0.8 mM [U-100% 13C; U-100% 15N] RNA polymerase II transcription factor B subunit 1, 2 mM N-terminal transactivation domain of Krueppel-like factor 1, 90% H2O/10% D2O
90% H2O/10% D2O
2
0.8 mM [U-100% 13C; U-100% 15N] RNA polymerase II transcription factor B subunit 1, 2 mM N-terminal transactivation domain of Krueppel-like factor 1, 100% D2O
100% D2O
3
0.8 mM [U-100% 13C; U-100% 15N] N-terminal transactivation domain of Krueppel-like factor 1, 2 mM RNA polymerase II transcription factor B subunit 1, 90% H2O/10% D2O
90% H2O/10% D2O
4
0.8 mM [U-100% 13C; U-100% 15N] N-terminal transactivation domain of Krueppel-like factor 1, 2 mM RNA polymerase II transcription factor B subunit 1, 100% D2O
100% D2O
試料
濃度 (mg/ml)
構成要素
Isotopic labeling
Solution-ID
0.8mM
RNA polymerase II transcription factor B subunit 1-1
[U-100% 13C; U-100% 15N]
1
2mM
N-terminal transactivation domain of Krueppel-like factor 1-2
1
0.8mM
RNA polymerase II transcription factor B subunit 1-3
[U-100% 13C; U-100% 15N]
2
2mM
N-terminal transactivation domain of Krueppel-like factor 1-4
2
0.8mM
N-terminal transactivation domain of Krueppel-like factor 1-5
[U-100% 13C; U-100% 15N]
3
2mM
RNA polymerase II transcription factor B subunit 1-6
3
0.8mM
N-terminal transactivation domain of Krueppel-like factor 1-7
[U-100% 13C; U-100% 15N]
4
2mM
RNA polymerase II transcription factor B subunit 1-8
4
試料状態
イオン強度: 0 / pH: 6.5 / 圧: ambient / 温度: 300 K
-
NMR測定
NMRスペクトロメーター
タイプ
製造業者
モデル
磁場強度 (MHz)
Spectrometer-ID
Varian INOVA
Varian
INOVA
500
1
Varian INOVA
Varian
INOVA
600
2
Varian INOVA
Varian
INOVA
800
3
-
解析
NMR software
名称: CNS / 分類: 精密化
精密化
手法: torsion angle dynamics / ソフトェア番号: 1
NMR constraints
NOE constraints total: 2737 / NOE intraresidue total count: 1453 / NOE long range total count: 540 / NOE medium range total count: 2015 / NOE sequential total count: 486 / Protein phi angle constraints total count: 119 / Protein psi angle constraints total count: 119
代表構造
選択基準: lowest energy
NMRアンサンブル
コンフォーマー選択の基準: structures with the lowest energy 計算したコンフォーマーの数: 260 / 登録したコンフォーマーの数: 20