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Yorodumi- PDB-2mfm: Solution structure of the C-terminally encoded peptide of the pla... -
+Open data
-Basic information
Entry | Database: PDB / ID: 2mfm | ||||||
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Title | Solution structure of the C-terminally encoded peptide of the plant parasitic nematode Meloidogyne hapla - CEP11 | ||||||
Components | CEP11 | ||||||
Keywords | HORMONE / CEP / Meloidogyne / root-knot nematode / CLE | ||||||
Function / homology | nitrate import / regulation of root development / regulation of leaf morphogenesis / hormone activity / extracellular region / Putative encoded peptide Function and homology information | ||||||
Biological species | Meloidogyne hapla (invertebrata) | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
Model details | lowest energy, model1 | ||||||
Authors | Bobay, B.G. / DiGennaro, P. / Bird, D.M. | ||||||
Citation | Journal: Febs Lett. / Year: 2013 Title: Solution NMR studies of the plant peptide hormone CEP inform function. Authors: Bobay, B.G. / Digennaro, P. / Scholl, E. / Imin, N. / Djordjevic, M.A. / McK Bird, D. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2mfm.cif.gz | 41.9 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2mfm.ent.gz | 32 KB | Display | PDB format |
PDBx/mmJSON format | 2mfm.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/mf/2mfm ftp://data.pdbj.org/pub/pdb/validation_reports/mf/2mfm | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein/peptide | Mass: 1522.644 Da / Num. of mol.: 1 / Source method: obtained synthetically Details: Synthetic peptide with modification of hydroxy-proline at positions 4 and 11 Source: (synth.) Meloidogyne hapla (invertebrata) / References: UniProt: G7K427*PLUS |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||
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NMR experiment |
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NMR details | Text: NOESY collected at 50 and 300 ms ; TOCSY collected at 30 and 80 ms |
-Sample preparation
Details | Contents: 4 mg/mL protein, 10 uM DSS, 90% H2O/10% D2O / Solvent system: 90% H2O/10% D2O | |||||||||
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Sample |
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Sample conditions | Ionic strength: 0 / pH: 7.0 / Pressure: ambient / Temperature: 298 K |
-NMR measurement
NMR spectrometer | Type: Bruker Avance / Manufacturer: Bruker / Model: Avance / Field strength: 700 MHz |
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-Processing
NMR software |
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Refinement | Method: simulated annealing / Software ordinal: 1 | |||||||||||||||||||||
NMR representative | Selection criteria: lowest energy | |||||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 10 / Conformers submitted total number: 10 |