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Yorodumi- PDB-2pcf: THE COMPLEX OF CYTOCHROME F AND PLASTOCYANIN DETERMINED WITH PARA... -
+Open data
-Basic information
Entry | Database: PDB / ID: 2pcf | ||||||
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Title | THE COMPLEX OF CYTOCHROME F AND PLASTOCYANIN DETERMINED WITH PARAMAGNETIC NMR. BASED ON THE STRUCTURES OF CYTOCHROME F AND PLASTOCYANIN, 10 STRUCTURES | ||||||
Components |
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Keywords | COMPLEX (ELECTRON TRANSPORT PROTEINS) / ELECTRON TRANSPORT / PARAMAGNETIC / CHEMICAL SHIFT / COMPLEX FORMATION / DYNAMIC COMPLEX / PHOTOSYNTHESIS / PSEUDOCONTACT SHIFT | ||||||
Function / homology | Function and homology information chloroplast thylakoid membrane / photosynthesis / electron transfer activity / iron ion binding / copper ion binding / heme binding Similarity search - Function | ||||||
Biological species | Spinacia oleracea (spinach) Brassica rapa (field mustard) | ||||||
Method | SOLUTION NMR / DYNAMICS | ||||||
Authors | Ubbink, M. / Ejdeback, M. / Karlsson, B.G. / Bendall, D.S. | ||||||
Citation | Journal: Structure / Year: 1998 Title: The structure of the complex of plastocyanin and cytochrome f, determined by paramagnetic NMR and restrained rigid-body molecular dynamics. Authors: Ubbink, M. / Ejdeback, M. / Karlsson, B.G. / Bendall, D.S. #1: Journal: Protein Expr.Purif. / Year: 1997 Title: Effects of Codon Usage and Vector-Host Combinations on the Expression of Spinach Plastocyanin in Escherichia Coli Authors: Ejdeback, M. / Young, S. / Samuelsson, A. / Karlsson, B.G. #2: Journal: Structure / Year: 1994 Title: Crystal Structure of Chloroplast Cytochrome F Reveals a Novel Cytochrome Fold and Unexpected Heme Ligation Authors: Martinez, S.E. / Huang, D. / Szczepaniak, A. / Cramer, W.A. / Smith, J.L. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2pcf.cif.gz | 1 MB | Display | PDBx/mmCIF format |
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PDB format | pdb2pcf.ent.gz | 920.6 KB | Display | PDB format |
PDBx/mmJSON format | 2pcf.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/pc/2pcf ftp://data.pdbj.org/pub/pdb/validation_reports/pc/2pcf | HTTPS FTP |
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-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 10420.557 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Spinacia oleracea (spinach) / Cellular location: THYLAKOID LUMENThylakoid / Gene: PETE / Organelle: CHLOROPLAST / Plasmid: PSY2 / Species (production host): Escherichia coli / Cellular location (production host): PERIPLASM / Gene (production host): PETE / Production host: Escherichia coli BL21 (bacteria) / Strain (production host): BL21 / References: UniProt: P00289 |
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#2: Protein | Mass: 27318.189 Da / Num. of mol.: 1 / Fragment: SOLUBLE DOMAIN Source method: isolated from a genetically manipulated source Source: (gene. exp.) Brassica rapa (field mustard) / Cellular location: THYLAKOID MEMBRANEThylakoid / Gene: PETE / Organelle: CHLOROPLAST / Plasmid: PSY2 / Species (production host): Escherichia coli / Gene (production host): PETE / Production host: Escherichia coli BL21 (bacteria) / Strain (production host): BL21 / References: UniProt: P36438 |
#3: Chemical | ChemComp-CU / |
#4: Chemical | ChemComp-HEC / |
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR |
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NMR experiment | Type: 15N-HSQC |
-Sample preparation
Sample conditions | pH: 6.0 / Temperature: 300 K |
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Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
NMR spectrometer |
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-Processing
Software |
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NMR software |
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Refinement | Method: DYNAMICS / Software ordinal: 1 Details: REFINEMENT DETAILS CAN BE FOUND BELOW AND IN THE JRNL CITATION ABOVE. | ||||||||||||
NMR ensemble | Conformer selection criteria: REFER TO PUBLICATION / Conformers calculated total number: 9999 / Conformers submitted total number: 10 |