cellular response to peptide / phosphatidylinositol-5-phosphate binding / nucleotide-excision repair factor 3 complex / phosphatidylinositol-3-phosphate binding / transcription factor TFIIH core complex / transcription factor TFIIH holo complex / RNA Pol II CTD phosphorylation and interaction with CE / Formation of the Early Elongation Complex / mRNA Capping / TP53 Regulates Transcription of DNA Repair Genes ...cellular response to peptide / phosphatidylinositol-5-phosphate binding / nucleotide-excision repair factor 3 complex / phosphatidylinositol-3-phosphate binding / transcription factor TFIIH core complex / transcription factor TFIIH holo complex / RNA Pol II CTD phosphorylation and interaction with CE / Formation of the Early Elongation Complex / mRNA Capping / TP53 Regulates Transcription of DNA Repair Genes / RNA Polymerase II Promoter Escape / RNA Polymerase II Transcription Pre-Initiation And Promoter Opening / RNA Polymerase II Transcription Initiation / RNA Polymerase II Transcription Initiation And Promoter Clearance / RNA Polymerase II Pre-transcription Events / Formation of TC-NER Pre-Incision Complex / RNA Polymerase I Promoter Escape / Gap-filling DNA repair synthesis and ligation in TC-NER / transcription by RNA polymerase I / Dual incision in TC-NER / maternal process involved in female pregnancy / erythrocyte differentiation / ubiquitin binding / nucleotide-excision repair / transcription initiation at RNA polymerase II promoter / protein destabilization / ubiquitin-dependent protein catabolic process / transcription by RNA polymerase II / transcription cis-regulatory region binding / DNA-binding transcription factor activity, RNA polymerase II-specific / RNA polymerase II cis-regulatory region sequence-specific DNA binding / DNA-binding transcription factor activity / DNA repair / regulation of DNA-templated transcription / chromatin / regulation of transcription by RNA polymerase II / positive regulation of DNA-templated transcription / nucleoplasm / nucleus / metal ion binding / cytosol Similarity search - Function
Mass: 2359.483 Da / Num. of mol.: 1 / Fragment: Transactivation Domain 1, UNP residues 22-40 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: KLF1, EKLF / Production host: Escherichia coli (E. coli) / References: UniProt: Q13351
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Experimental details
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Experiment
Experiment
Method: SOLUTION NMR
NMR experiment
Conditions-ID
Experiment-ID
Solution-ID
Type
1
1
1
2D 1H-15N HSQC
1
2
1
3D (H)CCH-TOCSY
1
3
1
3DCBCA(CO)NH
1
4
1
3D HNCO
1
5
1
3D HN(CA)CB
1
6
1
3D 1H-15N NOESY
1
7
2
2D 1H-13C HSQC
1
8
2
3D 1H-13C NOESY
1
9
2
3D intermolecular NOESY
1
10
3
2D 1H-15N HSQC
1
11
3
3D (H)CCH-TOCSY
1
12
3
3DCBCA(CO)NH
1
13
3
3D HN(CA)CB
1
14
3
3D 1H-15N NOESY
1
15
4
2D 1H-13C HSQC aliphatic
1
16
4
2D 1H-13C HSQC aromatic
1
17
4
3D 1H-13C NOESY aliphatic
1
18
4
3D 1H-13C NOESY aromatic
1
19
4
3D intermolecular NOESY
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Sample preparation
Details
Solution-ID
Contents
Solvent system
1
0.8 mM [U-100% 13C; U-100% 15N] RNA polymerase II transcription factor B subunit 1, 2 mM N-terminal transactivation domain of Krueppel-like factor 1, 90% H2O/10% D2O
90% H2O/10% D2O
2
0.8 mM [U-100% 13C; U-100% 15N] RNA polymerase II transcription factor B subunit 1, 2 mM N-terminal transactivation domain of Krueppel-like factor 1, 100% D2O
100% D2O
3
0.8 mM [U-100% 13C; U-100% 15N] N-terminal transactivation domain of Krueppel-like factor 1, 2 mM RNA polymerase II transcription factor B subunit 1, 90% H2O/10% D2O
90% H2O/10% D2O
4
0.8 mM [U-100% 13C; U-100% 15N] N-terminal transactivation domain of Krueppel-like factor 1, 2 mM RNA polymerase II transcription factor B subunit 1, 100% D2O
100% D2O
Sample
Conc. (mg/ml)
Component
Isotopic labeling
Solution-ID
0.8mM
RNA polymerase II transcription factor B subunit 1-1
[U-100% 13C; U-100% 15N]
1
2mM
N-terminal transactivation domain of Krueppel-like factor 1-2
1
0.8mM
RNA polymerase II transcription factor B subunit 1-3
[U-100% 13C; U-100% 15N]
2
2mM
N-terminal transactivation domain of Krueppel-like factor 1-4
2
0.8mM
N-terminal transactivation domain of Krueppel-like factor 1-5
[U-100% 13C; U-100% 15N]
3
2mM
RNA polymerase II transcription factor B subunit 1-6
3
0.8mM
N-terminal transactivation domain of Krueppel-like factor 1-7
[U-100% 13C; U-100% 15N]
4
2mM
RNA polymerase II transcription factor B subunit 1-8
NOE constraints total: 2737 / NOE intraresidue total count: 1453 / NOE long range total count: 540 / NOE medium range total count: 2015 / NOE sequential total count: 486 / Protein phi angle constraints total count: 119 / Protein psi angle constraints total count: 119
NMR representative
Selection criteria: lowest energy
NMR ensemble
Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 260 / Conformers submitted total number: 20
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