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Yorodumi- PDB-7jhf: Solution NMR structure of protonectin-F, a derived peptide from wasp -
+Open data
-Basic information
Entry | Database: PDB / ID: 7jhf | ||||||
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Title | Solution NMR structure of protonectin-F, a derived peptide from wasp | ||||||
Components | Protonectin-F derived peptide ILE-PHE-GLY-THR-ILE-LEU-GLY-PHE-LEU-LYS-GLY-LEU-NH2 | ||||||
Keywords | ANTIMICROBIAL PROTEIN / alpha-helical / antimicrobial / non-haemolytic | ||||||
Biological species | Parachartergus fraternus (insect) | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
Authors | Muller, J.A.I. / Craik, D.J. / Koehbach, J. | ||||||
Citation | Journal: Chembiochem / Year: 2021 Title: Antimicrobial and Anticancer Properties of Synthetic Peptides Derived from the Wasp Parachartergus fraternus. Authors: Muller, J.A.I. / Lawrence, N. / Chan, L.Y. / Harvey, P.J. / Elliott, A.G. / Blaskovich, M.A.T. / Goncalves, J.C. / Galante, P. / Mortari, M.R. / Toffoli-Kadri, M.C. / Koehbach, J. / Craik, D.J. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 7jhf.cif.gz | 83.8 KB | Display | PDBx/mmCIF format |
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PDB format | pdb7jhf.ent.gz | 61.4 KB | Display | PDB format |
PDBx/mmJSON format | 7jhf.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 7jhf_validation.pdf.gz | 414.3 KB | Display | wwPDB validaton report |
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Full document | 7jhf_full_validation.pdf.gz | 491.2 KB | Display | |
Data in XML | 7jhf_validation.xml.gz | 7.4 KB | Display | |
Data in CIF | 7jhf_validation.cif.gz | 10.5 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/jh/7jhf ftp://data.pdbj.org/pub/pdb/validation_reports/jh/7jhf | HTTPS FTP |
-Related structure data
Related structure data | 7jgxC 7jgyC C: citing same article (ref.) |
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Similar structure data | |
Other databases |
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-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein/peptide | Mass: 1277.599 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) Parachartergus fraternus (insect) |
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Has ligand of interest | N |
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Details | Type: solution / Contents: 1 mM peptide, 70% H20/30% TFE / Label: sample_1 / Solvent system: 70% H20/30% TFE |
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Sample | Conc.: 1 mM / Component: peptide / Isotopic labeling: natural abundance |
Sample conditions | Ionic strength units: Not defined / Label: condition_1 / pH: 4.5 / Pressure: 1 atm / Temperature: 298 K |
-NMR measurement
NMR spectrometer | Type: Bruker AVANCE III HD / Manufacturer: Bruker / Model: AVANCE III HD / Field strength: 600 MHz |
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-Processing
NMR software |
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Refinement | Method: simulated annealing / Software ordinal: 1 | ||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 50 / Conformers submitted total number: 20 |