+Open data
-Basic information
Entry | Database: PDB / ID: 6ucc | ||||||
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Title | Structure of human PACRG-MEIG1 complex (limited proteolysis) | ||||||
Components |
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Keywords | STRUCTURAL PROTEIN / Microtubule / axoneme / primary cilia / flagella | ||||||
Function / homology | Function and homology information manchette assembly / axonemal B tubule inner sheath / sperm axoneme assembly / axonemal microtubule / manchette / beta-tubulin binding / alpha-tubulin binding / spermatid development / cellular response to unfolded protein / sperm midpiece ...manchette assembly / axonemal B tubule inner sheath / sperm axoneme assembly / axonemal microtubule / manchette / beta-tubulin binding / alpha-tubulin binding / spermatid development / cellular response to unfolded protein / sperm midpiece / Hsp70 protein binding / heat shock protein binding / G protein-coupled receptor binding / Hsp90 protein binding / protein localization / actin binding / cell body / protein-folding chaperone binding / vesicle / neuron projection / ubiquitin protein ligase binding / nucleus / cytosol Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.6 Å | ||||||
Authors | Khan, N. / Croteau, N. / Pelletier, D. / Veyron, S. / Trempe, J.F. | ||||||
Funding support | Canada, 1items
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Citation | Journal: Biorxiv / Year: 2019 Title: Crystal structure of human PACRG in complex with MEIG1 Authors: Khan, N. / Pelletier, D. / Veyron, S. / Croteau, N. / Ichikawa, M. / Black, C. / Khalifa, A.A.Z. / Chaaban, S. / Kurinov, I. / Brouhard, G. / Bui, K.H. / Trempe, J.F. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 6ucc.cif.gz | 127 KB | Display | PDBx/mmCIF format |
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PDB format | pdb6ucc.ent.gz | 92.5 KB | Display | PDB format |
PDBx/mmJSON format | 6ucc.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/uc/6ucc ftp://data.pdbj.org/pub/pdb/validation_reports/uc/6ucc | HTTPS FTP |
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-Related structure data
Related structure data | 6nduSC 6nepC S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
-Protein , 2 types, 2 molecules AB
#1: Protein | Mass: 29352.184 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Details: Generated by limited proteolysis using subtilisin / Source: (gene. exp.) Homo sapiens (human) / Gene: PACRG, GLUP / Plasmid: pGEX-6P1 / Production host: Escherichia coli BL21(DE3) (bacteria) / References: UniProt: Q96M98 |
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#2: Protein | Mass: 11226.584 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Details: Full-length MEIG1 expressed as His-tagged protein, cleaved with the 3C protease, leaving GPLGS at N-terminus Source: (gene. exp.) Homo sapiens (human) / Gene: MEIG1 / Production host: Escherichia coli BL21(DE3) (bacteria) / References: UniProt: Q5JSS6 |
-Non-polymers , 4 types, 24 molecules
#3: Chemical | ChemComp-PEG / |
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#4: Chemical | ChemComp-PG4 / |
#5: Chemical | ChemComp-PO4 / |
#6: Water | ChemComp-HOH / |
-Details
Has ligand of interest | N |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.19 Å3/Da / Density % sol: 43.78 % |
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Crystal grow | Temperature: 283 K / Method: vapor diffusion, sitting drop / Details: 20% glycerol, 0.2M KH2PO4, 20% PEG 8000 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: SYNCHROTRON / Site: CLSI / Beamline: 08ID-1 / Wavelength: 0.9795 Å |
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Jul 7, 2019 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9795 Å / Relative weight: 1 |
Reflection | Resolution: 2.6→47.27 Å / Num. obs: 11793 / % possible obs: 100 % / Redundancy: 12.6 % / Biso Wilson estimate: 54.77 Å2 / CC1/2: 0.996 / Rmerge(I) obs: 0.283 / Rpim(I) all: 0.082 / Rrim(I) all: 0.295 / Χ2: 1 / Net I/σ(I): 9.1 |
Reflection shell | Resolution: 2.6→2.72 Å / Redundancy: 12.7 % / Rmerge(I) obs: 2.868 / Mean I/σ(I) obs: 1.1 / Num. unique obs: 1395 / CC1/2: 0.557 / Rpim(I) all: 0.871 / Rrim(I) all: 3.131 / Χ2: 1 / % possible all: 100 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 6NDU Resolution: 2.6→47.27 Å / SU ML: 0.347 / Cross valid method: FREE R-VALUE / σ(F): 1.33 / Phase error: 27.8609 Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 58.93 Å2 | ||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.6→47.27 Å
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Refine LS restraints |
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LS refinement shell |
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