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- SASDB62: Death associated protein kinase (D220K mutant) (hDAPK2 D220K) -

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Basic information

Entry
Database: SASBDB / ID: SASDB62
SampleDeath associated protein kinase (D220K mutant)
  • Death associated protein kinase (D220K mutant) (protein), hDAPK2 D220K, Homo sapiens
Biological speciesHomo sapiens (human)
CitationYear: 2016
Title: Death-Associated Protein Kinase Activity Is Regulated by Coupled Calcium/Calmodulin Binding to Two Distinct Sites
Authors: Simon B / Huart A / Temmerman K / Vahokoski J / Mertens H / Komadina D / Hoffmann J / Yumerefendi H / Svergun D / Kursula P / Schultz C / McCarthy A / Hart D
Contact author
  • Haydyn Mertens (EMBL-Hamburg, European Molecular Biology Laboratory (EMBL) - Hamburg outstation, Notkestraße 85, Geb. 25A, 22607 Hamburg, Deutschland, Germany)

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

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Models

Model #386
Type: atomic / Radius of dummy atoms: 1.90 A / Comment: Dimer component PDB 2A2A:AB / Chi-square value: 1.01
Search similar-shape structures of this assembly by Omokage search (details)
Model #387
Type: atomic / Radius of dummy atoms: 1.90 A / Comment: Monomer component PDB 2A2A:A / Chi-square value: 1.01
Search similar-shape structures of this assembly by Omokage search (details)

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Sample

SampleName: Death associated protein kinase (D220K mutant) / Dry vol: 45000 / Specimen concentration: 2 mg/ml
BufferName: 50 mM HEPES 250 mM NaCl 5mM CaCl2 0.25 mM TCEP 5% (v/v) glycerol
Concentration: 50.00 mM / pH: 7.5
Composition: 250 mM NaCl, 5mM CaCl2, 0.25 mM TCEP, 5% [v/v] glycerol
Entity #252Name: hDAPK2 D220K / Type: protein / Description: Death associated protein kinase (D220K mutant) / Formula weight: 37.3 / Source: Homo sapiens
Sequence: GPMSEPFKQQ KVEDFYDIGE ELGSGQFAIV KKCREKSTGL EYAAKFIKKR QSRASRRGVS REEIEREVSI LRQVLHHNVI TLHDVYENRT DVVLILELVS GGELFDFLAQ KESLSEEEAT SFIKQILDGV NYLHTKKIAH FDLKPENIML LDKNIPIPHI KLIDFGLAHE ...Sequence:
GPMSEPFKQQ KVEDFYDIGE ELGSGQFAIV KKCREKSTGL EYAAKFIKKR QSRASRRGVS REEIEREVSI LRQVLHHNVI TLHDVYENRT DVVLILELVS GGELFDFLAQ KESLSEEEAT SFIKQILDGV NYLHTKKIAH FDLKPENIML LDKNIPIPHI KLIDFGLAHE IEDGVEFKNI FGTPEFVAPE IVNYEPLGLE ADMWSIGVIT YILLSGASPF LGKTKQETLA NITSVSYDFD EEFFSHTSEL AKDFIRKLLV KETRKRLTIQ EALRHPWITP VDNQQAMVRR ESVVNLENFR KQYVRRRWKL SFSIVSLCNH LTR

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Experimental information

BeamInstrument name: PETRA III P12 / City: Hamburg / 国: Germany / Type of source: X-ray synchrotron / Wavelength: 0.12 Å / Dist. spec. to detc.: 3.1 mm
DetectorName: Pilatus 2M
Scan
Title: hDAPK2 D220K mutant / Measurement date: Dec 18, 2013 / Storage temperature: 10 °C / Cell temperature: 10 °C / Exposure time: 0.05 sec. / Number of frames: 20 / Unit: 1/nm /
MinMax
Q0.0275 4.4504
Distance distribution function P(R)
Sofotware P(R): GNOM 5.0 / Number of points: 1156 /
MinMax
Q0.143312 3.18224
P(R) point1 1156
R0 9
Result
Type of curve: single_conc
Comments: Characterisation of effect of mutation D220K of hDAPK2 on dimer formation
ExperimentalPorodEstimated
MW37.4 kDa52 kDa-
Volume-89 nm345000

P(R)Guinier
Forward scattering, I0722 717
Radius of gyration, Rg2.6 nm2.5 nm

MinMax
D-90
Guinier point45 159

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