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- PDB-7m7a: Legionella pneumophila MavQ lipid kinase -

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Basic information

Entry
Database: PDB / ID: 7m7a
TitleLegionella pneumophila MavQ lipid kinase
ComponentsPhosphoinositide 3-kinase MavQ
KeywordsTRANSFERASE
Function / homologyADENOSINE-5'-DIPHOSPHATE / Uncharacterized protein
Function and homology information
Biological speciesLegionella pneumophila subsp. pneumophila (bacteria)
MethodX-RAY DIFFRACTION / SYNCHROTRON / SAD / Resolution: 3.2 Å
AuthorsTomchick, D.R. / Tagliabracci, V.S. / Hsieh, T.S. / Lopez, V.A.
Funding support United States, Poland, 10items
OrganizationGrant numberCountry
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)DP2GM137419 United States
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)R01GM113079 United States
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)T32GM008203-29 United States
National Institutes of Health/National Heart, Lung, and Blood Institute (NIH/NHLBI)F30HL143859-01 United States
National Institutes of Health/Office of the DirectorS10OD025018 United States
Robert A. Welch FoundationI-1911 United States
Robert A. Welch FoundationI-1789 United States
Cancer Prevention and Research Institute of Texas (CPRIT)RP170674 United States
National Center for Research and Development (Poland)PPN/BEK/2018/1/00431 Poland
National Institutes of Health/Office of the DirectorS10OD021685 United States
CitationJournal: Science / Year: 2021
Title: Dynamic remodeling of host membranes by self-organizing bacterial effectors.
Authors: Hsieh, T.S. / Lopez, V.A. / Black, M.H. / Osinski, A. / Pawlowski, K. / Tomchick, D.R. / Liou, J. / Tagliabracci, V.S.
History
DepositionMar 27, 2021Deposition site: RCSB / Processing site: RCSB
Revision 1.0Apr 28, 2021Provider: repository / Type: Initial release
Revision 1.1May 12, 2021Group: Database references / Category: citation / citation_author
Item: _citation.pdbx_database_id_PubMed / _citation.title / _citation_author.identifier_ORCID
Revision 1.2Jun 9, 2021Group: Database references / Category: citation
Item: _citation.journal_volume / _citation.page_first / _citation.page_last
Revision 1.3May 22, 2024Group: Data collection / Database references / Category: chem_comp_atom / chem_comp_bond / database_2
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Phosphoinositide 3-kinase MavQ
B: Phosphoinositide 3-kinase MavQ
C: Phosphoinositide 3-kinase MavQ
D: Phosphoinositide 3-kinase MavQ
hetero molecules


Theoretical massNumber of molelcules
Total (without water)275,72312
Polymers273,9174
Non-polymers1,8068
Water00
1
A: Phosphoinositide 3-kinase MavQ
hetero molecules


Theoretical massNumber of molelcules
Total (without water)68,9313
Polymers68,4791
Non-polymers4522
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
MethodPISA
2
B: Phosphoinositide 3-kinase MavQ
hetero molecules


Theoretical massNumber of molelcules
Total (without water)68,9313
Polymers68,4791
Non-polymers4522
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
MethodPISA
3
C: Phosphoinositide 3-kinase MavQ
hetero molecules


Theoretical massNumber of molelcules
Total (without water)68,9313
Polymers68,4791
Non-polymers4522
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
MethodPISA
4
D: Phosphoinositide 3-kinase MavQ
hetero molecules


Theoretical massNumber of molelcules
Total (without water)68,9313
Polymers68,4791
Non-polymers4522
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
MethodPISA
Unit cell
Length a, b, c (Å)69.399, 210.235, 499.861
Angle α, β, γ (deg.)90.000, 90.000, 90.000
Int Tables number20
Space group name H-MC2221
Space group name HallC2c2
Symmetry operation#1: x,y,z
#2: x,-y,-z
#3: -x,y,-z+1/2
#4: -x,-y,z+1/2
#5: x+1/2,y+1/2,z
#6: x+1/2,-y+1/2,-z
#7: -x+1/2,y+1/2,-z+1/2
#8: -x+1/2,-y+1/2,z+1/2
Noncrystallographic symmetry (NCS)NCS domain:
IDEns-IDDetails
d_1ens_1(chain "A" and (resid 2 through 15 or resid 29...
d_2ens_1(chain "B" and (resid 2 through 576 or resid 701 through 702))
d_3ens_1(chain "C" and (resid 2 through 15 or resid 29...
d_4ens_1(chain "D" and (resid 2 through 48 or resid 54...

NCS domain segments:
Dom-IDComponent-IDEns-IDBeg label comp-IDEnd label comp-IDLabel asym-IDLabel seq-ID
d_11ens_1GLYPHEA1 - 14
d_12ens_1THRLEUA20 - 39
d_13ens_1TYRMETA45 - 163
d_14ens_1VALGLNA166 - 195
d_15ens_1TYRVALA198 - 390
d_16ens_1HISTHRA394 - 537
d_17ens_1ADPADPB
d_21ens_1GLYTHRD1 - 520
d_22ens_1ADPADPE
d_31ens_1GLYPHEG1 - 14
d_32ens_1THRLEUG17 - 36
d_33ens_1TYRMETG42 - 160
d_34ens_1VALGLNG164 - 193
d_35ens_1TYRVALG195 - 387
d_36ens_1HISTHRG391 - 534
d_37ens_1ADPADPH
d_41ens_1GLYLEUJ1 - 34
d_42ens_1TYRMETJ37 - 155
d_43ens_1VALGLNJ159 - 188
d_44ens_1TYRVALJ190 - 382
d_45ens_1HISTHRJ386 - 529
d_46ens_1ADPADPK

NCS oper:
IDCodeMatrixVector
1given(0.0439845537993, -0.98605493317, 0.16050242303), (-0.98491040732, -0.0697168814005, -0.158401534082), (0.167382342494, -0.151113286045, -0.974242231789)27.2102100037, 50.2256193289, 126.774710276
2given(0.965705040742, 0.0369804120381, 0.25699459802), (-0.0453210221846, 0.998618125506, 0.0266053445399), (-0.255655587137, -0.037340173211, 0.966046547652)-33.0950466373, 49.5104241072, 4.88953427156
3given(-0.00305634303733, -0.98961271635, -0.143726582112), (-0.979056562149, 0.0322193229313, -0.201022793095), (0.203565485486, 0.140102058758, -0.968985297231)31.1065770769, 100.838476981, 116.581977761

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Components

#1: Protein
Phosphoinositide 3-kinase MavQ


Mass: 68479.328 Da / Num. of mol.: 4 / Fragment: MavQ, UNP residues 1-580
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Legionella pneumophila subsp. pneumophila (bacteria)
Strain: Philadelphia 1 / ATCC 33152 / DSM 7513 / Gene: lpg2975 / Plasmid: ppSUMO / Production host: Escherichia coli BL21(DE3) (bacteria) / Variant (production host): Rosetta
References: UniProt: Q5ZRA8, phosphatidylinositol-3-phosphatase
#2: Chemical
ChemComp-ADP / ADENOSINE-5'-DIPHOSPHATE


Mass: 427.201 Da / Num. of mol.: 4 / Source method: obtained synthetically / Formula: C10H15N5O10P2 / Feature type: SUBJECT OF INVESTIGATION / Comment: ADP, energy-carrying molecule*YM
#3: Chemical
ChemComp-MG / MAGNESIUM ION


Mass: 24.305 Da / Num. of mol.: 4 / Source method: obtained synthetically / Formula: Mg / Feature type: SUBJECT OF INVESTIGATION
Has ligand of interestY
Sequence detailsSEFELRRQAS is from the purification tag

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 3.33 Å3/Da / Density % sol: 63.07 % / Mosaicity: 0.359 °
Crystal growTemperature: 293 K / Method: vapor diffusion, sitting drop / pH: 7
Details: 10% (w/v) PEG 3350, 0.05 M sodium chloride, 0.1 M Hepes, 5 mM magnesium chloride, 5 mM AMP-PNP, 35% ethylene glycol

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: APS / Beamline: 19-ID / Wavelength: 0.97951 Å
DetectorType: DECTRIS PILATUS3 X 6M / Detector: PIXEL / Date: Apr 11, 2018 / Details: monochromator
RadiationMonochromator: SAGITALLY FOCUSED Si(111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.97951 Å / Relative weight: 1
ReflectionResolution: 3.2→50 Å / Num. obs: 60636 / % possible obs: 98.8 % / Redundancy: 30.4 % / Rmerge(I) obs: 0.168 / Rpim(I) all: 0.029 / Rrim(I) all: 0.171 / Χ2: 1.072 / Net I/σ(I): 4.5 / Num. measured all: 1844250
Reflection shell

Diffraction-ID: 1

Resolution (Å)Redundancy (%)Rmerge(I) obsNum. unique obsCC1/2Rpim(I) allRrim(I) allΧ2% possible all
3.2-3.2613.81.00327380.7440.2441.041.05690.5
3.26-3.3115.50.97428890.7540.2251.0051.04794.6
3.31-3.38180.90629020.9060.1990.9311.04297
3.38-3.4519.90.83229830.9220.1730.8521.06897.1
3.45-3.5222.70.79629380.9470.1590.8131.06798.4
3.52-3.624.80.64129850.9830.1230.6541.06498.6
3.6-3.6926.60.60330520.9860.1130.6141.10799.5
3.69-3.7928.50.52630110.9910.0960.5351.103100
3.79-3.9129.60.41230540.9950.0740.4191.14100
3.91-4.03290.38429990.9950.0690.3911.143100
4.03-4.1833.70.34930410.9960.060.3541.145100
4.18-4.34350.30331150.9970.0510.3071.181100
4.34-4.54370.26329990.9980.0440.2661.186100
4.54-4.7838.10.21830880.9980.0360.2211.194100
4.78-5.0838.50.230660.9990.0320.2021.175100
5.08-5.4736.50.20130780.9990.0330.2041.136100
5.47-6.0241.50.19530890.9980.0310.1971.082100
6.02-6.8940.30.16731410.9980.0270.1691.029100
6.89-8.67370.10831610.9990.0180.1090.9299.8
8.67-50370.0733070.9990.0120.0710.614100

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Processing

Software
NameVersionClassification
PHENIX1.19.2-4158refinement
HKL-3000data scaling
HKL-3000data reduction
PDB_EXTRACT3.27data extraction
SHELXDphasing
MLPHAREphasing
PARROTphasing
BUCCANEERmodel building
Cootmodel building
RefinementMethod to determine structure: SAD / Resolution: 3.2→49.31 Å / SU ML: 0.3784 / Cross valid method: FREE R-VALUE / σ(F): 1.34 / Phase error: 28.4635
Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
RfactorNum. reflection% reflection
Rfree0.2834 1995 4.03 %
Rwork0.2395 47478 -
obs0.2412 49473 80.86 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso mean: 58.58 Å2
Refinement stepCycle: LAST / Resolution: 3.2→49.31 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms17540 0 112 0 17652
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.002318082
X-RAY DIFFRACTIONf_angle_d0.577824497
X-RAY DIFFRACTIONf_chiral_restr0.03682659
X-RAY DIFFRACTIONf_plane_restr0.00393130
X-RAY DIFFRACTIONf_dihedral_angle_d13.57826706
Refine LS restraints NCS
Ens-IDDom-IDAuth asym-IDRefine-IDTypeRms dev position (Å)
ens_1d_2AX-RAY DIFFRACTIONTorsion NCS1.02404002821
ens_1d_3AX-RAY DIFFRACTIONTorsion NCS0.999764838008
ens_1d_4AX-RAY DIFFRACTIONTorsion NCS1.01253664508
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
3.2-3.280.3453560.3271322X-RAY DIFFRACTION31.93
3.28-3.370.4103660.32691572X-RAY DIFFRACTION38.13
3.37-3.470.3877770.30731827X-RAY DIFFRACTION44.02
3.47-3.580.3653960.28782312X-RAY DIFFRACTION56.04
3.58-3.710.29761290.2813052X-RAY DIFFRACTION73.41
3.71-3.860.32961540.25893676X-RAY DIFFRACTION88.7
3.86-4.030.29771720.24374083X-RAY DIFFRACTION98.59
4.03-4.240.26311760.23054175X-RAY DIFFRACTION99.91
4.24-4.510.25541740.20444162X-RAY DIFFRACTION99.91
4.51-4.860.25191760.19464184X-RAY DIFFRACTION99.93
4.86-5.350.26151780.20984234X-RAY DIFFRACTION99.95
5.35-6.120.2891770.25574223X-RAY DIFFRACTION100
6.12-7.70.34131790.2614266X-RAY DIFFRACTION99.84
7.7-49.310.22761850.22664390X-RAY DIFFRACTION98.39
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL112)L122)L132)L222)L232)L332)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T112)T122)T132)T222)T232)T332)Origin x (Å)Origin y (Å)Origin z (Å)
10.7266861097250.429640997709-0.7480710059371.26978575783-0.3767683138281.66858505737-0.0427324370593-0.372095039914-0.1795475100770.501331838416-0.051668532921-0.1991385956350.2179184039080.1366298051630.05559430955120.732161085493-0.0268165296917-0.03144556543740.692783911037-0.0439438424320.22999348439411.941279882118.8537123162118.597672806
20.9364051287570.00509093715466-0.3273008884710.567433405112-0.1858834864871.23131688135-0.00318724543657-0.22507093775-0.2563831480540.2653393772110.0135935514548-0.3417156531230.4636319777230.653781465377-0.01496270349320.4644361258910.117677001342-0.03548569212760.623360427004-0.03304071902280.27598502569323.376432695214.9605811062103.586551308
30.0901117752595-0.267258238215-0.07590028271320.925092540943-0.135735272421.18498136762-0.0647267993703-0.3839990165950.2459588026950.4269056843640.144134320109-0.196323198983-0.3731214585910.402950721859-0.1498008632060.413869426256-0.1287120565310.08908115619860.576094555146-0.2323636591040.27010452600116.869593341635.3482064029110.00665153
40.6488606002040.316245659528-0.2690740237630.906524871629-0.4386685671070.4491402151150.0596308310219-0.04378996281620.01111625033060.06373113258660.10186582910.0153186024026-0.007833436080030.1352555542920.07061973353860.159672102263-0.2279582604510.08052130603110.269805674099-0.0610591770940.13614924822112.789044025727.734199219583.900149321
50.9860203874460.654867039941-0.008206498049070.9911806425430.0007912605022140.0259063898661-0.0511636282210.1641124796290.0420130156115-0.1917359738740.16855315604-0.0960537806383-0.04578084859970.09121855612930.01508345339090.336997138896-0.3162748747390.02584104338250.513017300251-0.2033607599310.39425794849923.195251493350.562290442272.4139425767
60.6332167114030.313497469954-0.3106497630620.547018284232-0.2265059456590.3938337888510.0521132142709-0.02268016872080.0161876764255-0.06707246878670.08422191686090.0477180337783-0.00432620054508-0.009664317484480.02959619231810.23723560807-0.3757415361190.05967679426920.305453355054-0.01217326986180.11008156943111.817563775936.546014018168.5352424293
70.713955319791-0.0219688443197-0.4714624034440.7715719580961.005792922541.58647434061-0.002946444866110.362682589878-0.105364075718-0.0550886533892-0.00440714955806-0.01616739502560.05809299275490.094535235342-0.02244756123351.24454106091-0.1260584896110.01360629352460.702081570648-0.128431379060.28301602404828.46240770319.273864892311.3587666913
81.52905325303-0.764134755779-0.3267353312060.560654142296-0.3286627962481.40572251536-0.2015591580.416638884911-0.581583279595-0.1535713932810.00889414272841-0.1589885315510.4221653990950.07015973489740.08437222679821.10447009326-0.07301690405420.117148345950.430609786993-0.05934127745840.48119273985331.97755394099.4803219240727.2814128667
91.0308519784-0.02946784607680.03713954585090.175952540310.1133166020510.449102735544-0.07928354022650.368914952331-0.373022869932-0.462748926880.0317244232458-0.04142764893940.626832248015-0.152226412264-0.06571534234351.06035922089-0.357565881186-0.0683065310530.3895063811540.0102545736460.26203456316219.192103801514.766760228728.9452674892
101.089794735720.782437536196-0.2290552140751.03876414199-0.1033830552520.1055673928520.0443508856713-0.0793723805825-0.064605382179-0.0580821129938-0.015973815880.2763412940150.149020225343-0.2317518573520.0700640798260.601099720552-0.541129708522-0.1632977899980.5069599150890.06207737704440.34023710119-3.2766040597216.216174754550.4798165122
111.052920193410.540679431624-0.05145531495161.10795907302-0.1190651382931.644876072010.004323417756330.07216235473280.147188979917-0.07209050364010.1060512412140.1527950280260.0676476270211-0.209977536403-0.1058060633530.363120085317-0.358511140433-0.04222422178540.4130312934080.09796094061470.342193725009-2.8905811844526.349782706354.6020840114
121.315222242990.828738401815-0.5958121464370.853680871153-0.4206154892390.5718195230090.124780302564-0.195494970992-0.1789216977180.137813808427-0.0525824306801-0.06625083735620.02955076992290.03072395917810.00843771404610.334454226972-0.33821599544-0.09769184542590.3087034192710.01372037509230.21995465786310.730780781823.57689113559.3704729291
130.710029641108-0.226396982914-0.4145792882880.308287953390.1420786746650.600538744097-0.189615111671-0.199145627018-0.1478258858530.366412021612-0.00480974149605-0.07008302206830.2717579040640.2393654117380.1127679512061.152125104630.0565122327982-0.006284771133360.473824419754-0.03438811605880.27564129214310.808308173568.2495626764113.819922469
141.13665025652-0.550427494574-0.21617679214.26859115327-1.448768266860.64399231604-0.029978588567-0.100735933076-0.2372079983630.38952668694-0.0703963891637-0.3129059892780.2652141784670.1955003674860.05778619693040.8671543982520.238935330112-0.02551204378470.497462519596-0.02427275270030.25146739554214.824592688161.820509359596.0375305923
150.445592621256-0.2191149210530.1744851426330.7666168618360.5649806689031.082427179430.062633544768-0.280686223287-0.138647286990.2582997815050.0482739256633-0.3812495062860.09789150281590.376781815641-0.08077495267610.5659249188740.104480657376-0.1288101747020.5402425461980.09182505580710.38417685276620.472119975173.424363770296.8574739212
160.574187705290.181573969767-0.432402245550.629661939406-0.04352156593070.730267271046-0.0741615040741-0.1245675385020.2180328202550.302126004827-0.0832918543067-0.1402514664280.07684495309050.2895175226570.0784804034140.385521567627-0.326295289968-0.04092420482660.2618727463860.1025224091240.237507918028.4255947554379.232519575292.2368126929
170.1907410030370.143297758661-0.2392741246450.675602240206-0.7286363074360.830764004996-0.1547959895350.3133959952650.36536992375-0.166017964870.1194984074610.133283934937-0.1942793778780.03018466859120.0569214931630.522359066533-0.341746992483-0.2400042374510.5160406204160.162135548010.6798227879359.94839737982100.9446633766.0467037778
180.5457018848370.136378008656-0.07778997394880.318226630876-0.004469996574170.284421062362-0.08911256224360.04321632413040.100169375716-0.0182095326769-0.0992594126490.283778810232-0.0563197460509-0.0540149728956-0.05015642326990.273194201812-0.426360557862-0.1672899653030.3642620506730.1054156929920.401363735269-2.7817716721687.078990201166.7085469961
190.385273686237-0.106093089553-0.489221958110.473197802149-0.2133816702330.893233959557-0.06227363727850.3170352936110.0279646916487-0.310636413277-0.1060575110630.0589305792717-0.0877172183652-0.06387725561370.1087742858331.0198670418-0.312177034162-0.1103617036070.8112579192390.002519835092210.331765916918-5.8716646778467.28242696296.51679826705
200.7579938432850.0717769799510.0187811210580.8427388084610.5139365919640.67030865176-0.02187268246470.271484411301-0.390131271764-0.1057660711180.0317429229879-0.1659552764570.6949929220960.183600248041-0.01111054758451.14460304561-0.176019310377-0.0835292100590.6912547793530.08427893233660.4112318215722.6755281947556.337517848925.6537191585
211.27442203333-0.1392744816720.3212898761760.6233060412390.288287684010.68168959034-0.01104706676450.416082986277-0.097340364299-0.290742336789-0.06603964000250.241948247520.305233946225-0.30027909884-0.06365557420120.679149505261-0.469791447527-0.2403209412710.7261020820770.1459817111590.275339525703-12.471306731867.365671261725.1467671881
220.6882469119750.373016982429-0.407638043720.560881736068-0.233972528780.243056503288-0.0107101977836-0.10586025877-0.06314013121690.0227957636796-0.02010674380440.363862540290.114578247407-0.204763699986-0.004035647018710.53537829354-0.55786337804-0.07286661379880.6521027184070.07967468432030.477086300438-23.786191362168.000182402854.8232730525
230.3808861424260.234468909066-0.1311412253850.326783073489-0.1088122904520.365565232538-0.0132265108087-0.02351079343030.1943812596970.0214288939845-0.04415605194560.2011863989990.00811211125281-0.0577979690996-0.04070133330850.365126955849-0.494517701762-0.1696735378480.4475300453860.07872102833510.342033885746-15.147610322176.718376757357.7733265355
Refinement TLS group

Refine-ID: X-RAY DIFFRACTION

IDRefine TLS-IDSelection detailsAuth asym-IDLabel asym-IDAuth seq-IDLabel seq-ID
11chain 'A' and (resid 2 through 76 )AA2 - 761 - 67
22chain 'A' and (resid 77 through 265 )AA77 - 26568 - 226
33chain 'A' and (resid 266 through 338 )AA266 - 338227 - 299
44chain 'A' and (resid 339 through 429 )AA339 - 429300 - 390
55chain 'A' and (resid 430 through 478 )AA430 - 478391 - 439
66chain 'A' and (resid 479 through 577 )AA479 - 577440 - 538
77chain 'B' and (resid 2 through 106 )BD2 - 1061 - 87
88chain 'B' and (resid 107 through 189 )BD107 - 18988 - 157
99chain 'B' and (resid 190 through 391 )BD190 - 391158 - 338
1010chain 'B' and (resid 392 through 478 )BD392 - 478339 - 422
1111chain 'B' and (resid 479 through 534 )BD479 - 534423 - 478
1212chain 'B' and (resid 535 through 578 )BD535 - 578479 - 522
1313chain 'C' and (resid 2 through 116 )CG2 - 1161 - 104
1414chain 'C' and (resid 117 through 152 )CG117 - 152105 - 140
1515chain 'C' and (resid 153 through 209 )CG153 - 209141 - 187
1616chain 'C' and (resid 210 through 427 )CG210 - 427188 - 385
1717chain 'C' and (resid 428 through 478 )CG428 - 478386 - 436
1818chain 'C' and (resid 479 through 578 )CG479 - 578437 - 536
1919chain 'D' and (resid 2 through 96 )DJ2 - 961 - 79
2020chain 'D' and (resid 97 through 265 )DJ97 - 26580 - 218
2121chain 'D' and (resid 266 through 391 )DJ266 - 391219 - 344
2222chain 'D' and (resid 392 through 478 )DJ392 - 478345 - 431
2323chain 'D' and (resid 479 through 576 )DJ479 - 576432 - 529

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