structures with the lowest energy, no restraint violation, consistent with residual dipolar couplings
代表モデル
モデル #1
lowest energy
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要素
#1: RNA鎖
HumantelomeraseRNACR7terminalhairpinloop
分子量: 7716.614 Da / 分子数: 1 / 由来タイプ: 合成 詳細: RNA is synthesized using in vitro transcription with T7 RNA polymerase and a partially double-stranded DNA template
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実験情報
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実験
実験
手法: 溶液NMR
NMR実験
Conditions-ID
Experiment-ID
Solution-ID
タイプ
1
1
1
2D NOESY (11echo and WATERGATE)
2
2
1
2D NOESY, 2D TOCSY, Natural abundance 2D 13C HSQC
1
3
2
2D 15N-HMQC, 2D 15N-CPMG-NOESY, 2D JNN-HNN-COSY
2
4
2
2D 13C-HSQC, 2D (H)CCH-COCSY, 3D (H)CCH-TOCSY, 3D NOESY-HMQC, 2D 31P spin echo difference (H)CCH/HSQC/HMQC, 2D CT-CE-HSQC
手法: simulated annealing / ソフトェア番号: 1 詳細: Structures are based on 526 NOE-derived distance contraints, 90 dihedral angle restraints, 24 distance restraints from hydrogen bonds, and 19 H-C residual dipolar couplings
代表構造
選択基準: lowest energy
NMRアンサンブル
コンフォーマー選択の基準: structures with the lowest energy, no restraint violation, consistent with residual dipolar couplings 計算したコンフォーマーの数: 200 / 登録したコンフォーマーの数: 20