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- PDB-2psz: Structure of the PTP-like Phytase expressed by Selenomonas rumina... -
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Open data
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Basic information
Entry | Database: PDB / ID: 2psz | ||||||
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Title | Structure of the PTP-like Phytase expressed by Selenomonas ruminantium at low ionic strength | ||||||
![]() | Myo-inositol hexaphosphate phosphohydrolase | ||||||
![]() | HYDROLASE / PTP / protein tyrosine phosphatase / phytase / P-loop | ||||||
Function / homology | ![]() | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Gruninger, R.J. / Selinger, L.B. / Mosimann, S.C. | ||||||
![]() | ![]() Title: Effect of ionic strength and oxidation on the P-loop conformation of the protein tyrosine phosphatase-like phytase, PhyAsr. Authors: Gruninger, R.J. / Brent Selinger, L. / Mosimann, S.C. | ||||||
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 151.1 KB | Display | ![]() |
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PDB format | ![]() | 117.2 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 445.8 KB | Display | ![]() |
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Full document | ![]() | 448.3 KB | Display | |
Data in XML | ![]() | 29.2 KB | Display | |
Data in CIF | ![]() | 44.5 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 2pt0C ![]() 3d1hC ![]() 3d1oC ![]() 3d1qC ![]() 2b4pS S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 38987.910 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #2: Chemical | ChemComp-GOL / #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.17 Å3/Da / Density % sol: 61.14 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop / pH: 4.8 Details: 10% P8K, 200 mM NaCl, 50 mM Na-acetate (pH 4.8), VAPOR DIFFUSION, SITTING DROP, temperature 293K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 210 / Detector: CCD / Date: Oct 20, 2006 |
Radiation | Monochromator: double crystal / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.11 Å / Relative weight: 1 |
Reflection | Resolution: 2→50 Å / Num. all: 61360 / Num. obs: 61360 / % possible obs: 93.8 % / Observed criterion σ(F): 2 / Observed criterion σ(I): 1.5 / Redundancy: 3.5 % / Biso Wilson estimate: 19.2 Å2 / Rmerge(I) obs: 0.069 / Rsym value: 0.074 / Net I/σ(I): 15.6 |
Reflection shell | Resolution: 2→2.07 Å / Redundancy: 2 % / Rmerge(I) obs: 0.228 / Mean I/σ(I) obs: 2.97 / Num. unique all: 4135 / Rsym value: 0.224 / % possible all: 63.2 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ID 2B4P Resolution: 2→50 Å / σ(F): 1.5 / σ(I): 1.5 / Stereochemistry target values: Engh & Huber
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Refinement step | Cycle: LAST / Resolution: 2→50 Å
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