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- PDB-4g7x: Crystal structure of a complex between the CTXphi pIII N-terminal... -
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Open data
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Basic information
Entry | Database: PDB / ID: 4g7x | ||||||
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Title | Crystal structure of a complex between the CTXphi pIII N-terminal domain and the Vibrio cholerae TolA C-terminal domain | ||||||
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![]() | PROTEIN BINDING/PROTEIN BINDING / Membrane / PROTEIN BINDING-PROTEIN BINDING complex | ||||||
Function / homology | ![]() bacteriocin transport / toxin transmembrane transporter activity / membrane => GO:0016020 Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Kolappan, S. / Ford, C.G. / Craig, L. | ||||||
![]() | ![]() Title: Crystal Structures of a CTX{varphi} pIII Domain Unbound and in Complex with a Vibrio cholerae TolA Domain Reveal Novel Interaction Interfaces. Authors: Ford, C.G. / Kolappan, S. / Phan, H.T. / Waldor, M.K. / Winther-Larsen, H.C. / Craig, L. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 54.9 KB | Display | ![]() |
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PDB format | ![]() | 42.2 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 428.6 KB | Display | ![]() |
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Full document | ![]() | 430.5 KB | Display | |
Data in XML | ![]() | 11.5 KB | Display | |
Data in CIF | ![]() | 16.4 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 11509.833 Da / Num. of mol.: 1 / Fragment: N-TERMINAL DOMAIN Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
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#2: Protein | Mass: 15072.992 Da / Num. of mol.: 1 / Fragment: C-TERMINAL DOMAIN Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
#3: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 1.91 Å3/Da / Density % sol: 35.73 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 6 Details: 25 % PEG 6000, 100 mM MES, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MARMOSAIC 325 mm CCD / Detector: CCD / Date: Dec 13, 2011 / Details: mirrors |
Radiation | Monochromator: Double crystal monochromator, Si(111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 1.44→39.9 Å / Num. all: 37355 / Num. obs: 35426 / % possible obs: 99.6 % / Observed criterion σ(F): 2 / Observed criterion σ(I): 4.4 / Redundancy: 6.9 % / Biso Wilson estimate: 19.8 Å2 / Rsym value: 0.042 / Net I/σ(I): 27.3 |
Reflection shell | Resolution: 1.44→1.48 Å / Redundancy: 4.6 % / Mean I/σ(I) obs: 4.4 / Num. unique all: 257179 / Rsym value: 0.329 / % possible all: 95.5 |
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Processing
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Refinement | Method to determine structure: ![]()
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | |||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 14.936 Å2
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Refinement step | Cycle: LAST / Resolution: 1.44→39.9 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 1.44→1.482 Å / Total num. of bins used: 20
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