+Open data
-Basic information
Entry | Database: PDB / ID: 2oam | ||||||
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Title | Apo RebH from Lechevalieria aerocolonigenes | ||||||
Components | Tryptophan halogenase | ||||||
Keywords | BIOSYNTHETIC PROTEIN/FLAVOPROTEIN / tryptophan-7-halogenase / flavin-binding / rebeccamycin biosynthesis / BIOSYNTHETIC PROTEIN-FLAVOPROTEIN COMPLEX | ||||||
Function / homology | Function and homology information | ||||||
Biological species | Lechevalieria aerocolonigenes (bacteria) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / FOURIER SYNTHESIS / Resolution: 2.3 Å | ||||||
Authors | Blasiak, L.C. / Drennan, C.L. | ||||||
Citation | Journal: Biochemistry / Year: 2007 Title: Chlorination by a long-lived intermediate in the mechanism of flavin-dependent halogenases(,). Authors: Yeh, E. / Blasiak, L.C. / Koglin, A. / Drennan, C.L. / Walsh, C.T. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2oam.cif.gz | 228.3 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2oam.ent.gz | 182.2 KB | Display | PDB format |
PDBx/mmJSON format | 2oam.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/oa/2oam ftp://data.pdbj.org/pub/pdb/validation_reports/oa/2oam | HTTPS FTP |
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-Related structure data
Related structure data | 2e4gSC 2oalC S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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Components on special symmetry positions |
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-Components
#1: Protein | Mass: 62540.070 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Lechevalieria aerocolonigenes (bacteria) Gene: rbmJ, rebH / Plasmid: pET28a / Species (production host): Escherichia coli / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21(DE3) / References: UniProt: Q8KHZ8 #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 3.51 Å3/Da / Density % sol: 64.93 % |
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Crystal grow | Temperature: 277 K / Method: hanging drop / pH: 7 Details: 1.2 M Na/K phosphate, pH 7.0, hanging drop, temperature 277K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: SSRL / Beamline: BL9-1 / Wavelength: 0.97946 Å |
Detector | Type: ADSC QUANTUM 315 / Detector: CCD / Date: Jul 7, 2006 / Details: Inter-frame total dead time: 3s. |
Radiation | Monochromator: Side-scattering cuberoot I-beam bent single crystal Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97946 Å / Relative weight: 1 |
Reflection | Resolution: 2.3→41.7 Å / Num. all: 75915 / Num. obs: 75915 / % possible obs: 99.7 % / Redundancy: 4.5 % / Biso Wilson estimate: 49.8 Å2 / Rsym value: 0.079 / Net I/σ(I): 18.2 |
Reflection shell | Resolution: 2.3→2.38 Å / Redundancy: 4.5 % / Mean I/σ(I) obs: 2.4 / Num. unique all: 7545 / Rsym value: 0.495 / % possible all: 99.6 |
-Processing
Software |
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Refinement | Method to determine structure: FOURIER SYNTHESIS Starting model: PDB ENTRY 2E4G Resolution: 2.3→41.7 Å / Isotropic thermal model: isotropic / Cross valid method: THROUGHOUT / σ(F): 0 / Stereochemistry target values: Engh & Huber
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Solvent computation | Bsol: 40.41 Å2 | ||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 38.989 Å2
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 2.3→41.7 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.3→2.38 Å
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Xplor file |
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