[English] 日本語
Yorodumi- PDB-2ltk: Solution structure of a monomeric truncated mutant of Trypanosoma... -
+Open data
-Basic information
Entry | Database: PDB / ID: 2ltk | ||||||
---|---|---|---|---|---|---|---|
Title | Solution structure of a monomeric truncated mutant of Trypanosoma brucei 1-C-Grx1 | ||||||
Components | Mono-cysteine glutaredoxin | ||||||
Keywords | OXIDOREDUCTASE / monothiol glutaredoxin | ||||||
Function / homology | Function and homology information | ||||||
Biological species | Trypanosoma brucei (eukaryote) | ||||||
Method | SOLUTION NMR / molecular dynamics | ||||||
Authors | Pavan, C. / Gesiot, L. / Sturlese, M. / Mammi, S. / Manta, B. / Comini, M. / Bellanda, M. | ||||||
Citation | Journal: Antioxid Redox Signal / Year: 2013 Title: Iron-Sulfur Cluster Binding by Mitochondrial Monothiol Glutaredoxin-1 of Trypanosoma brucei: Molecular Basis of Iron-Sulfur Cluster Coordination and Relevance for Parasite Infectivity. Authors: Manta, B. / Pavan, C. / Sturlese, M. / Medeiros, A. / Crispo, M. / Berndt, C. / Krauth-Siegel, R.L. / Bellanda, M. / Comini, M.A. | ||||||
History |
|
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
---|
-Downloads & links
-Download
PDBx/mmCIF format | 2ltk.cif.gz | 675.8 KB | Display | PDBx/mmCIF format |
---|---|---|---|---|
PDB format | pdb2ltk.ent.gz | 589 KB | Display | PDB format |
PDBx/mmJSON format | 2ltk.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/lt/2ltk ftp://data.pdbj.org/pub/pdb/validation_reports/lt/2ltk | HTTPS FTP |
---|
-Related structure data
Similar structure data | |
---|---|
Other databases |
-Links
-Assembly
Deposited unit |
| |||||||||
---|---|---|---|---|---|---|---|---|---|---|
1 |
| |||||||||
NMR ensembles |
|
-Components
#1: Protein | Mass: 12325.451 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Trypanosoma brucei (eukaryote) / Gene: mgrx / Plasmid: pET Trx-1b / Production host: Escherichia coli (E. coli) / Strain (production host): BL21(DE3) / References: UniProt: Q2UZM9 |
---|
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
NMR experiment |
|
-Sample preparation
Details | Contents: 1 mM [U-13C; U-15N] Tb 1-C-Grx1, 50 mM sodium phosphate, 150 mM sodium chloride, 10 mM DTT, 90% H2O/10% D2O Solvent system: 90% H2O/10% D2O | ||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Sample |
| ||||||||||||||||||||
Sample conditions | pH: 7.0 / Pressure: ambient / Temperature: 298 K |
-NMR measurement
NMR spectrometer |
|
---|
-Processing
NMR software |
| ||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method: molecular dynamics / Software ordinal: 1 | ||||||||||||||||||||||||||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||||||||||||||||||||||||||
NMR ensemble | Conformer selection criteria: target function / Conformers calculated total number: 100 / Conformers submitted total number: 20 |