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- PDB-2qre: Crystal structure of the adenylate sensor from AMP-activated prot... -
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Open data
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Basic information
Entry | Database: PDB / ID: 2qre | ||||||
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Title | Crystal structure of the adenylate sensor from AMP-activated protein kinase in complex with 5-aminoimidazole-4-carboxamide 1-beta-D-ribofuranotide (ZMP) | ||||||
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![]() | TRANSFERASE / AMPK / 5-aminoimidazole-4-carboxamide 1-beta-D-ribofuranotide / ZMP / AICAR phosphate / ATP-binding / Kinase / Nucleotide-binding / Serine/threonine-protein kinase / CBS domain | ||||||
Function / homology | ![]() positive regulation of flocculation / positive regulation of ascus development / positive regulation of cell cycle switching, mitotic to meiotic cell cycle / AMPK inhibits chREBP transcriptional activation activity / Carnitine metabolism / induction of conjugation with cellular fusion / Energy dependent regulation of mTOR by LKB1-AMPK / TP53 Regulates Metabolic Genes / Macroautophagy / SREBP signaling pathway ...positive regulation of flocculation / positive regulation of ascus development / positive regulation of cell cycle switching, mitotic to meiotic cell cycle / AMPK inhibits chREBP transcriptional activation activity / Carnitine metabolism / induction of conjugation with cellular fusion / Energy dependent regulation of mTOR by LKB1-AMPK / TP53 Regulates Metabolic Genes / Macroautophagy / SREBP signaling pathway / mitotic spindle pole body / CAMKK-AMPK signaling cascade / nucleotide-activated protein kinase complex / protein kinase regulator activity / protein kinase activator activity / AMP binding / negative regulation of cytoplasmic translation / negative regulation of TORC1 signaling / ADP binding / non-specific serine/threonine protein kinase / protein serine kinase activity / protein serine/threonine kinase activity / regulation of transcription by RNA polymerase II / protein kinase binding / signal transduction / positive regulation of transcription by RNA polymerase II / ATP binding / nucleus / plasma membrane / cytosol / cytoplasm Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Jin, X. / Townley, R. / Shapiro, L. | ||||||
![]() | ![]() Title: Structural Insight into AMPK Regulation: ADP Comes into Play. Authors: Jin, X. / Townley, R. / Shapiro, L. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 209.8 KB | Display | ![]() |
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PDB format | ![]() | 167.7 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 2qr1C ![]() 2qrcC ![]() 2qrdC ![]() 200yS C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Details | The biological unit is a heterotrimer (There are two such trimers: A+B+G and C+D+E in the asymmetric unit). The dimer of these heterotrimers is also physiologically relevant. |
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Components
#1: Protein | Mass: 15903.413 Da / Num. of mol.: 2 / Fragment: C-terminal residues:440-576 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Gene: ssp2 / Plasmid: PSMT3 / Species (production host): Escherichia coli / Production host: ![]() ![]() References: UniProt: O74536, non-specific serine/threonine protein kinase #2: Protein | Mass: 10865.345 Da / Num. of mol.: 2 / Fragment: C-terminal residues:203-298 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Plasmid: PET-DUET-1 / Species (production host): Escherichia coli / Production host: ![]() ![]() #3: Protein | Mass: 37436.070 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Plasmid: PET-DUET-1 / Species (production host): Escherichia coli / Production host: ![]() ![]() #4: Chemical | #5: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.3 Å3/Da / Density % sol: 46.63 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 5.5 Details: 6-10% PEG 3350, 0.1M sodium citrate, pH 5.5, 2mM ZMP, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 315 / Detector: CCD / Date: Feb 15, 2007 |
Radiation | Monochromator: Si 111 CHANNEL / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97926 Å / Relative weight: 1 |
Reflection | Resolution: 3→50 Å / Num. all: 21790 / Num. obs: 20826 / % possible obs: 89.3 % / Observed criterion σ(F): 1 / Observed criterion σ(I): 1 / Redundancy: 3.9 % / Rmerge(I) obs: 0.18 / Net I/σ(I): 4.6 |
Reflection shell | Resolution: 3→3.11 Å / Redundancy: 3.3 % / Rmerge(I) obs: 0.63 / Mean I/σ(I) obs: 1.5 / Num. unique all: 2232 / % possible all: 96 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 200y Resolution: 3.01→41.34 Å / Cor.coef. Fo:Fc: 0.894 / Cor.coef. Fo:Fc free: 0.847 / SU B: 46.792 / SU ML: 0.405 / TLS residual ADP flag: LIKELY RESIDUAL / Cross valid method: THROUGHOUT / σ(F): 1 / σ(I): 1 / ESU R Free: 0.61 / Stereochemistry target values: MAXIMUM LIKELIHOOD
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.4 Å / Solvent model: MASK | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 89.957 Å2
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Refinement step | Cycle: LAST / Resolution: 3.01→41.34 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 3.01→3.089 Å / Total num. of bins used: 20
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Refinement TLS params. | Method: refined / Refine-ID: X-RAY DIFFRACTION
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Refinement TLS group |
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