[English] 日本語
Yorodumi- PDB-2mie: Solution structure of the CLAVATA encoded peptide of Arabidopsis ... -
+Open data
-Basic information
Entry | Database: PDB / ID: 2mie | ||||||
---|---|---|---|---|---|---|---|
Title | Solution structure of the CLAVATA encoded peptide of Arabidopsis thaliana - AtCLE44 | ||||||
Components | CLAVATA3/ESR (CLE)-related protein 44 | ||||||
Keywords | UNKNOWN FUNCTION / CLE / CLAVATA | ||||||
Function / homology | Function and homology information procambium histogenesis / phloem development / xylem development / axillary shoot meristem initiation / maintenance of root meristem identity / cell-cell signaling involved in cell fate commitment / phloem or xylem histogenesis / secondary shoot formation / apoplast / receptor serine/threonine kinase binding ...procambium histogenesis / phloem development / xylem development / axillary shoot meristem initiation / maintenance of root meristem identity / cell-cell signaling involved in cell fate commitment / phloem or xylem histogenesis / secondary shoot formation / apoplast / receptor serine/threonine kinase binding / regulation of cell differentiation / cell division Similarity search - Function | ||||||
Biological species | Arabidopsis thaliana (thale cress) | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
Authors | Bobay, B.G. / DiGennaro, P.M. / Bird, D.M. | ||||||
Citation | Journal: To be Published Title: Inferring function of CLE peptides from high resolution tertiary structures Authors: DiGennaro, P.M. / Bobay, B.G. / Bird, D.M. | ||||||
History |
|
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
---|
-Downloads & links
-Download
PDBx/mmCIF format | 2mie.cif.gz | 34.5 KB | Display | PDBx/mmCIF format |
---|---|---|---|---|
PDB format | pdb2mie.ent.gz | 23.6 KB | Display | PDB format |
PDBx/mmJSON format | 2mie.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 2mie_validation.pdf.gz | 417.5 KB | Display | wwPDB validaton report |
---|---|---|---|---|
Full document | 2mie_full_validation.pdf.gz | 459.7 KB | Display | |
Data in XML | 2mie_validation.xml.gz | 8.2 KB | Display | |
Data in CIF | 2mie_validation.cif.gz | 9.1 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/mi/2mie ftp://data.pdbj.org/pub/pdb/validation_reports/mi/2mie | HTTPS FTP |
-Related structure data
Related structure data | 2midC 2mifC 2migC 2mihC C: citing same article (ref.) |
---|---|
Similar structure data | |
Other databases |
-Links
-Assembly
Deposited unit |
| |||||||||
---|---|---|---|---|---|---|---|---|---|---|
1 |
| |||||||||
NMR ensembles |
|
-Components
#1: Protein/peptide | Mass: 1248.322 Da / Num. of mol.: 1 / Fragment: UNP residues 101-112 / Source method: obtained synthetically / Source: (synth.) Arabidopsis thaliana (thale cress) / References: UniProt: Q941C5, UniProt: Q84W98*PLUS |
---|
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
NMR experiment |
|
-Sample preparation
Details | Contents: 4 mg/mL peptide, 2.7 mM potassium chloride, 1.8 mM potassium phosphate, 137 mM sodium chloride, 10 mM sodium phosphate, 90% H2O/10% D2O Solvent system: 90% H2O/10% D2O | ||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Sample |
| ||||||||||||||||||
Sample conditions | Ionic strength: 140 / pH: 7.0 / Pressure: ambient / Temperature: 298 K |
-NMR measurement
NMR spectrometer | Type: Bruker Avance / Manufacturer: Bruker / Model: AVANCE / Field strength: 700 MHz |
---|
-Processing
NMR software |
| |||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method: simulated annealing / Software ordinal: 1 | |||||||||||||||||||||
NMR representative | Selection criteria: lowest energy | |||||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 20 / Conformers submitted total number: 10 |