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Yorodumi- PDB-2mx9: NMR structure of N-terminal domain from A. ventricosus minor ampu... -
+Open data
-Basic information
Entry | Database: PDB / ID: 2mx9 | ||||||
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Title | NMR structure of N-terminal domain from A. ventricosus minor ampullate spidroin (MiSp) at pH 5.5 | ||||||
Components | Minor ampullate spidroin | ||||||
Keywords | STRUCTURAL PROTEIN | ||||||
Function / homology | Function and homology information | ||||||
Biological species | Araneus ventricosus (diadem spider) | ||||||
Method | SOLUTION NMR / simulated annealing, torsion angle dynamics | ||||||
Model details | closest to the average, model1 | ||||||
Authors | Otikovs, M. / Jaudzems, K. / Chen, G. / Nordling, K. / Rising, A. / Johansson, J. | ||||||
Citation | Journal: Chembiochem / Year: 2015 Title: Diversified Structural Basis of a Conserved Molecular Mechanism for pH-Dependent Dimerization in Spider Silk N-Terminal Domains. Authors: Otikovs, M. / Chen, G. / Nordling, K. / Landreh, M. / Meng, Q. / Jornvall, H. / Kronqvist, N. / Rising, A. / Johansson, J. / Jaudzems, K. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2mx9.cif.gz | 1.4 MB | Display | PDBx/mmCIF format |
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PDB format | pdb2mx9.ent.gz | 1.3 MB | Display | PDB format |
PDBx/mmJSON format | 2mx9.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 2mx9_validation.pdf.gz | 414.9 KB | Display | wwPDB validaton report |
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Full document | 2mx9_full_validation.pdf.gz | 612.5 KB | Display | |
Data in XML | 2mx9_validation.xml.gz | 87.5 KB | Display | |
Data in CIF | 2mx9_validation.cif.gz | 129.6 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/mx/2mx9 ftp://data.pdbj.org/pub/pdb/validation_reports/mx/2mx9 | HTTPS FTP |
-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 14067.841 Da / Num. of mol.: 2 / Fragment: N-terminal domain residues 23-151 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Araneus ventricosus (diadem spider) / Production host: Escherichia coli (E. coli) / Strain (production host): BL21(DE3) / References: UniProt: K4MTL7 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Details |
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Sample |
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Sample conditions | Ionic strength: 0.04 / pH: 5.5 / Pressure: ambient / Temperature: 295 K |
-NMR measurement
NMR spectrometer |
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-Processing
NMR software |
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Refinement | Method: simulated annealing, torsion angle dynamics / Software ordinal: 1 | ||||||||||||||||||||||||||||||||||||||||
NMR constraints | NOE constraints total: 4091 | ||||||||||||||||||||||||||||||||||||||||
NMR representative | Selection criteria: closest to the average | ||||||||||||||||||||||||||||||||||||||||
NMR ensemble | Conformer selection criteria: target function / Conformers calculated total number: 100 / Conformers submitted total number: 20 |