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Yorodumi- PDB-2mig: Solution structure of the CLAVATA-like encoded peptide of Meloido... -
+Open data
-Basic information
Entry | Database: PDB / ID: 2mig | ||||||
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Title | Solution structure of the CLAVATA-like encoded peptide of Meloidogyne hapla - MhCLE5 | ||||||
Components | CLAVATA-like encoded peptide of Meloidogyne hapla - MhCLE5 | ||||||
Keywords | UNKNOWN FUNCTION / CLE / CLAVATA | ||||||
Biological species | Meloidogyne hapla (invertebrata) | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
Authors | Bobay, B.G. / DiGennaro, P.M. / Bird, D.M. | ||||||
Citation | Journal: To be Published Title: Inferring function of CLE peptides from high resolution tertiary structures Authors: DiGennaro, P.G. / Bobay, B.M. / Bird, D.M. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2mig.cif.gz | 39.9 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2mig.ent.gz | 26.8 KB | Display | PDB format |
PDBx/mmJSON format | 2mig.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/mi/2mig ftp://data.pdbj.org/pub/pdb/validation_reports/mi/2mig | HTTPS FTP |
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-Related structure data
Related structure data | 2midC 2mieC 2mifC 2mihC C: citing same article (ref.) |
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Similar structure data | |
Other databases |
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein/peptide | Mass: 1328.497 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) Meloidogyne hapla (invertebrata) |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Details | Contents: 4 mg/mL peptide, 137 mM sodium chloride, 2.7 mM potassium chloride, 10 mM sodium phosphate, 1.8 mM potassium phosphate, 90% H2O/10% D2O Solvent system: 90% H2O/10% D2O | ||||||||||||||||||
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Sample |
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Sample conditions | Ionic strength: 140 / pH: 7.0 / Pressure: ambient / Temperature: 298 K |
-NMR measurement
NMR spectrometer | Type: Bruker Avance / Manufacturer: Bruker / Model: AVANCE / Field strength: 700 MHz |
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-Processing
NMR software |
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Refinement | Method: simulated annealing / Software ordinal: 1 | |||||||||||||||||||||
NMR representative | Selection criteria: lowest energy | |||||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 20 / Conformers submitted total number: 10 |