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Yorodumi- PDB-2moi: 3D NMR structure of the cytoplasmic rhodanese domain of the inner... -
+Open data
-Basic information
Entry | Database: PDB / ID: 2moi | ||||||
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Title | 3D NMR structure of the cytoplasmic rhodanese domain of the inner membrane protein YgaP from Escherichia coli | ||||||
Components | Inner membrane protein YgaP | ||||||
Keywords | MEMBRANE PROTEIN / Rhodanese domain | ||||||
Function / homology | Function and homology information | ||||||
Biological species | Escherichia coli (E. coli) | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
Model details | fewest violations, model 10 | ||||||
Authors | Eichmann, C. / Tzitzilonis, C. / Bordignon, E. / Maslennikov, I. / Choe, S. / Riek, R. | ||||||
Citation | Journal: J.Biol.Chem. / Year: 2014 Title: Solution NMR Structure and Functional Analysis of the Integral Membrane Protein YgaP from Escherichia coli. Authors: Eichmann, C. / Tzitzilonis, C. / Bordignon, E. / Maslennikov, I. / Choe, S. / Riek, R. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2moi.cif.gz | 306.6 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2moi.ent.gz | 250.1 KB | Display | PDB format |
PDBx/mmJSON format | 2moi.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 2moi_validation.pdf.gz | 417.8 KB | Display | wwPDB validaton report |
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Full document | 2moi_full_validation.pdf.gz | 533.8 KB | Display | |
Data in XML | 2moi_validation.xml.gz | 37.4 KB | Display | |
Data in CIF | 2moi_validation.cif.gz | 49.6 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/mo/2moi ftp://data.pdbj.org/pub/pdb/validation_reports/mo/2moi | HTTPS FTP |
-Related structure data
Related structure data | 2molC 2mpnC 2moj C: citing same article (ref.) |
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Similar structure data | |
Other databases |
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 11716.311 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli (E. coli) / Strain: K12 / Gene: b2668, JW2643, ygaP, ygaP b2668 JW2643 / Production host: Escherichia coli (E. coli) / Strain (production host): BL21(DE3) pLysS Star / References: UniProt: P55734 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Details | Contents: 2 mM [U-100% 13C; U-100% 15N] YgaP, 90% H2O/10% D2O Solvent system: 90% H2O/10% D2O |
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Sample | Conc.: 2 mM / Component: entity-1 / Isotopic labeling: [U-100% 13C; U-100% 15N] |
Sample conditions | Ionic strength: 0 / pH: 7.0 / Pressure: ambient / Temperature: 303 K |
-NMR measurement
NMR spectrometer | Type: Bruker Avance / Manufacturer: Bruker / Model: AVANCE / Field strength: 700 MHz |
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-Processing
NMR software |
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Refinement | Method: simulated annealing / Software ordinal: 1 | ||||||||||||
NMR representative | Selection criteria: fewest violations | ||||||||||||
NMR ensemble | Conformer selection criteria: target function / Conformers calculated total number: 100 / Conformers submitted total number: 10 |