The biological assembly is a decamer consisting of the five copies of the N protein in the asymmetric unit plus an additional five copies created by rotation of the contents of the asymmetric unit by 180 degrees around the c-axis.
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Components
#1: Protein
Nucleocapsidprotein / Protein N / Nucleoprotein / NP
Mass: 47431.883 Da / Num. of mol.: 5 / Mutation: S290W Source method: isolated from a genetically manipulated source Source: (gene. exp.) Vesicular stomatitis Indiana virus / Genus: Vesiculovirus / Strain: Indiana / Gene: N / Plasmid: pET16b / Species (production host): Escherichia coli / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21(DE3) / References: UniProt: P03521, UniProt: A6H4P1*PLUS
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
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Sample preparation
Crystal
Density Matthews: 3.1 Å3/Da / Density % sol: 60.36 %
Crystal grow
Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 4.5 Details: 5.5 % PEG 3350, 200 mM sodium chloride, 100mM sodium acetate, pH 4.5, vapor diffusion, hanging drop, temperature 293.0K
Resolution: 2.8→41.62 Å / Cor.coef. Fo:Fc: 0.878 / Cor.coef. Fo:Fc free: 0.839 / SU B: 35.983 / SU ML: 0.326 / Cross valid method: THROUGHOUT / σ(F): 0 / ESU R Free: 0.508 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
Rfactor
Num. reflection
% reflection
Selection details
Rfree
0.286
2796
5.1 %
RANDOM
Rwork
0.243
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obs
0.246
54826
74.41 %
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Solvent computation
Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK
Displacement parameters
Biso mean: 34.198 Å2
Baniso -1
Baniso -2
Baniso -3
1-
4.59 Å2
0 Å2
0 Å2
2-
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-1.74 Å2
0 Å2
3-
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-2.86 Å2
Refinement step
Cycle: LAST / Resolution: 2.8→41.62 Å
Protein
Nucleic acid
Ligand
Solvent
Total
Num. atoms
16675
0
0
0
16675
Refine LS restraints
Refine-ID
Type
Dev ideal
Dev ideal target
Number
X-RAY DIFFRACTION
r_bond_refined_d
0.006
0.022
17065
X-RAY DIFFRACTION
r_angle_refined_deg
0.953
1.963
23120
X-RAY DIFFRACTION
r_dihedral_angle_1_deg
4.475
5
2100
X-RAY DIFFRACTION
r_dihedral_angle_2_deg
37.78
24.052
765
X-RAY DIFFRACTION
r_dihedral_angle_3_deg
15.887
15
2945
X-RAY DIFFRACTION
r_dihedral_angle_4_deg
12.559
15
100
X-RAY DIFFRACTION
r_chiral_restr
0.069
0.2
2510
X-RAY DIFFRACTION
r_gen_planes_refined
0.002
0.02
12945
X-RAY DIFFRACTION
r_nbd_refined
0.182
0.2
7536
X-RAY DIFFRACTION
r_nbtor_refined
0.297
0.2
11889
X-RAY DIFFRACTION
r_xyhbond_nbd_refined
0.119
0.2
363
X-RAY DIFFRACTION
r_symmetry_vdw_refined
0.127
0.2
155
X-RAY DIFFRACTION
r_symmetry_hbond_refined
0.102
0.2
15
X-RAY DIFFRACTION
r_mcbond_it
1.126
1.5
10729
X-RAY DIFFRACTION
r_mcangle_it
2.03
2
16900
X-RAY DIFFRACTION
r_scbond_it
0.879
3
7294
X-RAY DIFFRACTION
r_scangle_it
1.379
4.5
6220
LS refinement shell
Resolution: 2.8→2.872 Å / Total num. of bins used: 20