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Open data
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Basic information
Entry | Database: PDB / ID: 2lu9 | ||||||
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Title | NMR solution structure of recombinant Tamapin | ||||||
![]() | Potassium channel toxin alpha-KTx 5.4 | ||||||
![]() | TOXIN / CS alpha beta | ||||||
Function / homology | Scorpion short toxins signature. / Scorpion short chain toxin, potassium channel inhibitor / Scorpion short toxin, BmKK2 / Knottin, scorpion toxin-like superfamily / ion channel inhibitor activity / potassium channel regulator activity / toxin activity / extracellular region / Potassium channel toxin alpha-KTx 5.4![]() | ||||||
Biological species | ![]() | ||||||
Method | SOLUTION NMR / molecular dynamics, simulated annealing | ||||||
![]() | del Rio-Portilla, F. / Ramirez-Cordero, B. | ||||||
![]() | ![]() Title: Cytotoxicity of recombinant tamapin and related toxin-like peptides on model cell lines. Authors: Ramirez-Cordero, B. / Toledano, Y. / Cano-Sanchez, P. / Hernandez-Lopez, R. / Flores-Solis, D. / Saucedo-Yanez, A.L. / Chavez-Uribe, I. / Brieba, L.G. / Del Rio-Portilla, F. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 164.8 KB | Display | ![]() |
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PDB format | ![]() | 145.3 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 365.9 KB | Display | ![]() |
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Full document | ![]() | 436.7 KB | Display | |
Data in XML | ![]() | 10.6 KB | Display | |
Data in CIF | ![]() | 17.3 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 2ky3C ![]() 2me7C ![]() 2melC ![]() 2menC ![]() 2meoC ![]() 2lu8 C: citing same article ( |
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Similar structure data | |
Other databases |
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Links
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Assembly
Deposited unit | ![]()
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NMR ensembles |
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Components
#1: Protein/peptide | Mass: 3470.186 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() References: UniProt: P59869 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||
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NMR experiment |
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Sample preparation
Details | Contents: 0.8 mM recombinant-tamapin, 95% H2O, 5% D2O / Solvent system: 95% H2O/5% D2O |
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Sample | Conc.: 0.8 mM / Component: recombinant-tamapin-1 |
Sample conditions | pH: 6.0 / Pressure: ambient / Temperature: 298 K |
-NMR measurement
NMR spectrometer | Type: Varian Inova / Manufacturer: Varian / Model: INOVA / Field strength: 500 MHz |
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Processing
NMR software | Name: ![]() Developer: Case, Darden, Cheatham, III, Simmerling, Wang, Duke, Luo, ... and Kollman Classification: refinement |
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Refinement | Method: molecular dynamics, simulated annealing / Software ordinal: 1 |
NMR representative | Selection criteria: fewest violations |
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 200 / Conformers submitted total number: 18 |