SASDB72: Death associated protein kinase (Basic Loop mutant)

Basic information

• Entry: Database: SASBDB / ID: SASDB72

Sample

Death associated protein kinase (Basic Loop mutant)

• Death associated protein kinase (Basic Loop mutant) (protein), hDAPK2 BL mutant, Homo sapiens

• Biological species: Homo sapiens (human)
• Citation: Year: 2016; Title: Death-Associated Protein Kinase Activity Is Regulated by Coupled Calcium/Calmodulin Binding to Two Distinct Sites; Authors: Simon B / Huart A / Temmerman K / Vahokoski J / Mertens H / Komadina D / Hoffmann J / Yumerefendi H / Svergun D / Kursula P / Schultz C / McCarthy A / Hart D

Contact author

• Haydyn Mertens (EMBL-Hamburg, European Molecular Biology Laboratory (EMBL) - Hamburg outstation, Notkestraße 85, Geb. 25A, 22607 Hamburg, Deutschland, Germany)

Models

• Model #388: ; Type: atomic / Radius of dummy atoms: 1.90 A / Comment: Dimer component PDB 2A2A:AB / Chi-square value: 0.97; Search similar-shape structures of this assembly by Omokage search (details)
• Model #389: ; Type: atomic / Radius of dummy atoms: 1.90 A / Comment: Monomer component PDB 2A2A:A / Chi-square value: 0.97; Search similar-shape structures of this assembly by Omokage search (details)

Sample

• Sample: Name: Death associated protein kinase (Basic Loop mutant) / Dry vol: 45000 / Specimen concentration: 2 mg/ml
• Buffer: Name: 50 mM HEPES 250 mM NaCl 5mM CaCl2 0.25 mM TCEP 5% (v/v) glycerol; Concentration: 50.00 mM / pH: 7.5 ; Composition: 250 mM NaCl, 5mM CaCl2, 0.25 mM TCEP, 5% [v/v] glycerol

Entity #253

Name: hDAPK2 BL mutant / Type: protein
Description: Death associated protein kinase (Basic Loop mutant)
Formula weight: 37.3 / Source: Homo sapiens
Sequence:

GPMSEPFKQQ KVEDFYDIGE ELGSGQFAIV KKCREKSTGL EYAAKFIKKE QSEASEEGVS REEIEREVSI LRQVLHHNVI TLHDVYENRT DVVLILELVS GGELFDFLAQ KESLSEEEAT SFIKQILDGV NYLHTKKIAH FDLKPENIML LDKNIPIPHI KLIDFGLAHE IEDGVEFKNI FGTPEFVAPE IVNYEPLGLE ADMWSIGVIT YILLSGASPF LGDTKQETLA NITSVSYDFD EEFFSHTSEL AKDFIRKLLV KETRKRLTIQ EALRHPWITP VDNQQAMVRR ESVVNLENFR KQYVRRRWKL SFSIVSLCNH LTR

Experimental information

• Beam: Instrument name: PETRA III P12 / City: Hamburg / 国: Germany / Type of source: X-ray synchrotron / Wavelength: 0.12 Å / Dist. spec. to detc.: 3.1 mm
• Detector: Name: Pilatus 2M

Scan

Title: hDAPK2 Basic Loop mutant / Measurement date: Dec 18, 2013 / Storage temperature: 10 °C / Cell temperature: 10 °C / Exposure time: 0.05 sec. / Number of frames: 20 / Unit: 1/nm /

	Min	Max
Q	0.0275	4.4504

Distance distribution function P(R)

Sofotware P(R): GNOM 5.0 / Number of points: 1153 /

	Min	Max
Q	0.151205	3.18224
P(R) point	1	1153
R	0	7.5

Result

Type of curve: single_conc
Comments: Characterisation of mutation of basic loop residues (R47E, R50E, R53E, R54E) on dimer formation of hDAPK2

	Experimental	Porod	Estimated
MW	32 kDa	46 kDa	-
Volume	-	78 nm3	45000

	P(R)	Guinier
Forward scattering, I0	617	623
Radius of gyration, Rg	2.3 nm	2.4 nm

	Min	Max
D	-	75
Guinier point	48	189