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- PDB-2mki: Solution structure of tandem RRM domains of cytoplasmic polyadeny... -
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Open data
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Basic information
Entry | Database: PDB / ID: 2mki | ||||||
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Title | Solution structure of tandem RRM domains of cytoplasmic polyadenylation element binding protein 4 (CPEB4) in complex with RNA | ||||||
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![]() | TRANSLATION REGULATOR/RNA / CPEB4 / Cytoplasmic polyadenylation / RNA recognition motif (RRM) / Protein-RNA Interaction / Translational regulation / TRANSLATION REGULATOR-RNA complex | ||||||
Function / homology | ![]() : / cellular response to decreased oxygen levels / translation factor activity, RNA binding / negative regulation of cytoplasmic translation / cellular response to glucose starvation / ionotropic glutamate receptor signaling pathway / mRNA regulatory element binding translation repressor activity / mRNA 3'-UTR binding / response to ischemia / cellular response to amino acid stimulus ...: / cellular response to decreased oxygen levels / translation factor activity, RNA binding / negative regulation of cytoplasmic translation / cellular response to glucose starvation / ionotropic glutamate receptor signaling pathway / mRNA regulatory element binding translation repressor activity / mRNA 3'-UTR binding / response to ischemia / cellular response to amino acid stimulus / ribosome binding / growth cone / negative regulation of neuron apoptotic process / dendritic spine / postsynaptic density / neuron projection / synapse / dendrite / perinuclear region of cytoplasm / endoplasmic reticulum / RNA binding / nucleus / metal ion binding / cytoplasm Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
Model details | closest to the average, model3 | ||||||
![]() | Afroz, T. / Skrisovska, L. / Belloc, E. / Boixet, J.G. / Mendez, R. / Allain, F.H.-T. | ||||||
![]() | ![]() Title: A fly trap mechanism provides sequence-specific RNA recognition by CPEB proteins Authors: Afroz, T. / Skrisovska, L. / Belloc, E. / Guillen-Boixet, J. / Mendez, R. / Allain, F.H.-T. | ||||||
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 1.3 MB | Display | ![]() |
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PDB format | ![]() | 1.1 MB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 434.6 KB | Display | ![]() |
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Full document | ![]() | 656.9 KB | Display | |
Data in XML | ![]() | 83.5 KB | Display | |
Data in CIF | ![]() | 108.1 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 2mkeC ![]() 2mkhC ![]() 2mkjC ![]() 2mkkC C: citing same article ( |
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Similar structure data | |
Other databases |
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Links
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Assembly
Deposited unit | ![]()
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NMR ensembles |
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Components
#1: Protein | Mass: 22825.979 Da / Num. of mol.: 1 / Fragment: UNP residues 53-255 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
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#2: RNA chain | Mass: 1507.928 Da / Num. of mol.: 1 / Source method: obtained synthetically |
Sequence details | THIS PROTEIN SEQUENCE IS ISOFORM 3 OF CPEB4_HUMAN. |
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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NMR experiment |
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Sample preparation
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Sample |
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Sample conditions | Ionic strength: 0.15 / pH: 7.0 / Pressure: ambient / Temperature: 303 K |
-NMR measurement
NMR spectrometer |
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Processing
NMR software | Name: ![]() Developer: Case, Darden, Cheatham, III, Simmerling, Wang, Duke, Luo, ... and Kollman Classification: refinement |
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Refinement | Method: simulated annealing / Software ordinal: 1 |
NMR representative | Selection criteria: closest to the average |
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 30 / Conformers submitted total number: 20 / Representative conformer: 3 |