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Open data
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Basic information
Entry | Database: PDB / ID: 2qas | ||||||
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Title | Crystal structure of Caulobacter crescentus SspB ortholog | ||||||
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![]() | Hydrolase activator / sspB / adaptor / ClpX / ssrA / UNKNOWN FUNCTION | ||||||
Function / homology | SspB-like / Stringent starvation protein B / SspB-like superfamily / Stringent starvation protein B / SH3 type barrels. / Roll / Mainly Beta / identical protein binding / Stringent starvation protein B![]() | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Chien, P. / Grant, R.A. / Sauer, R.T. / Baker, T.A. | ||||||
![]() | ![]() Title: Crystal structure of Caulobacter crescentus SspB ortholog Authors: Chien, P. / Grant, R.A. / Sauer, R.T. / Baker, T.A. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 38.5 KB | Display | ![]() |
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PDB format | ![]() | 26 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 422.3 KB | Display | ![]() |
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Full document | ![]() | 423.7 KB | Display | |
Data in XML | ![]() | 7.3 KB | Display | |
Data in CIF | ![]() | 8.8 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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Unit cell |
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Details | Biological assembly is a dimer formed from the monomer in the asymmetric unit by the operations: -x,-y,z + [1,1,0] |
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Components
#1: Protein | Mass: 17144.199 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
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#2: Protein/peptide | Mass: 2107.285 Da / Num. of mol.: 1 / Source method: obtained synthetically Details: synthetic ssrA pepitde with N-terminal solubilization tag |
#3: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.07 Å3/Da / Density % sol: 59.99 % |
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Crystal grow | Temperature: 300 K / Method: vapor diffusion, hanging drop / pH: 8 Details: 1.4 M (NH4)2SO4, 0.1 M Tris, pH 8.0, hanging drop, temperature 300K, VAPOR DIFFUSION, HANGING DROP |
-Data collection
Diffraction | Mean temperature: 110 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 315 / Detector: CCD / Date: Nov 2, 2006 |
Radiation | Monochromator: crystal monochromator / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97908 Å / Relative weight: 1 |
Reflection | Resolution: 2.55→50 Å / Num. all: 8146 / Num. obs: 7817 / % possible obs: 98 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 10.3 % / Rmerge(I) obs: 0.074 / Χ2: 1.161 / Net I/σ(I): 12.8 |
Reflection shell | Resolution: 2.55→2.64 Å / Redundancy: 5.8 % / Rmerge(I) obs: 0.377 / Num. unique all: 653 / Χ2: 0.384 / % possible all: 85.2 |
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Processing
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Refinement | Method to determine structure: ![]()
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Solvent computation | Solvent model: FLAT MODEL / Bsol: 44.361 Å2 / ksol: 0.332 e/Å3 | ||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 69.4 Å2
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 2.55→26.76 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.55→2.71 Å / Rfactor Rfree error: 0.037 / Total num. of bins used: 6
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Xplor file |
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