+Open data
-Basic information
Entry | Database: PDB / ID: 2n7p | ||||||
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Title | Solution structure of PDZ domain | ||||||
Components | Putative uncharacterized protein | ||||||
Keywords | TRANSFERASE / PDZ | ||||||
Function / homology | Function and homology information | ||||||
Biological species | Trypanosoma brucei brucei TREU927 (eukaryote) | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
Model details | lowest energy, model1 | ||||||
Authors | Mei, S. | ||||||
Citation | Journal: To be Published Title: Solution structure of Q388A3 PDZ domain from Trypanosoma brucei and its interaction with OMP-like peptide Authors: Mei, S. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2n7p.cif.gz | 694.8 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2n7p.ent.gz | 592.6 KB | Display | PDB format |
PDBx/mmJSON format | 2n7p.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 2n7p_validation.pdf.gz | 532.5 KB | Display | wwPDB validaton report |
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Full document | 2n7p_full_validation.pdf.gz | 733.7 KB | Display | |
Data in XML | 2n7p_validation.xml.gz | 49 KB | Display | |
Data in CIF | 2n7p_validation.cif.gz | 63.9 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/n7/2n7p ftp://data.pdbj.org/pub/pdb/validation_reports/n7/2n7p | HTTPS FTP |
-Related structure data
Similar structure data | |
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Other databases |
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 10985.445 Da / Num. of mol.: 1 / Fragment: UNP RESIDUES 1528-1630 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Trypanosoma brucei brucei TREU927 (eukaryote) Strain: 927/4 GUTat10.1 / Gene: Tb10.61.1430 / Production host: Escherichia coli (E. coli) / References: UniProt: Q388A3 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR |
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NMR experiment | Type: 2D 1H-15N HSQC |
-Sample preparation
Details | Contents: 100 mM sodium chloride-1, 90% H2O/10% D2O / Solvent system: 90% H2O/10% D2O |
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Sample | Conc.: 100 mM / Component: sodium chloride-1 |
Sample conditions | Ionic strength: 0.1 / pH: 6.5 / Pressure: ambient / Temperature: 20 K |
-NMR measurement
NMR spectrometer | Type: Bruker DMX / Manufacturer: Bruker / Model: DMX / Field strength: 600 MHz |
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-Processing
NMR software |
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Refinement | Method: simulated annealing / Software ordinal: 1 | |||||||||
NMR representative | Selection criteria: lowest energy | |||||||||
NMR ensemble | Conformer selection criteria: target function / Conformers calculated total number: 500 / Conformers submitted total number: 20 / Representative conformer: 1 |