- PDB-2m4n: Solution structure of the putative Ras interaction domain of AFD-... -
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基本情報
登録情報
データベース: PDB / ID: 2m4n
タイトル
Solution structure of the putative Ras interaction domain of AFD-1, isoform a from Caenorhabditis elegans
要素
Protein AFD-1, isoform a
キーワード
UNKNOWN FUNCTION / STRUCTURAL GENOMICS / THIOREDOXIN-LIKE / New York Structural Genomics Research Consortium / NYSGRC / Assembly / Dynamics and Evolution of Cell-Cell and Cell-Matrix Adhesions / CELLMAT / PSI-Biology
機能・相同性
機能・相同性情報
cell adhesion molecule binding / adherens junction / regulation of protein localization / cell adhesion / signal transduction 類似検索 - 分子機能
Text: All 3D experiments were acquired using 30% non-uniform sampling using the MDDNMR approach
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試料調製
詳細
Solution-ID
内容
溶媒系
1
1.0 mM [U-100% 13C; U-100% 15N] putative Ras interaction domain of AFD, isoform a, 20 mM sodium phosphate, 50 mM sodium chloride, 1 mM DTT, 0.1 mM EDTA, 90% H2O, 10% D2O
90% H2O/10% D2O
2
1.0 mM [U-100% 13C; U-100% 15N] putative Ras interaction domain of AFD, isoform a, 20 mM sodium phosphate, 50 mM sodium chloride, 1 mM DTT, 0.1 mM EDTA, 100% D2O
100% D2O
試料
濃度 (mg/ml)
構成要素
Isotopic labeling
Solution-ID
1.0mM
putative Ras interaction domain of AFD-1, isoform a-1
[U-100% 13C; U-100% 15N]
1
20mM
sodium phosphate-2
1
50mM
sodium chloride-3
1
1mM
DTT-4
1
0.1mM
EDTA-5
1
1.0mM
putative Ras interaction domain of AFD-1, isoform a-6
手法: simulating annealing / ソフトェア番号: 1 / 詳細: Refinement in a box of water
NMR constraints
NOE constraints total: 1754 / NOE intraresidue total count: 513 / NOE long range total count: 577 / NOE medium range total count: 215 / NOE sequential total count: 361 / Protein other angle constraints total count: 27 / Protein phi angle constraints total count: 46 / Protein psi angle constraints total count: 46
代表構造
選択基準: lowest energy
NMRアンサンブル
コンフォーマー選択の基準: 20 structures for lowest energy 計算したコンフォーマーの数: 100 / 登録したコンフォーマーの数: 20