3D simutaneous 13C-aromatic,13C-aliphatic,15N edited 1H-1H NOESY
1
7
2
2D 1H-13C HSQC
1
8
1
3DHBHA(CO)NH
1
9
1
3D (H)CCH-COSY
1
10
1
3D HNCA
1
11
1
3DHN(CO)CA
1
12
1
3DHN(CA)CO
-
試料調製
詳細
Solution-ID
内容
溶媒系
1
0.89 mM [U-100% 13C; U-100% 15N] SnR212.015, 0.02 % NaN3, 10 mM DTT, 5 mM CaCL2, 100 mM NaCL, 1 x Proteinase Inhibitors, 20 mM MES pH 6.5, 10 % D2O, 50 uM DSS, 90% H2O/10% D2O
90% H2O/10% D2O
2
0.67mM [U-5% 13C; U-100% 15N] SnR212.015, 0.02 % NaN3, 10 mM DTT, 5 mM CaCL2, 100 mM NaCL, 1 x Proteinase Inhibitors, 20 mM MES pH 6.5, 10 % D2O, 50 uM DSS, 90% H2O/10% D2O