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- PDB-2oqs: Structure of the hDLG/SAP97 PDZ2 in complex with HPV-18 papilloma... -
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Open data
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Basic information
Entry | Database: PDB / ID: 2oqs | ||||||
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Title | Structure of the hDLG/SAP97 PDZ2 in complex with HPV-18 papillomavirus E6 peptide | ||||||
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![]() | PEPTIDE BINDING PROTEIN / HPV E6 / hDLG PDZ domain / protein-peptide complex / PEPTIDE-BINDING PROTEIN | ||||||
Function / homology | ![]() regulation of voltage-gated potassium channel activity involved in ventricular cardiac muscle cell action potential repolarization / regulation of protein localization to synapse / regulation of potassium ion import / L27 domain binding / regulation of potassium ion export across plasma membrane / MPP7-DLG1-LIN7 complex / membrane raft organization / hard palate development / establishment of centrosome localization / negative regulation of p38MAPK cascade ...regulation of voltage-gated potassium channel activity involved in ventricular cardiac muscle cell action potential repolarization / regulation of protein localization to synapse / regulation of potassium ion import / L27 domain binding / regulation of potassium ion export across plasma membrane / MPP7-DLG1-LIN7 complex / membrane raft organization / hard palate development / establishment of centrosome localization / negative regulation of p38MAPK cascade / guanylate kinase activity / cortical microtubule organization / regulation of sodium ion transmembrane transport / embryonic skeletal system morphogenesis / astral microtubule organization / structural constituent of postsynaptic density / NrCAM interactions / lateral loop / reproductive structure development / immunological synapse formation / myelin sheath abaxonal region / peristalsis / cell projection membrane / Synaptic adhesion-like molecules / smooth muscle tissue development / bicellular tight junction assembly / positive regulation of potassium ion transport / node of Ranvier / regulation of ventricular cardiac muscle cell action potential / protein-containing complex localization / Trafficking of AMPA receptors / Assembly and cell surface presentation of NMDA receptors / establishment or maintenance of epithelial cell apical/basal polarity / amyloid precursor protein metabolic process / neurotransmitter receptor localization to postsynaptic specialization membrane / endothelial cell proliferation / lens development in camera-type eye / Activation of Ca-permeable Kainate Receptor / regulation of myelination / cortical actin cytoskeleton organization / branching involved in ureteric bud morphogenesis / establishment or maintenance of cell polarity / negative regulation of G1/S transition of mitotic cell cycle / positive regulation of actin filament polymerization / Negative regulation of NMDA receptor-mediated neuronal transmission / Unblocking of NMDA receptors, glutamate binding and activation / receptor clustering / phosphoprotein phosphatase activity / Long-term potentiation / immunological synapse / intercalated disc / basement membrane / lateral plasma membrane / potassium channel regulator activity / bicellular tight junction / phosphatase binding / negative regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction / T cell proliferation / negative regulation of T cell proliferation / cytoskeletal protein binding / Ras activation upon Ca2+ influx through NMDA receptor / actin filament polymerization / phosphatidylinositol 3-kinase/protein kinase B signal transduction / synaptic membrane / protein localization to plasma membrane / regulation of membrane potential / positive regulation of protein localization to plasma membrane / actin filament organization / adherens junction / postsynaptic density membrane / neuromuscular junction / cytoplasmic side of plasma membrane / negative regulation of ERK1 and ERK2 cascade / sarcolemma / kinase binding / cell-cell adhesion / negative regulation of epithelial cell proliferation / cell-cell junction / cell junction / regulation of cell shape / RAF/MAP kinase cascade / chemical synaptic transmission / basolateral plasma membrane / microtubule / transmembrane transporter binding / molecular adaptor activity / cadherin binding / neuron projection / membrane raft / apical plasma membrane / glutamatergic synapse / positive regulation of cell population proliferation / endoplasmic reticulum membrane / perinuclear region of cytoplasm / Golgi apparatus / negative regulation of transcription by RNA polymerase II / endoplasmic reticulum / extracellular exosome / nucleus / plasma membrane Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
![]() | Liu, Y. / Baleja, J.D. / Henry, G.D. / Hegde, R.S. | ||||||
![]() | ![]() Title: Solution structure of the hDlg/SAP97 PDZ2 domain and its mechanism of interaction with HPV-18 papillomavirus E6 protein. Authors: Liu, Y. / Henry, G.D. / Hegde, R.S. / Baleja, J.D. | ||||||
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 963.3 KB | Display | ![]() |
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PDB format | ![]() | 825.1 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
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-Validation report
Summary document | ![]() | 356.7 KB | Display | ![]() |
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Full document | ![]() | 733.1 KB | Display | |
Data in XML | ![]() | 54.2 KB | Display | |
Data in CIF | ![]() | 88.2 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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NMR ensembles |
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Components
#1: Protein | Mass: 10400.886 Da / Num. of mol.: 1 / Fragment: second PDZ domain, residues 318-406 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
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#2: Protein/peptide | Mass: 789.880 Da / Num. of mol.: 1 / Source method: obtained synthetically Details: This sequence occurs naturally in Homo sapiens (human) |
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||||||
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NMR experiment |
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NMR details | Text: The structure was determined using triple-resonance NMR spectroscopy. |
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Sample preparation
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Sample conditions |
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-NMR measurement
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M |
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Radiation wavelength | Relative weight: 1 |
NMR spectrometer | Type: Bruker AVANCE DRX / Manufacturer: Bruker / Model: AVANCE DRX / Field strength: 600 MHz |
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Processing
NMR software |
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Refinement | Method: simulated annealing / Software ordinal: 1 Details: the structures are based on a total of 1426 restraints, 1126 are NOE-derived distance constraints, 163 dihedral angle restraints, 50 distance restraints from hydrogen bonds and 87 NH residual dipolar couplings. | ||||||||||||||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||||||||||||||
NMR ensemble | Conformer selection criteria: all calculated structures submitted Conformers calculated total number: 30 / Conformers submitted total number: 30 |