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Yorodumi- PDB-2m6l: Solution structure of the Escherichia coli holo ferric enterobact... -
+Open data
-Basic information
Entry | Database: PDB / ID: 2m6l | ||||||
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Title | Solution structure of the Escherichia coli holo ferric enterobactin binding protein | ||||||
Components | Ferrienterobactin-binding periplasmic protein | ||||||
Keywords | TRANSPORT PROTEIN / periplasmic / siderophore | ||||||
Function / homology | Function and homology information ferric-enterobactin import into cell / iron import into cell / ATP-binding cassette (ABC) transporter complex, substrate-binding subunit-containing / outer membrane-bounded periplasmic space / DNA damage response / membrane Similarity search - Function | ||||||
Biological species | Escherichia coli (E. coli) | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
Model details | lowest energy, model1 | ||||||
Authors | Chu, B.C.H. / Otten, R. / Krewulak, K.D. / Mulder, F.A.A. / Vogel, H.J. | ||||||
Citation | Journal: J.Biol.Chem. / Year: 2014 Title: The solution structure, binding properties, and dynamics of the bacterial siderophore-binding protein FepB. Authors: Chu, B.C. / Otten, R. / Krewulak, K.D. / Mulder, F.A. / Vogel, H.J. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2m6l.cif.gz | 2.5 MB | Display | PDBx/mmCIF format |
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PDB format | pdb2m6l.ent.gz | 2.1 MB | Display | PDB format |
PDBx/mmJSON format | 2m6l.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 2m6l_validation.pdf.gz | 419.5 KB | Display | wwPDB validaton report |
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Full document | 2m6l_full_validation.pdf.gz | 641.4 KB | Display | |
Data in XML | 2m6l_validation.xml.gz | 194.6 KB | Display | |
Data in CIF | 2m6l_validation.cif.gz | 234.4 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/m6/2m6l ftp://data.pdbj.org/pub/pdb/validation_reports/m6/2m6l | HTTPS FTP |
-Related structure data
Related structure data | 2m6kC C: citing same article (ref.) |
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Similar structure data | |
Other databases |
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-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 34370.480 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli (E. coli) / Strain: K12 / Gene: b0592, fepB, JW0584 / Production host: Escherichia coli (E. coli) / Strain (production host): BL21 / References: UniProt: P0AEL6 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Details |
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Sample conditions | Ionic strength: 0 / pH: 6.4 / Pressure: ambient / Temperature: 298 K |
-NMR measurement
NMR spectrometer | Type: Bruker Avance / Manufacturer: Bruker / Model: AVANCE / Field strength: 700 MHz |
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-Processing
NMR software | Name: X-PLOR NIH / Classification: refinement |
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Refinement | Method: simulated annealing / Software ordinal: 1 |
NMR representative | Selection criteria: lowest energy |
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 100 / Conformers submitted total number: 30 |