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- PDB-2qxs: Crystal Structure of Antagonizing Mutant 536S of the Estrogen Rec... -

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Basic information

Entry
Database: PDB / ID: 2qxs
TitleCrystal Structure of Antagonizing Mutant 536S of the Estrogen Receptor Alpha Ligand Binding Domain Complexed to Raloxifene
ComponentsEstrogen receptor
KeywordsTRANSCRIPTION / Protein-Ligand Complex / DNA-binding / Lipid-binding / Metal-binding / Nucleus / Phosphorylation / Receptor / Steroid-binding / Transcription regulation / Zinc-finger
Function / homology
Function and homology information


regulation of epithelial cell apoptotic process / antral ovarian follicle growth / G protein-coupled estrogen receptor activity / regulation of branching involved in prostate gland morphogenesis / RUNX1 regulates transcription of genes involved in WNT signaling / RUNX1 regulates estrogen receptor mediated transcription / regulation of toll-like receptor signaling pathway / prostate epithelial cord arborization involved in prostate glandular acinus morphogenesis / nuclear estrogen receptor activity / epithelial cell proliferation involved in mammary gland duct elongation ...regulation of epithelial cell apoptotic process / antral ovarian follicle growth / G protein-coupled estrogen receptor activity / regulation of branching involved in prostate gland morphogenesis / RUNX1 regulates transcription of genes involved in WNT signaling / RUNX1 regulates estrogen receptor mediated transcription / regulation of toll-like receptor signaling pathway / prostate epithelial cord arborization involved in prostate glandular acinus morphogenesis / nuclear estrogen receptor activity / epithelial cell proliferation involved in mammary gland duct elongation / epithelial cell development / prostate epithelial cord elongation / negative regulation of smooth muscle cell apoptotic process / mammary gland branching involved in pregnancy / uterus development / vagina development / TFIIB-class transcription factor binding / androgen metabolic process / steroid hormone receptor signaling pathway / mammary gland alveolus development / estrogen receptor signaling pathway / cellular response to estrogen stimulus / estrogen response element binding / : / nuclear receptor-mediated steroid hormone signaling pathway / negative regulation of canonical NF-kappaB signal transduction / Nuclear signaling by ERBB4 / RNA polymerase II preinitiation complex assembly / protein localization to chromatin / 14-3-3 protein binding / TBP-class protein binding / nitric-oxide synthase regulator activity / steroid binding / TFAP2 (AP-2) family regulates transcription of growth factors and their receptors / ESR-mediated signaling / transcription corepressor binding / negative regulation of miRNA transcription / positive regulation of nitric-oxide synthase activity / cellular response to estradiol stimulus / transcription coregulator binding / stem cell differentiation / nuclear estrogen receptor binding / SUMOylation of intracellular receptors / euchromatin / negative regulation of DNA-binding transcription factor activity / beta-catenin binding / transcription coactivator binding / positive regulation of DNA-binding transcription factor activity / Nuclear Receptor transcription pathway / response to estrogen / Regulation of RUNX2 expression and activity / Constitutive Signaling by Aberrant PI3K in Cancer / nuclear receptor activity / male gonad development / positive regulation of nitric oxide biosynthetic process / sequence-specific double-stranded DNA binding / positive regulation of fibroblast proliferation / Ovarian tumor domain proteases / PIP3 activates AKT signaling / response to estradiol / phospholipase C-activating G protein-coupled receptor signaling pathway / positive regulation of cytosolic calcium ion concentration / ATPase binding / regulation of inflammatory response / fibroblast proliferation / PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling / DNA-binding transcription activator activity, RNA polymerase II-specific / Estrogen-dependent gene expression / transcription regulator complex / Extra-nuclear estrogen signaling / calmodulin binding / DNA-binding transcription factor activity, RNA polymerase II-specific / chromatin remodeling / RNA polymerase II cis-regulatory region sequence-specific DNA binding / DNA-binding transcription factor activity / negative regulation of gene expression / chromatin binding / regulation of DNA-templated transcription / chromatin / regulation of transcription by RNA polymerase II / protein kinase binding / positive regulation of DNA-templated transcription / Golgi apparatus / enzyme binding / negative regulation of transcription by RNA polymerase II / signal transduction / positive regulation of transcription by RNA polymerase II / protein-containing complex / zinc ion binding / nucleoplasm / identical protein binding / membrane / nucleus / plasma membrane / cytoplasm / cytosol
Similarity search - Function
Estrogen receptor / Oestrogen-type nuclear receptor final C-terminal domain / : / Oestrogen receptor / Oestrogen-type nuclear receptor final C-terminal / Estrogen receptor/oestrogen-related receptor / Retinoid X Receptor / Retinoid X Receptor / Nuclear hormone receptor / Nuclear hormones receptors DNA-binding region signature. ...Estrogen receptor / Oestrogen-type nuclear receptor final C-terminal domain / : / Oestrogen receptor / Oestrogen-type nuclear receptor final C-terminal / Estrogen receptor/oestrogen-related receptor / Retinoid X Receptor / Retinoid X Receptor / Nuclear hormone receptor / Nuclear hormones receptors DNA-binding region signature. / Zinc finger, nuclear hormone receptor-type / Zinc finger, C4 type (two domains) / Nuclear hormone receptors DNA-binding domain profile. / c4 zinc finger in nuclear hormone receptors / Nuclear hormone receptor, ligand-binding domain / Nuclear hormone receptor-like domain superfamily / Ligand-binding domain of nuclear hormone receptor / Nuclear receptor (NR) ligand-binding (LBD) domain profile. / Ligand binding domain of hormone receptors / Zinc finger, NHR/GATA-type / Orthogonal Bundle / Mainly Alpha
Similarity search - Domain/homology
RALOXIFENE / Estrogen receptor
Similarity search - Component
Biological speciesHomo sapiens (human)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / molecular replacement / Resolution: 1.7 Å
AuthorsBruning, J.B. / Gil, G. / Nowak, J. / Katzenellenbogen, J. / Nettles, K.W.
CitationJournal: Nat.Chem.Biol. / Year: 2010
Title: Coupling of receptor conformation and ligand orientation determine graded activity.
Authors: Bruning, J.B. / Parent, A.A. / Gil, G. / Zhao, M. / Nowak, J. / Pace, M.C. / Smith, C.L. / Afonine, P.V. / Adams, P.D. / Katzenellenbogen, J.A. / Nettles, K.W.
History
DepositionAug 12, 2007Deposition site: RCSB / Processing site: RCSB
Revision 1.0Sep 2, 2008Provider: repository / Type: Initial release
Revision 1.1Jul 13, 2011Group: Version format compliance
Revision 2.0Oct 25, 2017Group: Advisory / Atomic model ...Advisory / Atomic model / Refinement description / Source and taxonomy
Category: atom_site / pdbx_entity_src_syn ...atom_site / pdbx_entity_src_syn / pdbx_unobs_or_zero_occ_atoms / software
Item: _atom_site.occupancy / _pdbx_entity_src_syn.details ..._atom_site.occupancy / _pdbx_entity_src_syn.details / _pdbx_entity_src_syn.ncbi_taxonomy_id / _pdbx_entity_src_syn.organism_common_name / _pdbx_entity_src_syn.organism_scientific / _software.classification / _software.contact_author / _software.contact_author_email / _software.date / _software.language / _software.location / _software.name / _software.type / _software.version
Revision 2.1Oct 20, 2021Group: Advisory / Database references / Derived calculations
Category: database_2 / pdbx_unobs_or_zero_occ_atoms ...database_2 / pdbx_unobs_or_zero_occ_atoms / struct_conn / struct_ref_seq_dif / struct_site
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession ..._database_2.pdbx_DOI / _database_2.pdbx_database_accession / _struct_conn.pdbx_leaving_atom_flag / _struct_ref_seq_dif.details / _struct_site.pdbx_auth_asym_id / _struct_site.pdbx_auth_comp_id / _struct_site.pdbx_auth_seq_id
Revision 2.2Aug 30, 2023Group: Data collection / Refinement description
Category: chem_comp_atom / chem_comp_bond / pdbx_initial_refinement_model
Revision 2.3Mar 13, 2024Group: Source and taxonomy / Category: pdbx_entity_src_syn

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Estrogen receptor
B: Estrogen receptor
hetero molecules


Theoretical massNumber of molelcules
Total (without water)59,9734
Polymers59,0252
Non-polymers9472
Water5,405300
1


  • Idetical with deposited unit
  • defined by author&software
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area5140 Å2
ΔGint-16 kcal/mol
Surface area19950 Å2
MethodPISA
Unit cell
Length a, b, c (Å)101.588, 58.102, 87.950
Angle α, β, γ (deg.)90.000, 102.660, 90.000
Int Tables number5
Space group name H-MC121

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Components

#1: Protein Estrogen receptor / ER / Estradiol receptor / ER-alpha


Mass: 29512.742 Da / Num. of mol.: 2 / Fragment: STEROID-BINDING REGION, RESIDUES 298-554 / Mutation: L536S
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: ESR1, ESR, NR3A1 / Plasmid: pMCSG7 / Production host: Escherichia coli (E. coli) / Strain (production host): BL21(DE3) Rosetta / References: UniProt: P03372
#2: Chemical ChemComp-RAL / RALOXIFENE


Mass: 473.583 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: C28H27NO4S / Comment: medication*YM
#3: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 300 / Source method: isolated from a natural source / Formula: H2O

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.15 Å3/Da / Density % sol: 42.66 %
Crystal growTemperature: 298 K / Method: vapor diffusion, hanging drop / pH: 8
Details: pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K

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Data collection

DiffractionMean temperature: 100 K
Diffraction sourceSource: SYNCHROTRON / Site: APS / Beamline: 22-ID / Wavelength: 0.9764 Å
DetectorType: MARMOSAIC 300 mm CCD / Detector: CCD / Date: Mar 16, 2006 / Details: Rosenbaum-Rock vertical focusing mirror
RadiationMonochromator: Rosenbaum-Rock monochromator high-resolution double-crystal Si(220) sagittal focusing
Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.9764 Å / Relative weight: 1
ReflectionResolution: 1.7→20 Å / Num. all: 55230 / Num. obs: 55230 / % possible obs: 99.9 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 6.7 % / Biso Wilson estimate: 18.7 Å2 / Rmerge(I) obs: 0.066 / Rsym value: 0.066 / Χ2: 1.044 / Net I/σ(I): 22.3
Reflection shellResolution: 1.7→1.76 Å / Redundancy: 4.5 % / Rmerge(I) obs: 0.303 / Mean I/σ(I) obs: 5.5 / Num. unique all: 5450 / Rsym value: 0.303 / Χ2: 1.04 / % possible all: 99

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Phasing

PhasingMethod: molecular replacement

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Processing

Software
NameVersionClassificationNB
DENZOdata reduction
SCALEPACKdata scaling
PHASERphasing
REFMAC5.2.0019refinement
PDB_EXTRACT3data extraction
HKL-2000data collection
HKL-2000data reduction
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: PDB entry 1err

1err


Resolution: 1.7→15 Å / Cor.coef. Fo:Fc: 0.953 / Cor.coef. Fo:Fc free: 0.937 / SU B: 4.059 / SU ML: 0.063 / Isotropic thermal model: isotropic / Cross valid method: THROUGHOUT / σ(F): 0 / σ(I): 0 / ESU R: 0.109 / ESU R Free: 0.104 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
RfactorNum. reflection% reflectionSelection details
Rfree0.216 2795 5.1 %RANDOM
Rwork0.183 ---
obs0.184 55018 99.85 %-
all-55018 --
Solvent computationIon probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.4 Å / Solvent model: MASK
Displacement parametersBiso mean: 10.195 Å2
Baniso -1Baniso -2Baniso -3
1--0.07 Å20 Å2-0.04 Å2
2--0.05 Å20 Å2
3---0 Å2
Refinement stepCycle: LAST / Resolution: 1.7→15 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms3645 0 68 300 4013
Refine LS restraints
Refine-IDTypeDev idealDev ideal targetNumber
X-RAY DIFFRACTIONr_bond_refined_d0.0120.0213848
X-RAY DIFFRACTIONr_angle_refined_deg1.4642.0015224
X-RAY DIFFRACTIONr_dihedral_angle_1_deg8.385483
X-RAY DIFFRACTIONr_dihedral_angle_2_deg37.01224.408152
X-RAY DIFFRACTIONr_dihedral_angle_3_deg15.04515707
X-RAY DIFFRACTIONr_dihedral_angle_4_deg19.0981517
X-RAY DIFFRACTIONr_chiral_restr0.1780.2612
X-RAY DIFFRACTIONr_gen_planes_refined0.0060.022782
X-RAY DIFFRACTIONr_nbd_refined0.2330.21980
X-RAY DIFFRACTIONr_nbtor_refined0.3030.22663
X-RAY DIFFRACTIONr_xyhbond_nbd_refined0.3590.2234
X-RAY DIFFRACTIONr_symmetry_vdw_refined0.2610.261
X-RAY DIFFRACTIONr_symmetry_hbond_refined0.3770.210
X-RAY DIFFRACTIONr_mcbond_it0.7881.52411
X-RAY DIFFRACTIONr_mcangle_it1.16323773
X-RAY DIFFRACTIONr_scbond_it2.07231652
X-RAY DIFFRACTIONr_scangle_it3.0484.51437
LS refinement shellResolution: 1.7→1.744 Å / Total num. of bins used: 20
RfactorNum. reflection% reflection
Rfree0.277 187 -
Rwork0.216 3765 -
all-3952 -
obs-3765 98.33 %
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
16.8581-4.0746-3.56356.70411.09675.2675-0.1619-0.27180.25560.34030.1690.0911-0.2240.0276-0.00710.1063-0.0123-0.0325-0.06-0.05070.060631.280422.734726.9377
29.3602-5.1489-3.96533.30373.49465.33880.0846-0.08170.2818-0.01880.00470.0083-0.09780.024-0.08940.0222-0.0547-0.00290.0195-0.00090.067446.730812.205617.9388
313.36443.5036-8.893911.91463.70539.2332-0.5731-0.5272-0.70250.05560.2385-0.10080.59980.92040.33460.11370.07810.00850.02450.09970.184258.3455-0.61937.9476
427.02884.4752-4.691216.19261.846221.7994-0.24960.4026-1.2448-0.4273-0.0996-0.13371.17990.57950.34920.00310.02510.0001-0.08140.00740.091350.9422-8.108812.2682
53.4684-2.6632-2.48775.07043.43822.55610.0787-0.2202-0.15770.0261-0.0434-0.08420.07270.1252-0.03530.014-0.0329-0.01970.02540.02520.015648.72912.013520.8193
68.6958-2.40960.27986.79351.046711.25540.0726-0.29770.44430.0724-0.0127-0.3385-0.36510.5651-0.05980.0196-0.0673-0.00110.0391-0.02840.001941.302412.385428.8287
71.0231-0.4147-0.87820.60380.56013.6385-0.0492-0.11190.00180.05390.01750.0233-0.0184-0.0340.03170.0235-0.023-0.01040.0215-0.00590.019836.71275.722523.9792
84.12162.7521.77474.99792.91043.26930.00270.4961-0.3314-0.13350.0925-0.23460.11050.241-0.0952-0.0161-0.0039-0.00530.0256-0.0183-0.003145.39390.44535.7448
98.03873.1143-5.638921.0259-14.114126.93630.0796-0.2529-0.0654-1.4592-0.8988-0.62061.07181.50350.8192-0.00430.10960.0422-0.0580.03250.046543.2157-10.91447.834
107.0328-6.30180.217215.8915-0.90043.26070.03570.3604-0.27210.0066-0.15390.20020.2815-0.14690.11820.0504-0.0420.00090.0297-0.03570.01834.0277-1.75687.4929
1110.6679-5.6718-0.015321.64714.71814.3190.03470.4007-0.1617-0.4904-0.1436-0.1572-0.1288-0.15620.10890.0343-0.00550.02540.03440.0268-0.037129.797610.89746.5249
121.5684-0.3979-0.47863.8976-1.94915.82840.01540.05420.0954-0.0099-0.0624-0.0346-0.1799-0.00040.0470.0226-0.0211-0.00910.003-0.01780.044731.397511.965220.2603
132.5462-1.7492-3.66359.860310.267612.2094-0.0416-0.1279-0.07651.3637-0.20990.04092.0911-1.42360.25140.2248-0.08430.02490.13070.02210.003126.41282.100237.3685
142.1207-1.42211.33114.7582-7.268313.4868-0.0717-0.27860.14740.15780.12060.0905-0.1414-0.1777-0.04890.0415-0.02270.00160.0095-0.04950.053524.199315.053328.5101
159.91993.09164.991611.12270.761511.035-0.02130.27420.496-0.5094-0.27250.3581-0.4320.53390.29380.03250.0436-0.0302-0.02950.06060.04319.013823.118211.745
162.0393-1.58920.59127.5482-1.75811.85730.01580.22220.0418-0.1235-0.14450.1348-0.0508-0.07990.1287-0.0046-0.0102-0.0090.0133-0.00610.026320.880511.101814.0074
178.2039-2.1686-1.52148.06460.95573.34090.003-0.10370.1701-0.1166-0.06120.01120.0602-0.23420.05820.0307-0.0410.00150.0228-0.01180.052129.7143-2.550416.9912
1813.8285-10.0495-0.066512.5329-5.78466.50590.2859-0.0829-0.4994-0.0239-0.17430.19370.1719-0.1792-0.11160.0429-0.0437-0.0087-0.02120.03050.143137.7918-10.598919.6913
192.3838-2.70440.416516.83937.37214.5413-0.5185-0.4827-0.33421.11010.7429-0.12940.5280.4003-0.22450.07020.0393-0.00330.07710.09950.077944.7413-7.911428.3664
2010.053812.474117.21215.493121.580932.6210.33050.5288-0.4920.1516-0.0196-0.19230.08061.5326-0.3110.01620.0696-0.02980.35040.0186-0.137445.91585.83137.4501
2162.873-0.5079-58.591110.83921.723761.8183-0.68211.7516-1.47660.412-0.8311-0.10990.4094-0.61881.51320.04210.0709-0.12450.00340.04240.14963.93615.98073.2486
220.43910.67520.045620.1818-7.73693.82120.1338-0.08410.17710.1492-0.02820.8774-0.1381-0.2169-0.1057-0.0361-0.03110.01630.0244-0.05520.07841.6783-3.824517.1348
2343.2721-3.68061.47356.74580.006414.44140.3106-1.586-1.71831.1771-0.3112-0.25210.8682-0.04870.00060.1478-0.1666-0.1202-0.00840.17150.010312.6131-25.442329.5685
242.6326-0.71551.73546.0654-3.37023.37510.0794-0.1461-0.17580.1353-0.0925-0.0920.0278-0.05360.0131-0.004-0.05650.01070.0594-0.01790.03299.5217-17.166117.8895
254.7374-1.9964-1.6744.98710.73993.5157-0.04360.25260.1125-0.3829-0.01080.0532-0.1819-0.01180.05430.0536-0.0459-0.03150.0273-0.0132-0.022611.6236-6.79213.7917
261.7540.6846-0.64982.20320.46334.21690.1167-0.1299-0.03920.0823-0.18780.03120.0076-0.07960.07110.02-0.06050.00170.0532-0.01660.013714.7362-9.150619.5342
275.0281.1807-0.55256.0406-0.97493.27130.3402-0.56660.30130.7751-0.34280.1778-0.1562-0.1540.00260.0639-0.18240.04560.1514-0.0482-0.06719.8682-9.894533.9685
2829.14744.484512.93961.15480.322811.72980.4285-1.3765-0.39370.5097-0.50680.05120.2185-0.16130.07830.1023-0.2024-0.00820.13360.0384-0.143412.6381-16.778936.2973
291.3423-2.8849-6.7116.970213.220933.6753-0.4039-1.5538-0.27190.6512-0.19160.39462.6113-0.54530.59550.0579-0.1157-0.05320.09260.2718-0.044523.4557-20.313333.9121
308.5667-9.2814.099620.6826-5.37835.52710.2555-0.21540.15050.7156-0.53-0.3664-0.13520.64280.27450.0217-0.159-0.01370.17870.0109-0.09120.295-8.404532.7761
3113.9953-6.99969.517215.0491-5.33614.702-0.0606-0.44750.23560.9599-0.02730.0835-0.43380.2060.08790.0795-0.1170.12390.0735-0.1441-0.067711.2063.321431.1077
322.7389-0.6145-3.16274.68881.58327.98030.1338-0.1220.08210.0564-0.14780.2065-0.00130.03390.014-0.0067-0.0529-0.00870.025-0.03390.048511.9501-0.333216.7832
3311.23542.446310.871415.852-4.691916.819-0.05520.94990.2224-0.6164-0.253-0.8444-0.14611.55860.30820.0605-0.08320.12980.11960.01490.004120.25930.95471.252
345.8476-2.1771-6.51495.57137.287314.3702-0.07840.16510.2504-0.1438-0.0980.25680.0191-0.33190.1763-0.031-0.0034-0.03480.0122-0.00130.068810.94198.185611.5366
3523.54660.76565.060.98760.53938.3409-0.3419-0.08610.3124-0.07710.09750.1427-0.2742-0.61560.2444-0.03520.0360.0380.0054-0.11470.1147.751518.028323.9809
364.0524-4.7037-0.103310.80251.32721.63550.0445-0.09110.06690.1482-0.14820.14670.0413-0.17480.10370.0084-0.04340.02530.0085-0.03330.040416.6899.312123.5393
375.2851-0.76981.154213.59350.63133.42250.0689-0.1504-0.00560.1085-0.0753-0.0934-0.08460.04190.00650.0354-0.064-0.00440.05630.02530.023524.2587-7.116523.0092
3815.8674-4.9714-5.129518.1506-3.832214.25440.1257-0.0943-0.39620.2269-0.1295-0.52890.17650.13460.0038-0.0148-0.0471-0.0625-0.040.06080.083727.047-18.645922.3016
3917.14691.535543.038242.4295-1.2852108.64891.01980.5566-0.6938-0.7845-3.0535-0.01923.4188-0.42682.03370.10550.01440.0183-0.0034-0.08840.441424.8204-25.735119.7896
405.0682-2.31722.12864.5818-0.93814.95990.0519-0.0332-0.2053-0.2071-0.00320.1381-0.1342-0.0384-0.04870.0271-0.01260.00140.0415-0.0406-0.010412.6976-18.32744.9869
Refinement TLS group
IDRefine-IDRefine TLS-IDAuth asym-IDLabel asym-IDAuth seq-IDLabel seq-ID
1X-RAY DIFFRACTION1AA306 - 32010 - 24
2X-RAY DIFFRACTION2AA321 - 32925 - 33
3X-RAY DIFFRACTION3AA330 - 33834 - 42
4X-RAY DIFFRACTION4AA340 - 34344 - 47
5X-RAY DIFFRACTION5AA344 - 35748 - 61
6X-RAY DIFFRACTION6AA358 - 36362 - 67
7X-RAY DIFFRACTION7AA364 - 40068 - 104
8X-RAY DIFFRACTION8AA401 - 414105 - 118
9X-RAY DIFFRACTION9AA415 - 421119 - 125
10X-RAY DIFFRACTION10AA422 - 434126 - 138
11X-RAY DIFFRACTION11AA435 - 440139 - 144
12X-RAY DIFFRACTION12AA441 - 455145 - 159
13X-RAY DIFFRACTION13AA456 - 468160 - 172
14X-RAY DIFFRACTION14AA469 - 491173 - 195
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