+Open data
-Basic information
Entry | Database: PDB / ID: 2my5 | ||||||
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Title | Solution Structure of KstB-PCP in kosinostatin biosynthesis | ||||||
Components | Peptidyl carrier protein | ||||||
Keywords | TRANSPORT PROTEIN / Ppant binnding domain | ||||||
Function / homology | Function and homology information ACP-like / Non-ribosomal Peptide Synthetase Peptidyl Carrier Protein; Chain A / Phosphopantetheine attachment site / ACP-like superfamily / Carrier protein (CP) domain profile. / Phosphopantetheine binding ACP domain / Orthogonal Bundle / Mainly Alpha Similarity search - Domain/homology | ||||||
Biological species | Micromonospora sp. TP-A0468 (bacteria) | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
Model details | fewest violations and lowest energy, model1 | ||||||
Authors | Zhao, B. / Lan, W. / Wang, C. / Tang, G. / Cao, C. | ||||||
Citation | Journal: To be Published Title: 1H and 15N Assigned Chemical Shifts for KstB-PCP Authors: Zhao, B. / Lan, W. / Wang, C. / Tang, G. / Cao, C. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2my5.cif.gz | 537.1 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2my5.ent.gz | 452.5 KB | Display | PDB format |
PDBx/mmJSON format | 2my5.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/my/2my5 ftp://data.pdbj.org/pub/pdb/validation_reports/my/2my5 | HTTPS FTP |
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-Related structure data
Related structure data | 2my6C 2my0 2my4 C: citing same article (ref.) |
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Similar structure data | |
Other databases |
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 9664.883 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Micromonospora sp. TP-A0468 (bacteria) / Gene: KstB2 / Production host: Escherichia coli (E. coli) / Strain (production host): Rosetta(DE3) / References: UniProt: A0A023GUP0 |
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#2: Chemical | ChemComp-PN7 / |
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR |
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NMR experiment | Type: 3D CBCA(CO)NH |
-Sample preparation
Details | Contents: 2 mM [U-99% 13C; U-99% 15N] KstB-PCP(holo)-1, 90% H2O, 10% D2O Solvent system: 90% H2O/10% D2O |
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Sample | Conc.: 2 mM / Component: KstB-PCP(holo)-1 / Isotopic labeling: [U-99% 13C; U-99% 15N] |
Sample conditions | pH: 7 / Temperature units: K |
-NMR measurement
NMR spectrometer | Type: AGILENT INOVA / Manufacturer: Agilent / Model: INOVA / Field strength: 600 MHz |
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-Processing
NMR software | Name: CNS / Classification: refinement |
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Refinement | Method: simulated annealing / Software ordinal: 1 |
NMR representative | Selection criteria: fewest violations and lowest energy |
NMR ensemble | Conformer selection criteria: 20 / Conformers calculated total number: 100 / Conformers submitted total number: 20 / Representative conformer: 1 |