[English] 日本語
![](img/lk-miru.gif)
- PDB-2r5z: Structure of Scr/Exd complex bound to a DNA sequence derived from... -
+
Open data
-
Basic information
Entry | Database: PDB / ID: 2r5z | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
Title | Structure of Scr/Exd complex bound to a DNA sequence derived from the fkh gene | |||||||||
![]() |
| |||||||||
![]() | TRANSCRIPTION/DNA / Homeodomain / homeotic proteins / specificity / Developmental protein / DNA-binding / Homeobox / Nucleus / Transcription / Transcription regulation / Transcription-DNA COMPLEX | |||||||||
Function / homology | ![]() sex comb development / salivary gland boundary specification / oenocyte development / somatic muscle development / eye development / regulation of cell fate specification / peripheral nervous system development / anterior/posterior pattern specification / midgut development / transcription factor binding ...sex comb development / salivary gland boundary specification / oenocyte development / somatic muscle development / eye development / regulation of cell fate specification / peripheral nervous system development / anterior/posterior pattern specification / midgut development / transcription factor binding / neuron development / embryonic organ development / cis-regulatory region sequence-specific DNA binding / protein-DNA complex / transcription coregulator activity / animal organ morphogenesis / RNA polymerase II transcription regulatory region sequence-specific DNA binding / brain development / RNA polymerase II transcription regulator complex / DNA-binding transcription factor binding / transcription regulator complex / sequence-specific DNA binding / transcription coactivator activity / DNA-binding transcription factor activity, RNA polymerase II-specific / protein heterodimerization activity / RNA polymerase II cis-regulatory region sequence-specific DNA binding / DNA-binding transcription factor activity / regulation of transcription by RNA polymerase II / positive regulation of DNA-templated transcription / protein homodimerization activity / positive regulation of transcription by RNA polymerase II / DNA binding / nucleoplasm / nucleus / cytoplasm Similarity search - Function | |||||||||
Biological species | ![]() ![]() synthetic construct (others) | |||||||||
Method | ![]() ![]() ![]() | |||||||||
![]() | Aggarwal, A.K. / Passner, J.M. / Jain, R. | |||||||||
![]() | ![]() Title: Functional specificity of a Hox protein mediated by the recognition of minor groove structure Authors: Joshi, R. / Passner, J.M. / Rohs, R. / Jain, R. / Sosinsky, A. / Crickmore, M.A. / Jacob, V. / Aggarwal, A.K. / Honig, B. / Mann, R.S. | |||||||||
History |
|
-
Structure visualization
Structure viewer | Molecule: ![]() ![]() |
---|
-
Downloads & links
-
Download
PDBx/mmCIF format | ![]() | 68.3 KB | Display | ![]() |
---|---|---|---|---|
PDB format | ![]() | 46.9 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 445.1 KB | Display | ![]() |
---|---|---|---|---|
Full document | ![]() | 452.3 KB | Display | |
Data in XML | ![]() | 10.9 KB | Display | |
Data in CIF | ![]() | 14.6 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 2r5yC ![]() 1b8iS S: Starting model for refinement C: citing same article ( |
---|---|
Similar structure data |
-
Links
-
Assembly
Deposited unit | ![]()
| ||||||||
---|---|---|---|---|---|---|---|---|---|
1 |
| ||||||||
Unit cell |
|
-
Components
#1: DNA chain | Mass: 6132.991 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) / References: ![]() |
---|---|
#2: DNA chain | Mass: 6132.991 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) / References: ![]() |
#3: Protein | Mass: 10967.711 Da / Num. of mol.: 1 / Fragment: Homeobox DNA-binding domain Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
#4: Protein | Mass: 7553.561 Da / Num. of mol.: 1 / Fragment: Homeobox TALE-type DNA-binding domain Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
#5: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
---|
-
Sample preparation
Crystal | Density Matthews: 2.63 Å3/Da / Density % sol: 53.29 % |
---|---|
Crystal grow | pH: 8.7 Details: 10-14 % PEG 4K, 20 % MPD, 0.1 M Tris, pH 8.7-8.9, 0.2 M Sodium Acetate, 0.2 M Potassium Chloride |
-Data collection
Diffraction | Mean temperature: 110 K |
---|---|
Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 315 / Detector: CCD / Date: Sep 10, 2004 Details: Rosenbaum-Rock high-resolution double-crystal monochromator. |
Radiation | Monochromator: Rosenbaum-Rock high-resolution double-crystal monochromator. Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.1 Å / Relative weight: 1 |
Reflection | Resolution: 2.6→50 Å / Num. all: 10408 / Num. obs: 10189 / % possible obs: 97.9 % / Observed criterion σ(F): -3 / Redundancy: 33.6 % / Biso Wilson estimate: 41.7 Å2 / Rmerge(I) obs: 0.063 / Rsym value: 0.063 / Net I/σ(I): 36 |
Reflection shell | Resolution: 2.6→2.69 Å / Redundancy: 1.86 % / Rmerge(I) obs: 0.32 / Mean I/σ(I) obs: 9.7 / Num. unique all: 1008 / Rsym value: 0.32 / % possible all: 100 |
-
Processing
Software |
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method to determine structure: ![]() Starting model: PDB entry 1B8I Resolution: 2.6→11.98 Å / Rfactor Rfree error: 0.013 / Data cutoff high absF: 1949550.74 / Data cutoff low absF: 0 / Isotropic thermal model: RESTRAINED / Cross valid method: THROUGHOUT / σ(F): 0 / Stereochemistry target values: Engh & Huber
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | Solvent model: FLAT MODEL / Bsol: 45.4238 Å2 / ksol: 0.325294 e/Å3 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 57.3 Å2
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine analyze |
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.6→11.98 Å
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints |
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
LS refinement shell | Resolution: 2.6→2.76 Å / Rfactor Rfree error: 0.042 / Total num. of bins used: 6
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Xplor file |
|