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- PDB-2msp: MAJOR SPERM PROTEIN, BETA ISOFORM, ENGINEERED C59S/T90C MUTANT, P... -
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Open data
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Basic information
Entry | Database: PDB / ID: 2msp | ||||||
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Title | MAJOR SPERM PROTEIN, BETA ISOFORM, ENGINEERED C59S/T90C MUTANT, PUTATIVE SUBFILAMENT STRUCTURE, PH 8.5 | ||||||
![]() | MAJOR SPERM PROTEIN | ||||||
![]() | CELL MOTILITY PROTEIN / CYTOSKELETAL PROTEIN / NEMATODE SPERM CELL MOTILITY PROTEIN / FILAMENTOUS PROTEIN STRUCTURE | ||||||
Function / homology | ![]() | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Bullock, T.L. / Mccoy, A.J. / Stewart, M. | ||||||
![]() | ![]() Title: Structural basis for amoeboid motility in nematode sperm. Authors: Bullock, T.L. / McCoy, A.J. / Kent, H.M. / Roberts, T.M. / Stewart, M. #1: ![]() Title: 2.5 A Resolution Crystal Structure of the Motile Major Sperm Protein (Msp) of Ascaris Suum Authors: Bullock, T.L. / Roberts, T.M. / Stewart, M. #2: ![]() Title: Worm Sperm and Advances in Cell Locomotion Authors: Theriot, J.A. #3: ![]() Title: Crystallization of the Motile Major Sperm Protein (Msp) of the Nematode Ascaris Suum Authors: Stewart, M. / King, K.L. / Roberts, T.M. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 203 KB | Display | ![]() |
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PDB format | ![]() | 163.7 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 421.4 KB | Display | ![]() |
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Full document | ![]() | 509.8 KB | Display | |
Data in XML | ![]() | 31.2 KB | Display | |
Data in CIF | ![]() | 43.9 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 1mspS S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 14120.876 Da / Num. of mol.: 8 / Mutation: C59S, T90C Source method: isolated from a genetically manipulated source Details: BETA ISOFORM / Source: (gene. exp.) ![]() ![]() ![]() |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.25 Å3/Da / Density % sol: 62.21 % | ||||||||||||||||||||||||||||||||||||||||
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Crystal grow | pH: 8.5 Details: PROTEIN WAS CRYSTALLIZED FROM 16% PEG 6000, 80MM AMMONIUM SULFATE, 100MM TRIS - HCL, PH 8.5 | ||||||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 20 ℃ / pH: 7.4 / Method: vapor diffusion | ||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 293 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MARRESEARCH / Detector: IMAGE PLATE / Date: Jan 1, 1997 / Details: OPTICS |
Radiation | Monochromator: MONOCHROMATOR / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9 Å / Relative weight: 1 |
Reflection | Resolution: 3.3→50 Å / Num. obs: 23854 / % possible obs: 100 % / Observed criterion σ(I): 0 / Redundancy: 11.7 % / Rmerge(I) obs: 0.118 / Net I/σ(I): 5.7 |
Reflection | *PLUS Num. measured all: 279016 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 1MSP Resolution: 3.3→10 Å / Isotropic thermal model: TNT BCORREL / σ(F): 0 / Stereochemistry target values: TNT
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Solvent computation | Solvent model: DEFAULT METHOD OF TNT / Bsol: 294 Å2 / ksol: 0.05 e/Å3 | ||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 3.3→10 Å
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Refine LS restraints |
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Software | *PLUS Name: TNT / Version: 5D / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS Rfactor Rfree: 0.263 | ||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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