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Yorodumi- PDB-2lxk: Backbone 1H, 13C, and 15N Chemical Shift Assignments for cold sho... -
+Open data
-Basic information
Entry | Database: PDB / ID: 2lxk | ||||||
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Title | Backbone 1H, 13C, and 15N Chemical Shift Assignments for cold shock protein, LmCsp | ||||||
Components | Cold shock-like protein CspLA | ||||||
Keywords | TRANSCRIPTION / Protein / Nucleic Acids | ||||||
Function / homology | Function and homology information mRNA stabilization / regulation of gene expression / nucleic acid binding / DNA binding / cytoplasm Similarity search - Function | ||||||
Biological species | Listeria monocytogenes (bacteria) | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
Model details | lowest energy, model1 | ||||||
Authors | Lee, J. / Jeong, K. / Kim, Y. | ||||||
Citation | Journal: Biochemistry / Year: 2013 Title: Structural and Dynamic Features of Cold-Shock Proteins of Listeriamonocytogenes, a Psychrophilic Bacterium Authors: Lee, J. / Jeong, K. / Jin, B. / Ryu, K. / Kim, E.H. / Ahn, J.H. / Kim, Y. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2lxk.cif.gz | 445.7 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2lxk.ent.gz | 376.9 KB | Display | PDB format |
PDBx/mmJSON format | 2lxk.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/lx/2lxk ftp://data.pdbj.org/pub/pdb/validation_reports/lx/2lxk | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 7272.875 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Listeria monocytogenes (bacteria) / Strain: ATCC BAA-679 / EGD-e / Gene: cspLA, cspA, cspL, lmo1364 / Production host: Escherichia coli (E. coli) / References: UniProt: P0A355 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Details | Contents: 0.8mM [U-13C; U-15N] entity-1, 50mM potassium phosphate-2, 0.1mM EDTA-3, 100mM potassium chloride-4, 0.004v/v DSS-5, 90% H2O/10% D2O Solvent system: 90% H2O/10% D2O | ||||||||||||||||||||||||
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Sample |
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Sample conditions | pH: 6 / Pressure: ambient / Temperature: 298 K |
-NMR measurement
NMR spectrometer |
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-Processing
NMR software |
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Refinement | Method: simulated annealing / Software ordinal: 1 / Details: CNS water refinement | ||||||||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||||||||
NMR ensemble | Conformer selection criteria: target function / Conformers calculated total number: 100 / Conformers submitted total number: 20 / Representative conformer: 1 |