- PDB-2lsj: Solution structure of the mouse Rev1 CTD in complex with the Rev1... -
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基本情報
登録情報
データベース: PDB / ID: 2lsj
タイトル
Solution structure of the mouse Rev1 CTD in complex with the Rev1-interacting Region (RIR)of Pol Kappa
要素
DNA polymerase kappaPOLK
DNA repair protein REV1DNA修復
キーワード
PROTEIN BINDING/PROTEIN BINDING / Rev1 / RIR / Pol Kappa / Translesion Synthesis (DNA修復) / TLS / CTD / PROTEIN BINDING-PROTEIN BINDING complex
機能・相同性
機能・相同性情報
Gap-filling DNA repair synthesis and ligation in GG-NER / Translesion synthesis by REV1 / Translesion synthesis by POLK / Translesion synthesis by POLI / HDR through Homologous Recombination (HRR) / Termination of translesion DNA synthesis / Dual Incision in GG-NER / Dual incision in TC-NER / Gap-filling DNA repair synthesis and ligation in TC-NER / deoxycytidyl transferase activity ...Gap-filling DNA repair synthesis and ligation in GG-NER / Translesion synthesis by REV1 / Translesion synthesis by POLK / Translesion synthesis by POLI / HDR through Homologous Recombination (HRR) / Termination of translesion DNA synthesis / Dual Incision in GG-NER / Dual incision in TC-NER / Gap-filling DNA repair synthesis and ligation in TC-NER / deoxycytidyl transferase activity / nucleotide-excision repair, DNA gap filling / error-free translesion synthesis / site of DNA damage / error-prone translesion synthesis / response to UV / cellular response to UV / 転移酵素; リンを含む基を移すもの; 核酸を移すもの / DNA複製 / damaged DNA binding / DNAポリメラーゼ / molecular adaptor activity / DNA-directed DNA polymerase activity / nuclear body / DNA修復 / DNA damage response / 核質 / 細胞核 / metal ion binding 類似検索 - 分子機能
DNA repair protein Rev1, C-terminal domain / DNA repair protein Rev1, C-terminal / Rev1, C-terminal domain superfamily / : / DNA repair protein REV1 C-terminal domain / HUWE1/Rev1, ubiquitin binding region / Ubiquitin binding region / Rad18-like CCHC zinc finger / DNA repair protein Rev1 / DNA polymerase IV ...DNA repair protein Rev1, C-terminal domain / DNA repair protein Rev1, C-terminal / Rev1, C-terminal domain superfamily / : / DNA repair protein REV1 C-terminal domain / HUWE1/Rev1, ubiquitin binding region / Ubiquitin binding region / Rad18-like CCHC zinc finger / DNA repair protein Rev1 / DNA polymerase IV / DNApol eta/Rev1, HhH motif / Rad18, zinc finger UBZ4-type / Zinc finger UBZ4-type profile. / BRCA1 C Terminus (BRCT) domain / DNA polymerase, Y-family, little finger domain / impB/mucB/samB family C-terminal domain / UmuC domain / DNA polymerase, Y-family, little finger domain superfamily / impB/mucB/samB family / UmuC domain profile. / breast cancer carboxy-terminal domain / BRCT domain profile. / BRCT domain / BRCT domain superfamily / Methane Monooxygenase Hydroxylase; Chain G, domain 1 / Reverse transcriptase/Diguanylate cyclase domain / DNA/RNA polymerase superfamily / Up-down Bundle / Mainly Alpha 類似検索 - ドメイン・相同性
DNA repair protein REV1 / DNA polymerase kappa 類似検索 - 構成要素
0.7-1 mM [U-100% 15N] protein, 0.7-1 mM [U-100% 15N] peptide, 25 mM sodium phosphate, 100 mM potassium chloride, 10 mM DTT, 1 mM EDTA, 90% H2O/10% D2O
90% H2O/10% D2O
2
0.7-1 mM [U-100% 13C; U-100% 15N] protein, 0.7-1 mM [U-100% 13C; U-100% 15N] peptide, 25 mM sodium phosphate, 100 mM potassium chloride, 10 mM DTT, 1 mM EDTA, 90% H2O/10% D2O
90% H2O/10% D2O
3
0.7-1 mM [U-100% 13C; U-100% 15N] protein, 0.7-1 mM [U-100% 13C; U-100% 15N] peptide, 25 mM sodium phosphate, 100 mM potassium chloride, 10 mM DTT, 1 mM EDTA, 100% D2O
100% D2O
試料
濃度 (mg/ml)
単位
構成要素
Isotopic labeling
Conc. range (mg/ml)
Solution-ID
mM
entity_1-1
[U-100% 15N]
0.7-1
1
mM
entity_2-2
[U-100% 15N]
0.7-1
1
25mM
sodium phosphate-3
1
100mM
potassium chloride-4
1
10mM
DTT-5
1
1mM
EDTA-6
1
mM
entity_1-7
[U-100% 13C; U-100% 15N]
0.7-1
2
mM
entity_2-8
[U-100% 13C; U-100% 15N]
0.7-1
2
25mM
sodium phosphate-9
2
100mM
potassium chloride-10
2
10mM
DTT-11
2
1mM
EDTA-12
2
mM
entity_1-13
[U-100% 13C; U-100% 15N]
0.7-1
3
mM
entity_2-14
[U-100% 13C; U-100% 15N]
0.7-1
3
25mM
sodium phosphate-15
3
100mM
potassium chloride-16
3
10mM
DTT-17
3
1mM
EDTA-18
3
試料状態
イオン強度: 0.1 / pH: 7.0 / 圧: ambient / 温度: 310 K
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NMR測定
NMRスペクトロメーター
タイプ
製造業者
モデル
磁場強度 (MHz)
Spectrometer-ID
Varian INOVA
Varian
INOVA
600
1
Varian INOVA
Varian
INOVA
800
2
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解析
NMR software
名称
開発者
分類
TALOS
Cornilescu, DelaglioandBax
データ解析
Sparky
Goddard
データ解析
CYANA
Guntert, MumenthalerandWuthrich
構造決定
CYANA
Guntert, MumenthalerandWuthrich
精密化
精密化
手法: simulated annealing / ソフトェア番号: 1
代表構造
選択基準: lowest energy
NMRアンサンブル
コンフォーマー選択の基準: target function / 計算したコンフォーマーの数: 100 / 登録したコンフォーマーの数: 20