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Yorodumi- PDB-2lrw: Solution structure of a ubiquitin-like protein from Trypanosoma brucei -
+Open data
-Basic information
Entry | Database: PDB / ID: 2lrw | ||||||
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Title | Solution structure of a ubiquitin-like protein from Trypanosoma brucei | ||||||
Components | Ubiquitin, putative | ||||||
Keywords | CELL CYCLE / POSTTRANSLATIONAL PROTEIN MODIFIER | ||||||
Function / homology | Function and homology information regulation of proteolysis / protein neddylation / cilium / modification-dependent protein catabolic process / protein tag activity / ubiquitin protein ligase binding / nucleus / cytosol Similarity search - Function | ||||||
Biological species | Trypanosoma brucei brucei (eukaryote) | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
Model details | lowest energy, model1 | ||||||
Authors | Wang, R. / Wang, T. / Liao, S. / Zhang, J. / Tu, X. | ||||||
Citation | Journal: To be Published Title: Solution structure of a ubiqutin-like protein from Trypanosoma bucei Authors: Wang, R. / Liao, S. / Wang, T. / Zhang, J. / Tu, X. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2lrw.cif.gz | 553.2 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2lrw.ent.gz | 468.5 KB | Display | PDB format |
PDBx/mmJSON format | 2lrw.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 2lrw_validation.pdf.gz | 534.7 KB | Display | wwPDB validaton report |
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Full document | 2lrw_full_validation.pdf.gz | 712.2 KB | Display | |
Data in XML | 2lrw_validation.xml.gz | 34.1 KB | Display | |
Data in CIF | 2lrw_validation.cif.gz | 48.7 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/lr/2lrw ftp://data.pdbj.org/pub/pdb/validation_reports/lr/2lrw | HTTPS FTP |
-Related structure data
Similar structure data | |
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Other databases |
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 9509.005 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: 927/4 GUTat10.1 / Gene: Tb927.4.2540 / Production host: Escherichia coli (E. coli) / References: UniProt: Q584D3 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Details | Contents: 0.5 mM [U-100% 13C; U-100% 15N] tbubl-1, 90% H2O/10% D2O Solvent system: 90% H2O/10% D2O |
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Sample | Conc.: 0.5 mM / Component: tbubl-1 / Isotopic labeling: [U-100% 13C; U-100% 15N] |
Sample conditions | Ionic strength: 0.15 / pH: 6.8 / Pressure: ambient / Temperature: 293 K |
-NMR measurement
NMR spectrometer | Type: Bruker DMX / Manufacturer: Bruker / Model: DMX / Field strength: 500 MHz |
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-Processing
NMR software |
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Refinement | Method: simulated annealing / Software ordinal: 1 | |||||||||
NMR representative | Selection criteria: lowest energy | |||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 500 / Conformers submitted total number: 20 / Representative conformer: 1 |