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Yorodumi- PDB-2ran: RAT ANNEXIN V CRYSTAL STRUCTURE: CA2+-INDUCED CONFORMATIONAL CHANGES -
+Open data
-Basic information
Entry | Database: PDB / ID: 2ran | ||||||
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Title | RAT ANNEXIN V CRYSTAL STRUCTURE: CA2+-INDUCED CONFORMATIONAL CHANGES | ||||||
Components | ANNEXIN V | ||||||
Keywords | CALCIUM/PHOSPHOLIPID-BINDING PROTEIN / CALCIUM-PHOSPHOLIPID-BINDING PROTEIN complex | ||||||
Function / homology | Function and homology information negative regulation of prolactin secretion / cellular response to gonadotropin-releasing hormone / regulation of flagellated sperm motility / Platelet degranulation / endothelial microparticle / cellular response to lead ion / P-type calcium transporter activity / response to thyroid hormone / calcium-dependent phospholipid binding / negative regulation of blood coagulation ...negative regulation of prolactin secretion / cellular response to gonadotropin-releasing hormone / regulation of flagellated sperm motility / Platelet degranulation / endothelial microparticle / cellular response to lead ion / P-type calcium transporter activity / response to thyroid hormone / calcium-dependent phospholipid binding / negative regulation of blood coagulation / phosphatidylserine binding / peptide hormone binding / intercalated disc / axon terminus / : / cell projection / receptor tyrosine kinase binding / sarcolemma / synaptic vesicle membrane / Z disc / response to calcium ion / blood coagulation / synaptic vesicle / perikaryon / positive regulation of apoptotic process / external side of plasma membrane / neuronal cell body / calcium ion binding / dendrite / extracellular space / identical protein binding / cytoplasm Similarity search - Function | ||||||
Biological species | Rattus norvegicus (Norway rat) | ||||||
Method | X-RAY DIFFRACTION / Resolution: 1.89 Å | ||||||
Authors | Concha, N.O. / Head, J.F. / Kaetzel, M.A. / Dedman, J.R. / Seaton, B.A. | ||||||
Citation | Journal: Science / Year: 1993 Title: Rat annexin V crystal structure: Ca(2+)-induced conformational changes. Authors: Concha, N.O. / Head, J.F. / Kaetzel, M.A. / Dedman, J.R. / Seaton, B.A. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2ran.cif.gz | 90.8 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2ran.ent.gz | 68.6 KB | Display | PDB format |
PDBx/mmJSON format | 2ran.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 2ran_validation.pdf.gz | 382.7 KB | Display | wwPDB validaton report |
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Full document | 2ran_full_validation.pdf.gz | 390.2 KB | Display | |
Data in XML | 2ran_validation.xml.gz | 8.6 KB | Display | |
Data in CIF | 2ran_validation.cif.gz | 12.7 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ra/2ran ftp://data.pdbj.org/pub/pdb/validation_reports/ra/2ran | HTTPS FTP |
-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 35427.090 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Rattus norvegicus (Norway rat) / References: UniProt: P14668 | ||||
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#2: Chemical | ChemComp-CA / #3: Chemical | #4: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
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-Sample preparation
Crystal | Density Matthews: 2.48 Å3/Da / Density % sol: 50.32 % | ||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | *PLUS Method: vapor diffusion, hanging dropDetails: or microdialysis taken from Seaton, B.A. (1990) J. Biol. Chem., 265, 4567. PH range low: 8 / PH range high: 7.5 | ||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Reflection | Num. obs: 20818 / % possible obs: 76 % |
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Reflection | *PLUS Highest resolution: 1.89 Å / Lowest resolution: 21.32 Å / Observed criterion σ(I): 2 |
-Processing
Software |
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Refinement | Resolution: 1.89→21.32 Å / σ(F): 2
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Refinement step | Cycle: LAST / Resolution: 1.89→21.32 Å
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Refine LS restraints |
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Software | *PLUS Name: X-PLOR / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS Highest resolution: 2 Å / Lowest resolution: 6 Å / Rfactor obs: 0.212 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS Type: x_angle_d / Dev ideal: 2.9 |