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Yorodumi- PDB-2p5g: Crystal structure of RB69 gp43 in complex with DNA with dAMP oppo... -
+Open data
-Basic information
Entry | Database: PDB / ID: 2p5g | ||||||
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Title | Crystal structure of RB69 gp43 in complex with DNA with dAMP opposite an abasic site analog in a 21mer template | ||||||
Components |
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Keywords | transferase/DNA / DNA POLYMERASE / ABASIC SITE / DNA LESION / dAMP / transferase-DNA COMPLEX | ||||||
Function / homology | Function and homology information bidirectional double-stranded viral DNA replication / Hydrolases; Acting on ester bonds; Exodeoxyribonucleases producing 5'-phosphomonoesters / 3'-5' exonuclease activity / DNA replication / DNA-directed DNA polymerase / DNA-directed DNA polymerase activity / nucleotide binding / DNA binding / metal ion binding Similarity search - Function | ||||||
Biological species | Enterobacteria phage RB69 (virus) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / Isomorphous Replacement / Resolution: 2.8 Å | ||||||
Authors | Zahn, K.E. / Belrhali, H. / Wallace, S.S. / Doublie, S. | ||||||
Citation | Journal: Biochemistry / Year: 2007 Title: Caught bending the a-rule: crystal structures of translesion DNA synthesis with a non-natural nucleotide. Authors: Zahn, K.E. / Belrhali, H. / Wallace, S.S. / Doublie, S. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2p5g.cif.gz | 735 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2p5g.ent.gz | 586.4 KB | Display | PDB format |
PDBx/mmJSON format | 2p5g.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/p5/2p5g ftp://data.pdbj.org/pub/pdb/validation_reports/p5/2p5g | HTTPS FTP |
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-Related structure data
Related structure data | 2oyqC 2ozmC 2ozsC 1rv2 C: citing same article (ref.) S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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2 |
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3 |
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4 |
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Unit cell |
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-Components
#1: DNA chain | Mass: 6300.051 Da / Num. of mol.: 4 / Source method: obtained synthetically #2: DNA chain | Mass: 4634.023 Da / Num. of mol.: 4 / Source method: obtained synthetically #3: Protein | Mass: 105827.570 Da / Num. of mol.: 4 / Mutation: D222A,D327A Source method: isolated from a genetically manipulated source Source: (gene. exp.) Enterobacteria phage RB69 (virus) / Genus: T4-like viruses / Gene: 43 / Species (production host): Escherichia coli / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21(DE3) / References: UniProt: Q38087, DNA-directed DNA polymerase #4: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.79 Å3/Da / Density % sol: 55.97 % | ||||||||||||||||||||||||||||||||
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 7 Details: PEG 2000 MME, sodium acetate, magnesium sulfate, beta-mercaptoethanol, HEPES 7.0, glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 293K | ||||||||||||||||||||||||||||||||
Components of the solutions |
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: APS / Beamline: 23-ID-D / Wavelength: 0.97857 Å |
Detector | Type: MARMOSAIC 300 mm CCD / Detector: CCD / Date: Aug 3, 2006 / Details: mirrors |
Radiation | Monochromator: DCM - pair of flat Si crystals / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97857 Å / Relative weight: 1 |
Reflection | Resolution: 2.8→50 Å / Num. all: 125168 / Num. obs: 125168 / % possible obs: 98.5 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 3.4 % / Rmerge(I) obs: 0.085 / Net I/σ(I): 14.3 |
Reflection shell | Resolution: 2.8→2.9 Å / Redundancy: 3.2 % / Rmerge(I) obs: 0.402 / Mean I/σ(I) obs: 2.6 / % possible all: 87.7 |
-Processing
Software |
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Refinement | Method to determine structure: Isomorphous Replacement Starting model: 1RV2 1rv2 Resolution: 2.8→30 Å / Cor.coef. Fo:Fc: 0.918 / Cor.coef. Fo:Fc free: 0.872 / SU B: 16.636 / SU ML: 0.334 / Cross valid method: THROUGHOUT / ESU R Free: 0.478 / Stereochemistry target values: MAXIMUM LIKELIHOOD
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: BABINET MODEL WITH MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 73.922 Å2
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Refinement step | Cycle: LAST / Resolution: 2.8→30 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.8→2.872 Å / Total num. of bins used: 20
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