+Open data
-Basic information
Entry | Database: PDB / ID: 2qaz | ||||||
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Title | Structure of C. crescentus SspB ortholog | ||||||
Components | SspB Protein | ||||||
Keywords | Hydrolase activator / sspB / adaptor / ClpX / ssrA / UNKNOWN FUNCTION | ||||||
Function / homology | SspB-like / Stringent starvation protein B / SspB-like superfamily / Stringent starvation protein B / SH3 type barrels. / Roll / Mainly Beta / identical protein binding / Stringent starvation protein B Function and homology information | ||||||
Biological species | Caulobacter vibrioides (bacteria) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.7 Å | ||||||
Authors | Chien, P. / Grant, R.A. / Sauer, R.T. / Baker, T.A. | ||||||
Citation | Journal: To be Published Title: Structure of C. crescentus SspB ortholog Authors: Chien, P. / Grant, R.A. / Sauer, R.T. / Baker, T.A. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2qaz.cif.gz | 101.1 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2qaz.ent.gz | 79.2 KB | Display | PDB format |
PDBx/mmJSON format | 2qaz.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 2qaz_validation.pdf.gz | 457 KB | Display | wwPDB validaton report |
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Full document | 2qaz_full_validation.pdf.gz | 470.5 KB | Display | |
Data in XML | 2qaz_validation.xml.gz | 20.1 KB | Display | |
Data in CIF | 2qaz_validation.cif.gz | 27 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/qa/2qaz ftp://data.pdbj.org/pub/pdb/validation_reports/qa/2qaz | HTTPS FTP |
-Related structure data
Related structure data | |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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2 |
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Unit cell |
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Details | The asymmetric unit contains two copies of the dimeric biological assembly consisting of chain A with B and chain C with D |
-Components
#1: Protein | Mass: 14083.997 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Caulobacter vibrioides (bacteria) / Strain: CB15N / Gene: CC_2102 / Plasmid: pET28b / Species (production host): Escherichia coli / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21(DE3) / References: UniProt: Q9A6J2 #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 3.34 Å3/Da / Density % sol: 63.16 % |
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Crystal grow | Temperature: 300 K / Method: vapor diffusion, hanging drop / pH: 7.5 Details: 1.3 M (NH4)2SO4, 250 mM NaCl, 100mM Tris, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 300K |
-Data collection
Diffraction | Mean temperature: 110 K |
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Diffraction source | Source: SYNCHROTRON / Site: APS / Beamline: 24-ID-C / Wavelength: 1.07179 Å |
Detector | Type: ADSC QUANTUM 315 / Detector: CCD / Date: Aug 14, 2006 |
Radiation | Monochromator: crystal monochromator / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.07179 Å / Relative weight: 1 |
Reflection | Resolution: 2.7→50 Å / Num. all: 20967 / Num. obs: 20900 / % possible obs: 99.8 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 7.2 % / Rmerge(I) obs: 0.091 / Χ2: 1.041 / Net I/σ(I): 10 |
Reflection shell | Resolution: 2.7→2.8 Å / Redundancy: 5.5 % / Rmerge(I) obs: 0.351 / Num. unique all: 2071 / Χ2: 0.405 / % possible all: 99.9 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT / Resolution: 2.7→41.49 Å / Rfactor Rfree error: 0.008 / Data cutoff high absF: 2429220.25 / Data cutoff low absF: 0 / Isotropic thermal model: RESTRAINED / Cross valid method: THROUGHOUT / σ(F): 0 / Stereochemistry target values: Engh & Huber
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Solvent computation | Solvent model: FLAT MODEL / Bsol: 52.3 Å2 / ksol: 0.375 e/Å3 | ||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 56.6 Å2
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 2.7→41.49 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.7→2.87 Å / Rfactor Rfree error: 0.023 / Total num. of bins used: 6
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Xplor file |
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