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Yorodumi- PDB-2rn4: Solution structure of the alkaline proteinase inhibitor APRin fro... -
+Open data
-Basic information
Entry | Database: PDB / ID: 2rn4 | ||||||
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Title | Solution structure of the alkaline proteinase inhibitor APRin from Pseudomonas aeruginosa | ||||||
Components | Proteinase inhibitor | ||||||
Keywords | HYDROLASE INHIBITOR / protein / Metalloenzyme inhibitor / Metalloprotease inhibitor / Periplasm | ||||||
Function / homology | Function and homology information | ||||||
Biological species | Pseudomonas aeruginosa (bacteria) | ||||||
Method | SOLUTION NMR / DGSA-distance geometry simulated annealing, simulated annealing | ||||||
Authors | Arumugam, S. / Gray, R.D. / Lane, A.N. | ||||||
Citation | Journal: J.Biomol.Nmr / Year: 2008 Title: NMR structure note: alkaline proteinase inhibitor APRin from Pseudomonas aeruginosa. Authors: Arumugam, S. / Gray, R.D. / Lane, A.N. #1: Journal: J.Biomol.Nmr / Year: 2005 Title: 1H, 15N and 13C assignments of the alkaline proteinase inhibitor APRin from Pseudomonas aeruginosa. Authors: Arumugam, S. / Gray, R.D. / Lane, A.N. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2rn4.cif.gz | 617.6 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2rn4.ent.gz | 534 KB | Display | PDB format |
PDBx/mmJSON format | 2rn4.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 2rn4_validation.pdf.gz | 341.2 KB | Display | wwPDB validaton report |
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Full document | 2rn4_full_validation.pdf.gz | 495.9 KB | Display | |
Data in XML | 2rn4_validation.xml.gz | 38.5 KB | Display | |
Data in CIF | 2rn4_validation.cif.gz | 60.8 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/rn/2rn4 ftp://data.pdbj.org/pub/pdb/validation_reports/rn/2rn4 | HTTPS FTP |
-Related structure data
Similar structure data | |
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Other databases |
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 11396.819 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Pseudomonas aeruginosa (bacteria) / Gene: inh, aprI / Production host: Escherichia coli (E. coli) / Strain (production host): BL21 / References: UniProt: Q03026 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Details |
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Sample |
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Sample conditions | Ionic strength: 0.24 / pH: 6.5 / Pressure: ambient / Temperature: 298 K |
-NMR measurement
NMR spectrometer | Type: Varian INOVA / Manufacturer: Varian / Model: INOVA / Field strength: 800 MHz |
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-Processing
NMR software |
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Refinement | Method: DGSA-distance geometry simulated annealing, simulated annealing Software ordinal: 1 | ||||||||||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 100 / Conformers submitted total number: 20 |