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Yorodumi- PDB-3nla: NMR STRUCTURE OF THE N-TERMINAL DOMAIN WITH A LINKER PORTION OF A... -
+Open data
-Basic information
Entry | Database: PDB / ID: 3nla | ||||||
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Title | NMR STRUCTURE OF THE N-TERMINAL DOMAIN WITH A LINKER PORTION OF ANTARCTIC EEL POUT ANTIFREEZE PROTEIN RD3, 40 STRUCTURES | ||||||
Components | ANTIFREEZE PROTEIN RD3 TYPE III | ||||||
Keywords | ANTIFREEZE / ANTIFREEZE PROTEIN / THERMAL HYSTERESIS PROTEIN / ICE BINDING PROTEIN | ||||||
Function / homology | Function and homology information | ||||||
Biological species | Lycodichthys dearborni (Antarctic eel pout) | ||||||
Method | SOLUTION NMR / SA | ||||||
Authors | Miura, K. / Ohgiya, S. / Hoshino, T. / Nemoto, N. / Hikichi, K. / Tsuda, S. | ||||||
Citation | Journal: Nature / Year: 1996 Title: Structural basis for the binding of a globular antifreeze protein to ice. Authors: Jia, Z. / DeLuca, C.I. / Chao, H. / Davies, P.L. #1: Journal: Nature / Year: 1996 Title: Structural Basis for the Binding of a Globular Antifreeze Protein to Ice Authors: Jia, Z. / Deluca, C.I. / Chao, H. / Davies, P.L. #2: Journal: Structure / Year: 1996 Title: Refined Solution Structure of Type III Antifreeze Protein: Hydrophobic Groups May be Involved in the Energetics of the Protein-Ice Interaction Authors: Sonnichsen, F.D. / Deluca, C.I. / Davies, P.L. / Sykes, B.D. #3: Journal: Biochim.Biophys.Acta / Year: 1995 Title: Antifreeze Peptide Heterogeneity in an Antarctic Eel Pout Includes an Unusually Large Major Variant Comprised of Two 7 kDa Type III Afps Linked in Tandem Authors: Wang, X. / Devries, A.L. / Cheng, C.H. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 3nla.cif.gz | 847 KB | Display | PDBx/mmCIF format |
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PDB format | pdb3nla.ent.gz | 714.1 KB | Display | PDB format |
PDBx/mmJSON format | 3nla.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 3nla_validation.pdf.gz | 342.6 KB | Display | wwPDB validaton report |
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Full document | 3nla_full_validation.pdf.gz | 654.4 KB | Display | |
Data in XML | 3nla_validation.xml.gz | 64.5 KB | Display | |
Data in CIF | 3nla_validation.cif.gz | 98 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/nl/3nla ftp://data.pdbj.org/pub/pdb/validation_reports/nl/3nla | HTTPS FTP |
-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 7885.328 Da / Num. of mol.: 1 / Fragment: N-TERMINAL DOMAIN WITH A LINKER PORTION Source method: isolated from a genetically manipulated source Source: (gene. exp.) Lycodichthys dearborni (Antarctic eel pout) Tissue: BLOOD PLASMA / Organ: BLOOD / Variant: RD3 / Plasmid: PKK223-3UC / Production host: Escherichia coli (E. coli) / Strain (production host): JM105 / References: UniProt: P35753 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||
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NMR experiment |
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NMR details | Text: THE STRUCTURE WAS DETERMINED USING DOUBLE-RESONANCE NMR SPECTROSCOPY ON 15N-LABELED RD3-NL |
-Sample preparation
Sample conditions | pH: 6.7 / Temperature: 277 K |
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Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
NMR spectrometer |
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-Processing
Software |
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NMR software |
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Refinement | Method: SA / Software ordinal: 1 Details: REFINEMENT DETAILS CAN BE FOUND IN THE JRNL CITATION ABOVE. | ||||||||||||
NMR ensemble | Conformer selection criteria: LEAST RESTRAINT VIOLATION / Conformers calculated total number: 100 / Conformers submitted total number: 40 |