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Open data
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Basic information
Entry | Database: PDB / ID: 2mel | ||||||
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Title | NMR solution structure of the GS-TAMAPIN MUTATION R7A | ||||||
![]() | Potassium channel toxin alpha-KTx 5.4 | ||||||
![]() | TOXIN / Scorpion toxin / tamapin / alpha KTx5.4 mutant R7A | ||||||
Function / homology | Scorpion short toxins signature. / Scorpion short chain toxin, potassium channel inhibitor / Scorpion short toxin, BmKK2 / Knottin, scorpion toxin-like superfamily / ion channel inhibitor activity / potassium channel regulator activity / toxin activity / extracellular region / Potassium channel toxin alpha-KTx 5.4![]() | ||||||
Biological species | ![]() | ||||||
Method | SOLUTION NMR / molecular dynamics | ||||||
![]() | del Rio-Portilla, F. / Ramirez-Cordero, B. | ||||||
![]() | ![]() Title: Cytotoxicity of recombinant tamapin and related toxin-like peptides on model cell lines. Authors: Ramirez-Cordero, B. / Toledano, Y. / Cano-Sanchez, P. / Hernandez-Lopez, R. / Flores-Solis, D. / Saucedo-Yanez, A.L. / Chavez-Uribe, I. / Brieba, L.G. / Del Rio-Portilla, F. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 183.4 KB | Display | ![]() |
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PDB format | ![]() | 160.9 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 360.8 KB | Display | ![]() |
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Full document | ![]() | 449.3 KB | Display | |
Data in XML | ![]() | 11.9 KB | Display | |
Data in CIF | ![]() | 19.9 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 2ky3C ![]() 2lu9C ![]() 2me7C ![]() 2menC ![]() 2meoC C: citing same article ( |
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Similar structure data | |
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Links
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Assembly
Deposited unit | ![]()
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NMR ensembles |
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Components
#1: Protein/peptide | Mass: 3528.199 Da / Num. of mol.: 1 / Mutation: R9A Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Plasmid: modified pEt32a / Production host: ![]() ![]() |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||
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NMR experiment |
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Sample preparation
Details | Contents: 2.5 mM protein, 95% H2O/5% D2O / Solvent system: 95% H2O/5% D2O |
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Sample | Conc.: 2.5 mM / Component: entity-1 |
Sample conditions | pH: 3.35 / Pressure: ambient / Temperature: 298 K |
-NMR measurement
NMR spectrometer | Type: Varian INOVA / Manufacturer: Varian / Model: INOVA / Field strength: 500 MHz |
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Processing
NMR software |
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Refinement | Method: molecular dynamics / Software ordinal: 1 | ||||||||||||||||||||
NMR constraints | NOE constraints total: 524 / NOE long range total count: 97 / NOE medium range total count: 115 | ||||||||||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the least restraint violations Conformers calculated total number: 200 / Conformers submitted total number: 20 |