total number: 20 / Representative conformer: 1 |