分子量: 15800.444 Da / 分子数: 1 / 由来タイプ: 合成 詳細: sequence occurs naturally in S.cerevisiae and was synthesized in vitro using T7 polymerase and synthetic DNA oligonucleotides 由来: (合成) Saccharomyces cerevisiae (パン酵母)
-
実験情報
-
実験
実験
手法: 溶液NMR
NMR実験
Conditions-ID
Experiment-ID
Solution-ID
タイプ
1
1
1
2D 1H-1H TOCSY
1
2
1
2D 1H-1H NOESY
1
3
2
2D 1H-1H NOESY
2
4
4
2D 1H-1H NOESY
2
5
5
2D 1H-15N HSQC
1
6
3
2D 1H-13C HSQC
1
7
1
3D 1H-13C NOESY aliphatic
2
8
5
2D JNN HNN COSY
1
9
3
2D 1H-15N HSQC
-
試料調製
詳細
Solution-ID
内容
溶媒系
1
0.3-1.0 mM RNA (49-MER), 60 mM potassium chloride, 10 uM EDTA, 0-12 mM magnesium chloride, 100% D2O
100% D2O
2
0.3-1.0 mM [3',4',5',5 ,5-D5] RNA (49-MER), 60 mM potassium chloride, 10 uM EDTA, 0-12 mM magnesium chloride, 100% D2O
100% D2O
3
0.3-1 mM 100% 13C; 100% 15N RNA (49-MER), 60 mM potassium chloride, 10 uM EDTA, 0-12 mM magnesium chloride, 100% D2O
100% D2O
4
0.3-1.0 mM RNA (49-MER), 60 mM potassium chloride, 10 uM EDTA, 0-12 mM magnesium chloride, 90% H2O/10% D2O
90% H2O/10% D2O
5
0.3-1.0 mM 100% 13C; 100% 15N RNA (49-MER), 60 mM potassium chloride, 10 uM EDTA, 0-12 mM magnesium chloride, 90% H2O/10% D2O
NOE constraints total: 848 / NOE intraresidue total count: 256 / NOE long range total count: 134 / NOE medium range total count: 38 / NOE sequential total count: 420
代表構造
選択基準: lowest energy
NMRアンサンブル
コンフォーマー選択の基準: structures with the lowest energy 計算したコンフォーマーの数: 200 / 登録したコンフォーマーの数: 20 / Maximum lower distance constraint violation: 0.2 Å / Maximum upper distance constraint violation: 0.2 Å