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- PDB-1msp: MAJOR SPERM PROTEIN, ALPHA ISOFORM (RECOMBINANT), PH 4.6 -

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Basic information

Entry
Database: PDB / ID: 1msp
TitleMAJOR SPERM PROTEIN, ALPHA ISOFORM (RECOMBINANT), PH 4.6
ComponentsMAJOR SPERM PROTEIN
KeywordsCELL MOTILITY PROTEIN / CYTOSKELETAL PROTEIN / SPERM
Function / homology
Function and homology information


pseudopodium / cytoskeleton / cytoplasm
Similarity search - Function
: / Major sperm protein (MSP) domain / MSP (Major sperm protein) domain / Major sperm protein (MSP) domain profile. / PapD-like superfamily / Immunoglobulin-like fold / Immunoglobulins / Immunoglobulin-like / Sandwich / Mainly Beta
Similarity search - Domain/homology
Major sperm protein isoform alpha
Similarity search - Component
Biological speciesAscaris suum (pig roundworm)
MethodX-RAY DIFFRACTION / Resolution: 2.5 Å
AuthorsBullock, T.L. / Roberts, T.M. / Stewart, M.
Citation
Journal: J.Mol.Biol. / Year: 1996
Title: 2.5 A resolution crystal structure of the motile major sperm protein (MSP) of Ascaris suum.
Authors: Bullock, T.L. / Roberts, T.M. / Stewart, M.
#1: Journal: Cell(Cambridge,Mass.) / Year: 1996
Title: Worm Sperm and Advances in Cell Locomotion
Authors: Theriot, J.A.
#2: Journal: J.Struct.Biol. / Year: 1996
Title: New Crystal Forms of the Motile Major Sperm Protein (Msp) of Ascaris Suum
Authors: Bullock, T.L. / Parthasarathy, G. / King, K.L. / Kent, H.M. / Roberts, T.M. / Stewart, M.
#3: Journal: Curr.Opin.Cell Biol. / Year: 1995
Title: Nematode Sperm Locomotion
Authors: Roberts, T.M. / Stewart, M.
History
DepositionMay 20, 1996Processing site: BNL
Revision 1.0Dec 7, 1996Provider: repository / Type: Initial release
Revision 1.1Mar 24, 2008Group: Version format compliance
Revision 1.2Jul 13, 2011Group: Version format compliance
Revision 1.3Feb 14, 2024Group: Data collection / Database references / Other
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_database_status
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession / _pdbx_database_status.process_site

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: MAJOR SPERM PROTEIN
B: MAJOR SPERM PROTEIN


Theoretical massNumber of molelcules
Total (without water)28,5562
Polymers28,5562
Non-polymers00
Water1,33374
1
A: MAJOR SPERM PROTEIN
B: MAJOR SPERM PROTEIN

A: MAJOR SPERM PROTEIN
B: MAJOR SPERM PROTEIN


Theoretical massNumber of molelcules
Total (without water)57,1124
Polymers57,1124
Non-polymers00
Water724
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
crystal symmetry operation2_556-x,y,-z+11
Unit cell
Length a, b, c (Å)215.360, 38.480, 32.460
Angle α, β, γ (deg.)90.00, 93.11, 90.00
Int Tables number5
Space group name H-MC121

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Components

#1: Protein MAJOR SPERM PROTEIN / MSP


Mass: 14278.107 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Details: ALPHA ISOFORM, PH 4.6 / Source: (gene. exp.) Ascaris suum (pig roundworm) / Cell: SPERM CELL / Gene: ALPHA MSP / Plasmid: PET11D / Species (production host): Escherichia coli / Gene (production host): ALPHA MSP / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21(DE3) / References: UniProt: P27439
#2: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 74 / Source method: isolated from a natural source / Formula: H2O

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION

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Sample preparation

CrystalDensity Matthews: 2.35 Å3/Da / Density % sol: 50 %
Crystal growpH: 4.6 / Details: pH 4.6
Crystal grow
*PLUS
Method: vapor diffusion
Components of the solutions
*PLUS
IDConc.Common nameCrystal-IDSol-ID
111 %(w/v)PEG80001reservoir
22 %satammonium sulfate1reservoir
350 mMammonium acetate1reservoir

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Data collection

Diffraction sourceWavelength: 1.5418
DetectorType: MARRESEARCH / Detector: IMAGE PLATE
RadiationMonochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 1.5418 Å / Relative weight: 1
ReflectionNum. obs: 8858 / % possible obs: 95 % / Observed criterion σ(I): 0 / Redundancy: 1.9 % / Rmerge(I) obs: 0.046
Reflection
*PLUS
Highest resolution: 2.5 Å / Lowest resolution: 21 Å

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Processing

Software
NameClassification
TNTrefinement
MOSFLMdata reduction
RefinementResolution: 2.5→20 Å / Num. reflection obs: 8478 / σ(F): 0 / Stereochemistry target values: TNT
Refinement stepCycle: LAST / Resolution: 2.5→20 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms1857 0 0 74 1931
Refine LS restraints
Refine-IDTypeDev idealWeight
X-RAY DIFFRACTIONt_bond_d0.0130.02
X-RAY DIFFRACTIONt_angle_deg2.683
X-RAY DIFFRACTIONt_dihedral_angle_d21.8315
X-RAY DIFFRACTIONt_incorr_chiral_ct
X-RAY DIFFRACTIONt_pseud_angle
X-RAY DIFFRACTIONt_trig_c_planes
X-RAY DIFFRACTIONt_gen_planes0.0110.02
X-RAY DIFFRACTIONt_it
X-RAY DIFFRACTIONt_nbd
Software
*PLUS
Name: TNT / Classification: refinement
Refinement
*PLUS
Lowest resolution: 21 Å / % reflection Rfree: 5 % / Rfactor obs: 0.195 / Rfactor Rfree: 0.292
Solvent computation
*PLUS
Displacement parameters
*PLUS
Refine LS restraints
*PLUS
Refine-IDTypeDev idealWeight
X-RAY DIFFRACTIONt_dihedral_angle_d
X-RAY DIFFRACTIONt_dihedral_angle_deg21.8315
X-RAY DIFFRACTIONt_plane_restr0.0110.02

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