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- PDB-2lsp: solution structures of BRD4 second bromodomain with NF-kB-K310ac ... -

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Basic information

Entry
Database: PDB / ID: 2lsp
Titlesolution structures of BRD4 second bromodomain with NF-kB-K310ac peptide
Components
  • Bromodomain-containing protein 4
  • NF-kB-K310ac peptide
KeywordsPROTEIN BINDING / NF-kB / BRD4 / HIV / bromodomain / kidney disease
Function / homology
Function and homology information


prolactin signaling pathway / DEx/H-box helicases activate type I IFN and inflammatory cytokines production / NF-kappaB p50/p65 complex / toll-like receptor TLR6:TLR2 signaling pathway / IkBA variant leads to EDA-ID / positive regulation of Schwann cell differentiation / cellular response to peptidoglycan / ankyrin repeat binding / Regulated proteolysis of p75NTR / CLEC7A/inflammasome pathway ...prolactin signaling pathway / DEx/H-box helicases activate type I IFN and inflammatory cytokines production / NF-kappaB p50/p65 complex / toll-like receptor TLR6:TLR2 signaling pathway / IkBA variant leads to EDA-ID / positive regulation of Schwann cell differentiation / cellular response to peptidoglycan / ankyrin repeat binding / Regulated proteolysis of p75NTR / CLEC7A/inflammasome pathway / SUMOylation of immune response proteins / RIP-mediated NFkB activation via ZBP1 / postsynapse to nucleus signaling pathway / negative regulation of protein sumoylation / Interleukin-1 processing / nucleotide-binding oligomerization domain containing 2 signaling pathway / defense response to tumor cell / cellular response to interleukin-6 / actinin binding / response to UV-B / non-canonical NF-kappaB signal transduction / negative regulation of non-canonical NF-kappaB signal transduction / positive regulation of miRNA metabolic process / Regulation of NFE2L2 gene expression / positive regulation of leukocyte adhesion to vascular endothelial cell / interleukin-1-mediated signaling pathway / signal transduction involved in regulation of gene expression / NF-kappaB complex / vascular endothelial growth factor signaling pathway / toll-like receptor 4 signaling pathway / positive regulation of amyloid-beta formation / cellular response to hepatocyte growth factor stimulus / response to cobalamin / phosphate ion binding / positive regulation of T cell receptor signaling pathway / cellular response to lipoteichoic acid / response to muramyl dipeptide / RNA polymerase II C-terminal domain binding / TRAF6 mediated NF-kB activation / P-TEFb complex binding / negative regulation of DNA damage checkpoint / positive regulation of vascular endothelial growth factor production / Transcriptional Regulation by VENTX / cellular response to angiotensin / The NLRP3 inflammasome / histone H4 reader activity / general transcription initiation factor binding / cellular response to interleukin-1 / host-mediated suppression of viral transcription / hair follicle development / canonical NF-kappaB signal transduction / neuropeptide signaling pathway / NF-kappaB binding / response to amino acid / cellular defense response / positive regulation of T-helper 17 cell lineage commitment / RNA polymerase II core promoter sequence-specific DNA binding / Purinergic signaling in leishmaniasis infection / response to cAMP / NF-kB is activated and signals survival / tumor necrosis factor-mediated signaling pathway / positive regulation of G2/M transition of mitotic cell cycle / response to muscle stretch / response to cytokine / positive regulation of interleukin-12 production / antiviral innate immune response / peptide binding / negative regulation of cytokine production involved in inflammatory response / RNA polymerase II CTD heptapeptide repeat kinase activity / CD209 (DC-SIGN) signaling / : / response to interleukin-1 / negative regulation of insulin receptor signaling pathway / negative regulation of miRNA transcription / response to progesterone / negative regulation of angiogenesis / Dectin-1 mediated noncanonical NF-kB signaling / condensed nuclear chromosome / Turbulent (oscillatory, disturbed) flow shear stress activates signaling by PIEZO1 and integrins in endothelial cells / animal organ morphogenesis / positive regulation of interleukin-1 beta production / Activation of NF-kappaB in B cells / response to ischemia / negative regulation of extrinsic apoptotic signaling pathway / positive regulation of interleukin-8 production / TAK1-dependent IKK and NF-kappa-B activation / transcription coregulator activity / RNA polymerase II transcription regulatory region sequence-specific DNA binding / positive regulation of transcription elongation by RNA polymerase II / chromatin DNA binding / protein catabolic process / liver development / positive regulation of non-canonical NF-kappaB signal transduction / CLEC7A (Dectin-1) signaling / positive regulation of NF-kappaB transcription factor activity / negative regulation of protein catabolic process / response to insulin / FCERI mediated NF-kB activation / Interleukin-1 signaling / PKMTs methylate histone lysines
Similarity search - Function
Transcription factor RelA (p65) / NF-kappa-B/Dorsal / Rel homology domain, conserved site / NFkappaB IPT domain / NF-kappa-B/Rel/dorsal domain signature. / Rel homology domain (RHD), DNA-binding domain / Rel homology dimerisation domain / Rel homology DNA-binding domain / Rel homology dimerisation domain / NF-kappa-B/Rel/dorsal domain profile. ...Transcription factor RelA (p65) / NF-kappa-B/Dorsal / Rel homology domain, conserved site / NFkappaB IPT domain / NF-kappa-B/Rel/dorsal domain signature. / Rel homology domain (RHD), DNA-binding domain / Rel homology dimerisation domain / Rel homology DNA-binding domain / Rel homology dimerisation domain / NF-kappa-B/Rel/dorsal domain profile. / Rel homology domain (RHD), DNA-binding domain superfamily / ig-like, plexins, transcription factors / IPT domain / Bromodomain protein 4, C-terminal / C-terminal domain of bromodomain protein 4 / p53-like transcription factor, DNA-binding / Brdt, bromodomain, repeat I / Brdt, bromodomain, repeat II / NET domain superfamily / NET domain profile. / : / NET domain / Bromodomain extra-terminal - transcription regulation / Bromodomain-like / Histone Acetyltransferase; Chain A / Bromodomain, conserved site / Bromodomain signature. / Bromodomain / bromo domain / Bromodomain / Bromodomain (BrD) profile. / Bromodomain-like superfamily / Immunoglobulin E-set / Immunoglobulin-like fold / Up-down Bundle / Mainly Alpha
Similarity search - Domain/homology
Bromodomain-containing protein 4 / Transcription factor p65
Similarity search - Component
Biological speciesHomo sapiens (human)
Homo Sapiens (human)
MethodSOLUTION NMR / DGSA-distance geometry simulated annealing, torsion angle dynamics
AuthorsZhang, G. / Liu, R. / Zhong, Y. / Plotnikov, A.N. / Zhang, W. / Rusinova, E. / Gerona-Nevarro, G. / Moshkina, N. / Joshua, J. / Chuang, P.Y. ...Zhang, G. / Liu, R. / Zhong, Y. / Plotnikov, A.N. / Zhang, W. / Rusinova, E. / Gerona-Nevarro, G. / Moshkina, N. / Joshua, J. / Chuang, P.Y. / Ohlmeyer, M. / He, J. / Zhou, M.-M.
CitationJournal: J.Biol.Chem. / Year: 2012
Title: Down-regulation of NF-kappa B transcriptional activity in HIV-associated kidney disease by BRD4 inhibition.
Authors: Zhang, G. / Liu, R. / Zhong, Y. / Plotnikov, A.N. / Zhang, W. / Zeng, L. / Rusinova, E. / Gerona-Nevarro, G. / Moshkina, N. / Joshua, J. / Chuang, P.Y. / Ohlmeyer, M. / He, J.C. / Zhou, M.M.
History
DepositionMay 3, 2012Deposition site: BMRB / Processing site: RCSB
Revision 1.0Jul 18, 2012Provider: repository / Type: Initial release
Revision 1.1Aug 28, 2013Group: Database references
Revision 2.0Nov 15, 2023Group: Atomic model / Data collection ...Atomic model / Data collection / Database references / Derived calculations
Category: atom_site / chem_comp_atom ...atom_site / chem_comp_atom / chem_comp_bond / database_2 / pdbx_nmr_spectrometer / struct_conn
Item: _atom_site.auth_atom_id / _atom_site.label_atom_id ..._atom_site.auth_atom_id / _atom_site.label_atom_id / _database_2.pdbx_DOI / _database_2.pdbx_database_accession / _pdbx_nmr_spectrometer.model / _struct_conn.pdbx_leaving_atom_flag
Revision 2.1Nov 6, 2024Group: Database references / Structure summary
Category: database_2 / pdbx_entry_details / pdbx_modification_feature
Item: _database_2.pdbx_DOI

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

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Assembly

Deposited unit
A: NF-kB-K310ac peptide
B: Bromodomain-containing protein 4


Theoretical massNumber of molelcules
Total (without water)16,5062
Polymers16,5062
Non-polymers00
Water00
1


  • Idetical with deposited unit
  • defined by author
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
NMR ensembles
DataCriteria
Number of conformers (submitted / calculated)20 / 200structures with the lowest energy
RepresentativeModel #1lowest energy

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Components

#1: Protein/peptide NF-kB-K310ac peptide


Mass: 1663.956 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) Homo Sapiens (human) / References: UniProt: Q04206
#2: Protein Bromodomain-containing protein 4 / Protein HUNK1


Mass: 14842.058 Da / Num. of mol.: 1 / Fragment: second bromodomain
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: BRD4, HUNK1 / Production host: Escherichia coli (E. coli) / Strain (production host): BL21(DE3) / References: UniProt: O60885
Has protein modificationY

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Experimental details

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Experiment

ExperimentMethod: SOLUTION NMR
NMR experiment
Conditions-IDExperiment-IDSolution-IDType
1123D HN(CA)CB
1223D HN(COCA)CB
1323D 1H-15N NOESY
1413D 1H-13C NOESY aliphatic
1513D 1H-13C NOESY aromatic
1613D 13C-Edited-15N/13C filtered NOESY

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Sample preparation

Details
Solution-IDContentsSolvent system
1100 mM sodium phosphate, 5 mM [U-100% 2H] DTT, 100% D2O100% D2O
2100 mM sodium phosphate, 5 mM [U-100% 2H] DTT, 90% H2O/10% D2O90% H2O/10% D2O
Sample
Conc. (mg/ml)ComponentIsotopic labelingSolution-ID
100 mMsodium phosphate-11
5 mMDTT-2[U-100% 2H]1
100 mMsodium phosphate-32
5 mMDTT-4[U-100% 2H]2
Sample conditionspH: 6.5 / Pressure: ambient / Temperature: 298 K

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NMR measurement

NMR spectrometer
TypeManufacturerModelField strength (MHz)Spectrometer-ID
Bruker AvanceBrukerAVANCE8001
Bruker AvanceBrukerAVANCE9002
Bruker AvanceBrukerAVANCE6003
Bruker DRXBrukerDRX5004

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Processing

NMR software
NameVersionDeveloperClassification
ARIA2.3Linge, O'Donoghue and Nilgesrefinement
NMRPipe7.1Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Baxprocessing
NMRPipe7.1Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Baxchemical shift calculation
TALOS3.70F1Cornilescu, Delaglio and Baxgeometry optimization
NMRView5.04Johnson, One Moon Scientificchemical shift assignment
NMRView5.04Johnson, One Moon Scientificdata analysis
NMRView5.04Johnson, One Moon Scientificpeak picking
CNS1.21Brunger, Adams, Clore, Gros, Nilges and Readstructure solution
CNSBrunger, Adams, Clore, Gros, Nilges and Readrefinement
RefinementMethod: DGSA-distance geometry simulated annealing, torsion angle dynamics
Software ordinal: 1
NMR representativeSelection criteria: lowest energy
NMR ensembleConformer selection criteria: structures with the lowest energy
Conformers calculated total number: 200 / Conformers submitted total number: 20

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