+Open data
-Basic information
Entry | Database: PDB / ID: 2nb1 | ||||||
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Title | P63/p73 hetero-tetramerisation domain | ||||||
Components |
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Keywords | TRANSCRIPTION / p63 / p73 / tetramerization domain / hetero tetramer / transcription factor | ||||||
Function / homology | Function and homology information ectoderm and mesoderm interaction / epidermal cell division / cloacal septation / positive regulation of somatic stem cell population maintenance / positive regulation of lung ciliated cell differentiation / prostatic bud formation / negative regulation of mesoderm development / female genitalia morphogenesis / establishment of planar polarity / positive regulation of keratinocyte proliferation ...ectoderm and mesoderm interaction / epidermal cell division / cloacal septation / positive regulation of somatic stem cell population maintenance / positive regulation of lung ciliated cell differentiation / prostatic bud formation / negative regulation of mesoderm development / female genitalia morphogenesis / establishment of planar polarity / positive regulation of keratinocyte proliferation / negative regulation of keratinocyte differentiation / squamous basal epithelial stem cell differentiation involved in prostate gland acinus development / polarized epithelial cell differentiation / negative regulation of intracellular estrogen receptor signaling pathway / proximal/distal pattern formation / negative regulation of cardiac muscle cell proliferation / positive regulation of fibroblast apoptotic process / positive regulation of cell cycle G1/S phase transition / WW domain binding / skin morphogenesis / cranial skeletal system development / sympathetic nervous system development / post-anal tail morphogenesis / embryonic forelimb morphogenesis / embryonic hindlimb morphogenesis / TP53 Regulates Transcription of Death Receptors and Ligands / Activation of PUMA and translocation to mitochondria / Regulation of TP53 Activity through Association with Co-factors / hair follicle morphogenesis / positive regulation of oligodendrocyte differentiation / positive regulation of Notch signaling pathway / regulation of epidermal cell division / TP53 regulates transcription of several additional cell death genes whose specific roles in p53-dependent apoptosis remain uncertain / positive regulation of stem cell proliferation / TP53 Regulates Transcription of Caspase Activators and Caspases / epithelial cell development / odontogenesis of dentin-containing tooth / negative regulation of cellular senescence / TP53 Regulates Transcription of Genes Involved in Cytochrome C Release / keratinocyte proliferation / negative regulation of neuron differentiation / intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator / establishment of skin barrier / mismatch repair / Pyroptosis / positive regulation of osteoblast differentiation / keratinocyte differentiation / MDM2/MDM4 family protein binding / Notch signaling pathway / response to organonitrogen compound / regulation of mitotic cell cycle / transcription corepressor binding / kidney development / skeletal system development / stem cell proliferation / positive regulation of apoptotic signaling pathway / determination of adult lifespan / promoter-specific chromatin binding / TP53 Regulates Metabolic Genes / protein tetramerization / RNA polymerase II transcription regulatory region sequence-specific DNA binding / intrinsic apoptotic signaling pathway in response to DNA damage / cellular senescence / p53 binding / cell junction / RUNX1 regulates transcription of genes involved in differentiation of HSCs / spermatogenesis / regulation of gene expression / DNA-binding transcription activator activity, RNA polymerase II-specific / neuron apoptotic process / DNA-binding transcription factor binding / RNA polymerase II-specific DNA-binding transcription factor binding / transcription by RNA polymerase II / positive regulation of MAPK cascade / damaged DNA binding / transcription cis-regulatory region binding / DNA-binding transcription factor activity, RNA polymerase II-specific / regulation of cell cycle / chromatin remodeling / response to xenobiotic stimulus / positive regulation of apoptotic process / cell cycle / RNA polymerase II cis-regulatory region sequence-specific DNA binding / DNA-binding transcription factor activity / negative regulation of cell population proliferation / intracellular membrane-bounded organelle / negative regulation of DNA-templated transcription / apoptotic process / DNA damage response / chromatin binding / dendrite / chromatin / regulation of transcription by RNA polymerase II / protein kinase binding / positive regulation of DNA-templated transcription / Golgi apparatus / negative regulation of transcription by RNA polymerase II / positive regulation of transcription by RNA polymerase II / DNA binding / nucleoplasm Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | SOLUTION NMR / molecular dynamics | ||||||
Model details | closest to the average, model1 | ||||||
Authors | Gebel, J. / Buchner, L. / Loehr, F.M. / Luh, L.M. / Coutandin, D. / Guentert, P. / Doetsch, V. | ||||||
Citation | Journal: Cell Death Differ. / Year: 2016 Title: Mechanism of TAp73 inhibition by Delta Np63 and structural basis of p63/p73 hetero-tetramerization. Authors: Gebel, J. / Luh, L.M. / Coutandin, D. / Osterburg, C. / Lohr, F. / Schafer, B. / Frombach, A.S. / Sumyk, M. / Buchner, L. / Krojer, T. / Salah, E. / Mathea, S. / Guntert, P. / Knapp, S. / Dotsch, V. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2nb1.cif.gz | 1.4 MB | Display | PDBx/mmCIF format |
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PDB format | pdb2nb1.ent.gz | 1.2 MB | Display | PDB format |
PDBx/mmJSON format | 2nb1.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/nb/2nb1 ftp://data.pdbj.org/pub/pdb/validation_reports/nb/2nb1 | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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NMR ensembles |
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-Components
#1: Protein | Mass: 7323.190 Da / Num. of mol.: 2 Fragment: Tetramerization domain of 63, UNP residues 397-455 Mutation: K21E Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: KET, P63, P73H, P73L, TP63, TP73L / Production host: Escherichia coli (E. coli) / Strain (production host): BL21 / References: UniProt: Q9H3D4 #2: Protein/peptide | Mass: 6034.894 Da / Num. of mol.: 2 Fragment: Tetramerization domain of p73, UNP residues 351-398 Mutation: E15K Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: P73, TP73 / Production host: Escherichia coli (E. coli) / Strain (production host): BL21 / References: UniProt: O15350 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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NMR experiment |
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-Sample preparation
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Sample conditions |
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-NMR measurement
NMR spectrometer |
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-Processing
NMR software |
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Refinement | Method: molecular dynamics / Software ordinal: 1 | ||||||||||||||||||||||||||||||||
NMR constraints | NOE constraints total: 2582 / NOE intraresidue total count: 412 / NOE long range total count: 850 / NOE medium range total count: 614 / NOE sequential total count: 706 / Hydrogen bond constraints total count: 440 / Protein chi angle constraints total count: 0 / Protein other angle constraints total count: 0 / Protein phi angle constraints total count: 180 / Protein psi angle constraints total count: 180 | ||||||||||||||||||||||||||||||||
NMR representative | Selection criteria: closest to the average | ||||||||||||||||||||||||||||||||
NMR ensemble | Average torsion angle constraint violation: 4.96 ° / Conformer selection criteria: target function / Conformers calculated total number: 200 / Conformers submitted total number: 20 / Maximum torsion angle constraint violation: 6.66 ° / Maximum upper distance constraint violation: 0.17 Å | ||||||||||||||||||||||||||||||||
NMR ensemble rms | Distance rms dev: 0.0076 Å |