+Open data
-Basic information
Entry | Database: PDB / ID: 2ls0 | ||||||
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Title | Solution Structure of the Target Recognition Domain of Zoocin A | ||||||
Components | Zoocin A endopeptidase | ||||||
Keywords | HYDROLASE / specificity | ||||||
Function / homology | Function and homology information lysostaphin / septum digestion after cytokinesis / : / cell division site / cell outer membrane / metalloendopeptidase activity Similarity search - Function | ||||||
Biological species | Streptococcus equi subsp. zooepidemicus (bacteria) | ||||||
Method | SOLUTION NMR / simulated annealing, molecular dynamics | ||||||
Model details | lowest energy, model 1 | ||||||
Authors | Timkovich, R. / Chen, Y. / Simmonds, R.S. | ||||||
Citation | Journal: Proteins / Year: 2013 Title: Solution structure of the recombinant target recognition domain of zoocin A. Authors: Chen, Y. / Simmonds, R.S. / Young, J.K. / Timkovich, R. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2ls0.cif.gz | 387.3 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2ls0.ent.gz | 320.2 KB | Display | PDB format |
PDBx/mmJSON format | 2ls0.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 2ls0_validation.pdf.gz | 407.6 KB | Display | wwPDB validaton report |
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Full document | 2ls0_full_validation.pdf.gz | 519.5 KB | Display | |
Data in XML | 2ls0_validation.xml.gz | 59.5 KB | Display | |
Data in CIF | 2ls0_validation.cif.gz | 80.2 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ls/2ls0 ftp://data.pdbj.org/pub/pdb/validation_reports/ls/2ls0 | HTTPS FTP |
-Related structure data
Similar structure data | |
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Other databases |
-Links
-Assembly
Deposited unit |
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NMR ensembles |
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-Components
#1: Protein | Mass: 14089.651 Da / Num. of mol.: 1 / Fragment: UNP residues 170-285 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Streptococcus equi subsp. zooepidemicus (bacteria) Gene: zooA / Production host: Escherichia coli (E. coli) / References: UniProt: O54308 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Details |
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Sample |
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Sample conditions | Ionic strength: 0.02 / pH: 7.0 / Pressure: ambient / Temperature: 297 K |
-NMR measurement
NMR spectrometer | Type: Bruker Avance / Manufacturer: Bruker / Model: AVANCE / Field strength: 600 MHz |
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-Processing
NMR software |
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Refinement | Method: simulated annealing, molecular dynamics / Software ordinal: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 100 / Conformers submitted total number: 10 |