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- PDB-2mfm: Solution structure of the C-terminally encoded peptide of the pla... -
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Open data
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Basic information
Entry | Database: PDB / ID: 2mfm | ||||||
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Title | Solution structure of the C-terminally encoded peptide of the plant parasitic nematode Meloidogyne hapla - CEP11 | ||||||
![]() | CEP11 | ||||||
![]() | HORMONE / CEP / Meloidogyne / root-knot nematode / CLE | ||||||
Function / homology | nitrate import / regulation of root development / regulation of leaf morphogenesis / hormone activity / extracellular region / Putative encoded peptide![]() | ||||||
Biological species | ![]() | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
Model details | lowest energy, model1 | ||||||
![]() | Bobay, B.G. / DiGennaro, P. / Bird, D.M. | ||||||
![]() | ![]() Title: Solution NMR studies of the plant peptide hormone CEP inform function. Authors: Bobay, B.G. / Digennaro, P. / Scholl, E. / Imin, N. / Djordjevic, M.A. / McK Bird, D. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 41.9 KB | Display | ![]() |
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PDB format | ![]() | 32 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 395.8 KB | Display | ![]() |
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Full document | ![]() | 422.2 KB | Display | |
Data in XML | ![]() | 5.5 KB | Display | |
Data in CIF | ![]() | 7.1 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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NMR ensembles |
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Components
#1: Protein/peptide | Mass: 1522.644 Da / Num. of mol.: 1 / Source method: obtained synthetically Details: Synthetic peptide with modification of hydroxy-proline at positions 4 and 11 Source: (synth.) ![]() |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||
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NMR experiment |
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NMR details | Text: NOESY collected at 50 and 300 ms ; TOCSY collected at 30 and 80 ms |
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Sample preparation
Details | Contents: 4 mg/mL protein, 10 uM DSS, 90% H2O/10% D2O / Solvent system: 90% H2O/10% D2O | |||||||||
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Sample |
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Sample conditions | Ionic strength: 0 / pH: 7.0 / Pressure: ambient / Temperature: 298 K |
-NMR measurement
NMR spectrometer | Type: Bruker Avance / Manufacturer: Bruker / Model: Avance / Field strength: 700 MHz |
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Processing
NMR software |
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Refinement | Method: simulated annealing / Software ordinal: 1 | |||||||||||||||||||||
NMR representative | Selection criteria: lowest energy | |||||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 10 / Conformers submitted total number: 10 |